A5008357467- Trypanosoma species research and implications
- Synthesis and Biological Evaluation
- Research on Leishmaniasis Studies
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Biochemical and Molecular Research
- Bioactive Compounds and Antitumor Agents
- Cannabis and Cannabinoid Research
- Metal complexes synthesis and properties
- Contact Dermatitis and Allergies
- Asthma and respiratory diseases
- Occupational exposure and asthma
- Photosynthetic Processes and Mechanisms
- Vanadium and Halogenation Chemistry
- Respiratory Support and Mechanisms
- Synthesis and biological activity
- Toxin Mechanisms and Immunotoxins
- X-ray Diffraction in Crystallography
- Insect Pest Control Strategies
- Pleural and Pulmonary Diseases
- Fermentation and Sensory Analysis
- Metal-Catalyzed Oxygenation Mechanisms
- Crystallization and Solubility Studies
- Cholinesterase and Neurodegenerative Diseases
- Diet, Metabolism, and Disease
Hospital Padre Hurtado
2024
Clínica Alemana
2024
Universidad de la República
2011-2020
Universidad de Santiago de Chile
2014-2016
Rede de Química e Tecnologia
2015
Instituto de Biologia Experimental e Tecnológica
2015
Universidad La República
2011-2015
Centro Uruguayo de Imagenología Molecular
2014
Centro de Investigaciones Biológicas Margarita Salas
1990-2013
Consejo Superior de Investigaciones Científicas
1992-2008
Summary Background Allergic reactions to pea ( Pisum sativum ) ingestion are frequently associated with lentil allergy in the Spanish population. Vicilin have been described as a major allergen. Objective To identify main IgE binding components from seeds and study their potential cross‐reactivity vicilin. Methods A serum pool or individual sera 18 patients were used detect proteins by immunodetection immunoblot inhibition assays. Protein preparations enriched vicilin obtained gel filtration...
The biochemical and immunochemical characterization of a superoxide dismutase (SOD, EC 1.15.1.1) from peroxisomal origin has been carried out. enzyme is Cu,Zn-containing SOD (CuZn-SOD) located in the matrix peroxisomes watermelon (Citrullus vulgaris Schrad.) cotyledons (L.M. Sandalio L.A. del Rio [1988] Plant Physiol 88: 1215–1218). amino acid composition was determined. Analysis by reversed-phase high-performance liquid chromatography CuZn-SOD incubated with 6 M guanidine-HCI indicated that...
Summary Background Green kiwifruit allergy is on the rise. However, no surveys testing purified major kiwi allergens have been carried out in a large population, including both kiwi‐sensitized [skin prick test (SPT)‐positive] and truly kiwi‐allergic patients. Objective To isolate allergens, to explore their relevance by vitro vivo methods ‐allergic population. Methods A group ( n =92) of patients with different clinical symptoms were selected, double‐blind, placebo‐controlled, food...
Since intestinal absorption of food protein can trigger an allergic reaction, the effect plant allergen on epithelial cell permeability and its ability to cross monolayer was evaluated.To study interaction Pru p 3 with epithelium, natural entrance, analyzing transport kinetics cellular responses that trigger.This achieved using 3, peach LTP, as a model. Enterocytic monolayers were established by culturing Caco 2 cells, model enterocytes, permeable supports separate apical basal compartments....
Searching for prospective metal-based drugs the treatment of Chagas disease, a new series ten mixed-ligand oxidovanadium(IV) complexes, [VIVO(L-2H)(NN)], where L is tridentate salicylaldehyde semicarbazone derivative (L1–L5) and NN either 5-amine-1,10-phenanthroline (aminophen) or 5,6-epoxy-5,6-dihydro-1,10-phenanthroline (epoxyphen), were synthesized. The compounds characterized in solid state solution. EPR spectroscopy suggests that ligands act as bidentate through both nitrogen donor...
Summary Background Baker's asthma is an important occupational allergic disease. Wheat lipid transfer protein (LTP) Tri a 14 major allergen associated with wheat allergy. No panel of recombinant allergens for component‐resolved diagnosis baker's currently available. Objective To evaluate the potential role as novel tool asthma, and to test heat proteolytic resistance LTP allergen. Methods A cDNA encoding was isolated sequenced, produced in Pichia pastoris purified by chromatographic methods....
Chagas disease, caused by Trypanosoma cruzi parasite, was described thousands of years ago. Currently, it affects millions people, mostly in Latin America, and there are not suitable drugs for treating it. As an attempt to find appropriate deal with this problem, we report here on the design, synthesis, characterization 82 new compounds. Trypanosomicidal behavior vitro showed more than 20 outstanding derivatives anti-Trypanosoma activity. Furthermore, studied nonspecific toxicity against...
ABSTRACT Although the parasitic infection Chagas' disease was described over 100 years ago, even now there are not suitable drugs. The available drugs nifurtimox and benznidazole have limited efficacies tolerances, with proven mutagenic effects. Attempting to find appropriate deal this problem, here we report on development pharmacological characterization of new amide-containing thiazoles. In present study, evaluated in vitro vivo effects candidates against Trypanosoma cruzi , etiological...
Objectives To assess the efficacy of desmopressin nasal spray compared with diclofenac given intramuscularly in patients acute renal colic caused by urolithiasis. Patients and methods The study included 61 randomized into three different groups; group A received (40 µg, spray), B (75 mg) C, both diclofenac. Pain was assessed using a visual analogue scale (a 10‐cm horizontal ranging from ‘no pain’ to ‘unbearable pain’) at baseline, 10, 20 30 min after administering treatments. Results On...
Abstract Triosephosphate isomerase (TIM) is an essential Trypanosoma cruzi enzyme and one of the few validated drug targets for Chagas disease. The known inhibitors this behave poorly or have low activity in parasite. In work, we used symmetrical diarylideneketones derived from structures with trypanosomicidal activity. We obtained enzymatic inhibitor IC 50 value 86 n m without inhibition effects on mammalian enzyme. These molecules also affected cruzipain, another proteolytic This dual...
As part of our efforts to develop organometallic ruthenium compounds bearing activity on both trypanosomatid parasites and tumor cells, a new Ru(II)–cyclopentadienyl clotrimazole complex, [RuCp(PPh3)2(CTZ)](CF3SO3), where Cp = cyclopentadienyl, CTZ clotrimazole, was synthesized characterized. The compound evaluated in vitro T. cruzi (Y strain), the infective form brucei strain 427 (cell line 449), three human cell lines with different sensitivity cisplatin (A2780, ovary; MCF7, breast; HeLa,...
A set of new aryloxy-quinones were synthesized and evaluated<italic>in vitro</italic>against the epimastigote form of<italic>Trypanosoma cruzi</italic>and their unspecific cytotoxicity was tested on murine macrophages J-774 cells.
The currently available treatments for Chagas disease show limited therapeutic potential and are associated with serious side effects. Attempting to find alternative drugs isolated from Nature as agents against Trypanosoma cruzi has been our goal. Recently, we have demonstrated the in vitro anti-T. activities of two secondary metabolites hydro-ethanolic extract aerial parts Aristeguietia glutinosa (Lam.), (family Asteraceae). These active principles displayed poor hemolytic activity, low...
A series of fifty arylideneketones and thiazolidenehydrazines was evaluated against Leishmania infantum braziliensis. Furthermore, new simplified thiazolidenehydrazine derivatives were Trypanosoma cruzi. The cytotoxicity the active compounds on non-infected fibroblasts or macrophages established in vitro to evaluate selectivity their anti-parasitic effects. Seven ten had good activity three parasites. IC50 values for T. cruzi spp. ranged from 90 nM-25 µM. Eight multi-trypanocidal (the...
Dekkera bruxellensis is the major contaminant yeast in wine industry worldwide. Here, we present draft genome sequence of D. LAMAP2480 isolated from a Chilean wine. Genomic evidence reveals shared and exclusive genes potentially involved colonization survival during alcoholic fermentation.