A5103197280- Neonatal Health and Biochemistry
- Metabolism and Genetic Disorders
- Folate and B Vitamins Research
- Biomedical Research and Pathophysiology
- Iron Metabolism and Disorders
- Health Policy Implementation Science
- Hemoglobinopathies and Related Disorders
- Delphi Technique in Research
- Enzyme Structure and Function
- Methemoglobinemia and Tumor Lysis Syndrome
- Health disparities and outcomes
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Optimism, Hope, and Well-being
- Biochemical and Molecular Research
- Psychological Well-being and Life Satisfaction
- Palliative Care and End-of-Life Issues
- Metabolomics and Mass Spectrometry Studies
- Chemical Analysis and Environmental Impact
- Health, psychology, and well-being
- Chromosomal and Genetic Variations
- Nursing Diagnosis and Documentation
- Pharmaceutical industry and healthcare
- Ion Transport and Channel Regulation
- Pharmaceutical Economics and Policy
- Adolescent and Pediatric Healthcare
RELX Group (United States)
2023
Mayo Clinic
2023
University of Cincinnati Medical Center
2022
Cincinnati Children's Hospital Medical Center
2022
University of Hong Kong
1993-2008
Queen Mary Hospital
2004
Chinese University of Hong Kong
1996-2002
Wellcome Sanger Institute
1996
Human glucose-6-phosphate dehydrogenase (G6PD) is NADP+-dependent and catalyses the first rate-limiting step of pentose phosphate shunt. Binary complexes human deletion mutant, ΔG6PD, with NADP+ have been crystallized their structures solved to 2.9 2.5 Å, respectively. The are compared previously determined structure Canton variant G6PD (G6PDCanton) in which bound at structural site. Substrate binding ΔG6PD shown be very similar that described Leuconostoc mesenteroides G6PD. coenzyme site...
The addition of xylose to energy-depleted cells Escherichia coli elicited an alkaline pH change which failed appear in the presence uncoupling agents. Accumulation [14C]xylose by energy-replete was also inhibited agents, but not fluoride or arsenate. Subcellular vesicles E. accumulated provided that ascorbate plus phenazine methosulfate were present for respiration, and this accumulation agents valinomycin. Therefore, transport into appears be energized a proton-motive force, rather than...
Methods of integrating qualitative data across diverse studies and within multi-site research consortia are less developed than those for quantitative data. The development ofsuchmethods is essential to support the exchange needed cross-study inquiry given increasing emphasis on sharing open science. We describe methods integration National Cancer Institute's Improving Management symPtoms During And following Treatment (IMPACT) Consortium funded by MoonshotSM. Data collection analysis were...
Human glucose 6-phosphate dehydrogenase, purified after overexpression in E. coli, was shown to contain one molecule/subunit of acid-extractable "structural" NADP+ and no NADPH. This tightly bound reduced by G6P, presumably following migration the catalytic site. Gel-filtration yielded apoenzyme, devoid but, surprisingly, still fully active. Mr main component "stripped" enzyme gel filtration approximately 100,000, suggesting a dimeric apoenzyme (subunit = 59,000). Holoenzyme also contained...
Cancer and its treatment produce deleterious symptoms across the phases of care. Poorly controlled negatively affect quality life result in increased health-care needs hospitalization. The Improving Management symPtoms during And following Treatment (IMPACT) Consortium was created to develop 3 large-scale, systematic symptom management systems, deployed through electronic health record platforms, test them pragmatic, randomized, hybrid effectiveness implementation trials. Here, we describe...
Recombinant human glucose 6-phosphate dehydrogenase (G6PD) has been crystallized and its structure solved by molecular replacement. Crystals of the natural mutant R459L grow under similar conditions in space groups P212121 C2221 with eight or four 515-residue molecules asymmetric unit, respectively. A non-crystallographic 222 tetramer was found crystal form using a 4 resolution data set dimer large beta + alpha domains Leuconostoc mesenteroides enzyme as search model. This only successful...
A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning analysis each individual product before library screening. series human X hybrids were mapped by this method, using a reference comparisons between overlapping to identify interesting clones further analysis.
G6PDdb (http://www.rubic.rdg.ac.uk/g6pd/ or http://www.bioinf.org.uk/g6pd/) is a newly created web-accessible locus-specific mutation database for the human Glucose-6-phosphate dehydrogenase (G6PD) gene. The relational integrates up-to-date mutational and structural data from various databanks (GenBank, Protein Data Bank, etc.) with biochemically characterized variants their associated phenotypes obtained published literature Favism website. An automated analysis of mutations likely to have...
Clones overexpressing clinical glucose 6-phosphate dehydrogenase (G6PD) mutants Union (c.1360C>T/p.Arg454Cys) and Andalus (c.1361G>A/p.Arg454His), have been constructed. These abolish a salt bridge between Arg454 Asp 286. One mutant is reportedly Class II variant the other I. Kinetic studies of purified proteins reveal that, for both mutants, kcat about 10-fold decreased, thus giving 90% decrease in WHO assay, also presumably under physiological conditions. In contrast with unfavourable...
Background. Age-related skewing of X-chromosome inactivation leading to glucose-6-phosphate dehydrogenase (G6PD) deficiency in elderly women a population with prevalent G6PD gene mutations was investigated.
Cloning and over‐expression of human glucose 6‐phosphate dehydrogenase (Glc6 P dehydrogenase) has for the first time allowed a detailed kinetic study preparation that is genetically homogeneous in which all protein molecules are identical age. The steady‐state kinetics recombinant enzyme, studied by fluorimetric initial‐rate measurements, gave converging linear Lineweaver–Burk plots as expected ternary‐complex mechanism. Patterns product dead‐end inhibition indicated enzyme can bind NADP +...
Journal Article A simplified and improved method for the efficient double-stranded sequencing of mini-prep plasmid DNA Get access W.M. Wong, Wong Department Biochemistry, University Hong KongHong Kong Search other works by this author on: Oxford Academic PubMed Google Scholar D.M.Y. Au, Au V.M.S. Lam, Lam J.W.O. Tam, Tam L.Y.L. Cheng Nucleic Acids Research, Volume 18, Issue 25 September 1990, Page 5573, https://doi.org/10.1093/nar/18.18.5573 Published: 1990 history Received: 06 August
We have studied the spectrum of mutations producting beta-thalassaemia intermedia in South China. The methods mutation detection include oligonucleotide analysis, polymerase chain reaction amplification beta-globin gene and direct genomic sequencing. been identified 22 genes from patients 11 unrelated families. Seven different A to G substitution TATA box accounts for 42% these mutant genes. Most a beta(+) thalassaemia one copy mutation. In two with beta(0) mild phenotype may be explained by...
Objectives Well-being is a holistic, positively framed conception of health, integrating physical, emotional, social, financial, community and spiritual aspects life. High well-being an intrinsically worthy goal for individuals, communities nations. Multiple measures exist, yet we lack information to identify benchmarks, geographical disparities targets intervention improve population life evaluation in the USA. Design Using data from Gallup National Health Well-Being Index, conducted...
Communities are seeking to learn if and how they can improve the well-being of their residents. We therefore examined impact a community-led, collective-impact initiative, deployed through Blue Zones Project by Sharecare, aimed at improving health in one set US communities.We used data from cross-sectional surveys Well-Being Index (2010-2017) assess Life Evaluation (LEI) Hermosa Beach, Manhattan Beach Redondo California (Beach Cities) changed over time this change compares with for similar...
The human X chromosome is associated with a large number of disease phenotypes, principally because its unique mode inheritance that tends to reveal all recessive disorders in males. With the longer term goal identifying and characterizing most these genes, we have adopted chromosome-wide strategy establish YAC contig map. We performed > 3250 inter Alu-PCR product hybridizations identify overlaps between clones. Positional information many clones has been derived from our Reference...
Journal Article Genomic sequence of a Sprague - Dawley rat β-globin gene Get access W.M. Wong, Wong Department Biochemistry, University Hong KongHong Kong Search for other works by this author on: Oxford Academic PubMed Google Scholar V.M.S. Lam, Lam L.Y.L. Cheng, Cheng J.W.O. Tam * To whom reprint requests should be sent Nucleic Acids Research, Volume 16, Issue 5, 11 March 1988, Page 2342, https://doi.org/10.1093/nar/16.5.2342 Published: 1988 history Received: 22 January
Objectives. To describe national- and county-level trends variation in a novel measure of hope. Methods. Using data from the Gallup National Health Well-Being Index (n = 2 766 728), we summarized difference between anticipated life satisfaction (ALS) current (CLS), measured by Cantril Self-Anchoring Scale, for each year 2008 to 2020 county over two 5-year periods United States. Results. Across all years, there was significant positive trend ALS CLS nation (P .024), which remained but not...