A5002745087- HIV Research and Treatment
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Heme Oxygenase-1 and Carbon Monoxide
- Long-Term Effects of COVID-19
- RNA and protein synthesis mechanisms
- Thermal Regulation in Medicine
- RNA modifications and cancer
- RNA Research and Splicing
- HIV-related health complications and treatments
- Complement system in diseases
- Neonatal Health and Biochemistry
- Viral Infections and Immunology Research
- Epigenetics and DNA Methylation
- Hemoglobin structure and function
- HIV/AIDS drug development and treatment
- Heavy Metal Exposure and Toxicity
- Neuroscience and Neuropharmacology Research
- Retinoids in leukemia and cellular processes
- Acute Kidney Injury Research
- Syphilis Diagnosis and Treatment
- Virus-based gene therapy research
- Plant Virus Research Studies
- Trace Elements in Health
- Neurogenesis and neuroplasticity mechanisms
Tulane University
2020-2024
Nepean Hospital
2021
Temple University
2006-2020
Université Savoie Mont Blanc
2020
Cornell University
2020
Université de Rennes
2020
Centre National de la Recherche Scientifique
2020
Keio University
2020
Tsinghua University
2020
University of Pennsylvania
2020
Abstract Understanding SARS-CoV-2 associated immune pathology is crucial to develop pan-effective vaccines and treatments. Here we investigate the events from acute state up four weeks post infection, in non-human primates (NHP) with heterogeneous pulmonary pathology. We show a robust migration of CD16 expressing monocytes lungs occurring during phase, describe two subsets interstitial macrophages (HLA-DR + CD206 − ): transitional CD11c cell population directly IL-6 levels plasma,...
Abstract Renal macrophages (RMs) participate in tissue homeostasis, inflammation and repair. RMs consist of embryo-derived (EMRMs) bone marrow-derived (BMRMs), but the fate, dynamics, replenishment, functions metabolic states these two RM populations remain unclear. Here we investigate characterize at different ages by conditionally labeling ablating several transgenic lines. We find that expand mature parallel with renal growth after birth, are mainly derived from fetal liver monocytes...
Host cell RNA polymerase II-mediated transcription is inhibited by poliovirus infection. Previous studies from our laboratory showed that activated a cyclic AMP-responsive element (CRE)-containing promoter was severely in extracts prepared poliovirus-infected HeLa cells compared to those mock-infected cells. Here we demonstrate the CRE-binding protein, CREB, specifically cleaved poliovirus-encoded protease 3Cpro both vitro and virus-infected The proteolytic cleavage of CREB leads significant...
Human immunodeficiency virus type 1 encoded viral protein Vpr is essential for infection of macrophages by HIV-1. Furthermore, these are resistant to cell death and reservoir. However, the impact on macrophage proteome yet be comprehended. The goal present study was use a stable-isotope labeling amino acids in culture (SILAC) coupled with mass spectrometry-based proteomics approach characterize response macrophages. Cultured human monocytic cells, U937, were differentiated into transduced...
Preclinical mouse models that recapitulate some characteristics of coronavirus disease (COVID-19) will facilitate focused study pathogenesis and virus-host responses. Human agniotensin-converting enzyme 2 (hACE2) serves as an entry receptor for severe acute respiratory syndrome (SARS-CoV-2) to infect people via binding envelope spike proteins. Herein we report development characterization a rapidly deployable COVID-19 model. C57BL/6J (B6) mice expressing hACE2 in the lung were transduced by...
In glomerulonephritis, there is intraglomerular activation of inducible nitric oxide synthase (iNOS) leading to high output production (NO). This can result in supraphysiologic amounts NO and cause oxidative injury. It unknown whether mechanisms cellular defense against NO-mediated injury exist. Induction the heme catabolizing enzyme oxygenase-1 (HO-1), which generates biliverdin, carbon monoxide (CO), iron (Fe), may provide such a mechanism, as CO Fe are two negative modulators iNOS...
HIV-1 infected macrophages play a significant role in the neuropathogenesis of AIDS. viral protein R (Vpr) not only facilitates infection but also contribute to long-lived persistence macrophages. Our previous studies using SILAC-based proteomic analysis showed that expression critical metabolic enzymes glycolytic pathway and tricarboxylic acid (TCA) cycle were altered response Vpr We hypothesized Vpr-induced modulation glycolysis TCA regulates glutamate metabolism release assessed amount...
Abstract Calcium (Ca 2+ ) dynamics and oxidative signaling control mitochondrial bioenergetics in the central nervous system, where astrocytes are a major energy source for neurons. Cocaine use exacerbates HIV-associated neurocognitive disorders, but little is known about disruptions astrocyte metabolism this context. Our data show that HIV protein Tat cocaine induce metabolic switch from glucose to fatty acid oxidation astrocytes, thereby limiting lactate transport Mechanistic analyses...
Viruses have developed various strategies to protect infected cells from apoptosis. HIV-1 macrophages are long-lived and considered reservoirs for HIV-1. One significant deciding factor between cell survival death is glucose metabolism. We hypothesized that protects apoptosis in part by modulating the host glycolytic pathway specifically regulating hexokinase-1 (HK-1) an enzyme converts glucose-6-phosphate. Therefore, we analyzed regulation of HK-1 PBMCs, a chronically monocyte-like line,...
Hyperglycemia, and exacerbation of pre-existing deficits in glucose metabolism, are manifestations the post-acute sequelae SARS-CoV-2. Our understanding metabolic decline after acute COVID-19 remains unclear due to lack animal models. Here, we report a non-human primate model SARS-CoV-2 using infected African green monkeys. Using this model, identify dysregulated blood chemokine signature during that correlates with elevated persistent hyperglycemia four months post-infection. Hyperglycemia...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGene Cloning, Expression, and Characterization of the Sac7 Proteins from Hyperthermophile Sulfolobus acidocaldariusJames G. McAfee, Stephen P. Edmondson, Prasun K. Datta, John W. Shriver, Ramesh GuptaCite this: Biochemistry 1995, 34, 31, 10063–10077Publication Date (Print):August 8, 1995Publication History Published online1 May 2002Published inissue 8 August...
Heme oxygenase-1 (HO-1) has potent anti-inflammatory activity and recognized vascular protective effects. We have recently described the expression effects of an interleukin (IL-19), in smooth muscle cells (VSMC) injured arteries. The objective this study was to link IL-19 with HO-1 resident cells. induced mRNA protein cultured human VSMC, as assayed by quantitative RT-PCR, immunoblot, ELISA. does not induce or endothelial activates STAT3 IL-19-induced is significantly reduced transfection...
Cardiovascular disease is increased in individuals with type 1 or 2 diabetes mellitus (DM). Left ventricular hypertrophy (LVH), which an ominous prognostic sign and independent risk factor for cardiac events, often present DM patients. The aim of our cross-sectional study was to evaluate the prevalence LVH, factors its development, normotensive diabetic patients without antihypertensive medication. objectives were find out high left mass (LVM) compare it nondiabetics uncover development LVM...
HIV-1 Tat protein is released from HIV-1-infected cells and can enter non-permissive including neurons. disrupts neuronal homeostasis may contribute to the neuropathogenesis in people living with HIV (PLWH). The use of cocaine by PLWH exacerbates dysfunction. Here, we examined mechanisms which facilitate alterations homeostatic processes. Bioinformatic interrogation results RNA deep sequencing rat hippocampal neurons exposed alone indicated dysregulation several genes involved lipid...
Elevation in soluble urokinase receptor (suPAR) and proteinuria are common signs patients with moderate to severe coronavirus disease 2019 (COVID-19). Here we characterize a new type of originating as part viral response. Inoculation acute respiratory syndrome 2 (SARS-CoV-2) causes increased suPAR levels glomerulopathy African green monkeys. Using an engineered mouse model high expression, inhaled variants SARS-CoV-2 spike S1 protein elicite that could be blocked by either antibody or...
The poliovirus-encoded, membrane-associated VPg-precursor polypeptide 3AB has been implicated in the initiation of viral RNA synthesis. We have expressed and 3A polypeptides eukaryotic cells examined their localization using indirect immunofluorescence a direct vitro membrane-binding assay. Results presented here demonstrate that both are capable localizing endoplasmic reticulum Golgi apparatus transfected HeLa absence any other poliovirus protein. also shown carboxy-terminal 18 amino acids...
A person’s state of health is a key resource for living fulfilling and rewarding life. The term ‘health’ encompasses physical, mental, social capabilities. Regardless age, depends on his or her ability to maintain homeostasis, adapt adverse situations, relationships. However, many Americans define their own […]