A5109050224- HIV Research and Treatment
- RNA Research and Splicing
- Polyomavirus and related diseases
- RNA Interference and Gene Delivery
- interferon and immune responses
- HIV/AIDS drug development and treatment
- Virus-based gene therapy research
- Full-Duplex Wireless Communications
- NF-κB Signaling Pathways
- RNA regulation and disease
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Prenatal Substance Exposure Effects
- RNA modifications and cancer
- Cancer-related gene regulation
- Plant Virus Research Studies
- Cytomegalovirus and herpesvirus research
- Genomics and Chromatin Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- TGF-β signaling in diseases
- Antenna Design and Analysis
- Herpesvirus Infections and Treatments
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- HIV-related health complications and treatments
Temple University
2016-2025
Temple College
2004-2023
West Virginia University Hospitals
2017
Islamic Azad University, Marvdasht
2016
University of the Sciences
2016
Indiana University School of Medicine
2015
Philadelphia University
2010
University of Nebraska Medical Center
2007
Neurobehavioral Research (United States)
2007
University of California, San Diego
2007
Abstract Elimination of HIV-1 requires clearance and removal integrated proviral DNA from infected cells tissues. Here, sequential long-acting slow-effective release antiviral therapy (LASER ART) CRISPR-Cas9 demonstrate viral in latent infectious reservoirs humanized mice. subgenomic fragments, spanning the long terminal repeats Gag gene, are excised vivo, resulting elimination DNA; virus is not detected blood, lymphoid tissue, bone marrow brain by nested digital-droplet PCR as well RNAscope...
Treatment of HIV-1 ADA -infected CD34+ NSG-humanized mice with long-acting ester prodrugs cabotegravir, lamivudine, and abacavir in combination native rilpivirine was followed by dual CRISPR-Cas9 C-C chemokine receptor type five (CCR5) proviral DNA gene editing. This led to sequential viral suppression, restoration absolute human CD4 + T cell numbers, then elimination replication-competent virus 58% infected mice. Dual CRISPR therapies enabled the excision integrated cells contained within...
Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by JC virus (JCV), human papovavirus. PML relatively rare seen predominantly in immunocompromised individuals and frequent complication observed AIDS patients. The significantly higher incidence patients than other immunosuppressive disorders has suggested that presence immunodeficiency type 1 (HIV-1) brain may directly or indirectly contribute to pathogenesis this disease. In...
Abstract Human immunodeficiency virus-associated neurological disorders (HANDs) affect the majority of AIDS patients and are a significant problem among HIV-1-infected individuals who live longer because combined anti-retroviral therapies. HIV-1 utilizes number viral proteins subsequent cytokine inductions to unleash its toxicity on neurons. Among proteins, Nef is small protein expressed abundantly in astrocytes brains has been suggested have role pathogenesis HAND. In order explore effect...
The single-stranded DNA- and RNA-binding protein, Purα, has been implicated in many biological processes, including control of transcription multiple genes, initiation DNA replication, RNA transport translation. Deletions the PURA gene are frequent acute myeloid leukemia. Mice with targeted disruption both alleles appear normal at birth, but 2 weeks age, they develop neurological problems manifest by severe tremor spontaneous seizures die 4 weeks. There severely lower numbers neurons regions...
The detection of biomarkers oxidative stress in brain tissue and cerebrospinal fluid patients with human immunodeficiency virus, type 1 (HIV)-associated dementia indicates the involvement pathways neuropathogenesis AIDS. Although biological importance on events involved AIDS HIV-1 proteins responsible for remain to be elucidated, our results point activation hypoxia-inducible factor (HIF-1) upon infection its elevation cells dementia. HIF-1 is a transcription that responsive oxygen. Under...
Human immunodeficiency virus type 1 encoded viral protein Vpr is essential for infection of macrophages by HIV-1. Furthermore, these are resistant to cell death and reservoir. However, the impact on macrophage proteome yet be comprehended. The goal present study was use a stable-isotope labeling amino acids in culture (SILAC) coupled with mass spectrometry-based proteomics approach characterize response macrophages. Cultured human monocytic cells, U937, were differentiated into transduced...
The virion-associated protein of human immunodeficiency virus, type 1 (HIV-1), Vpr, is a small with 96 amino acid residues that has the ability to modulate transcription HIV-1 long terminal repeat (LTR) promoter activity and affects several cellular functions. In this study we have employed molecular approaches further investigate mechanism by which Vpr exerts its regulatory effect upon LTR. We show structural functional interaction Tat, potent viral protein, synergistically enhances...
The Tat protein of human immunodeficiency virus type 1 has been increasingly implicated in directly contributing to the disease AIDS by altering expression strategic cellular genes. In this study we demonstrate that presence regulatory is associated with a significant induction certain components extracellular matrix glial-derived cells. Northern blot analysis reveals cells expressing there marked elevation steady-state RNA levels for fibronectin and types I III collagen. Metabolic labeling...
Viruses have developed various strategies to protect infected cells from apoptosis. HIV-1 macrophages are long-lived and considered reservoirs for HIV-1. One significant deciding factor between cell survival death is glucose metabolism. We hypothesized that protects apoptosis in part by modulating the host glycolytic pathway specifically regulating hexokinase-1 (HK-1) an enzyme converts glucose-6-phosphate. Therefore, we analyzed regulation of HK-1 PBMCs, a chronically monocyte-like line,...
The MB1 regulatory sequence of the myelin basic protein (MBP) gene spanning between nucleotides -14 to -50 with respect transcription start site is critical for cell type-specific MBP gene, which encodes major component sheath in cells derived from central nervous system (CNS). This has ability interact a developmentally controlled DNA-binding mouse brain that stimulates promoter an vitro (Haas, S., J. Gordon, and K. Khalili. 1993. Mol. Cell. Biol. 13:3103-3112). Here, we report purification...
Transcription of the human immunodeficiency virus type-1 (HIV-1) genome is controlled by cooperative interaction viral encoded proteins and host regulatory proteins. In this study, we have examined capacity auxiliary protein, Vpr, to modulate transcriptional activity HIV-1 promoter sequence located within long terminal repeat (LTR). We demonstrate that ectopic expression Vpr in astrocytic cells, U-87MG, enhances basal transfected cells GC-rich sequences, spanning nucleotides −80 −43, are...
ABSTRACT The late region of human neurotropic JC virus encodes a small 71-amino-acid agnoprotein that is also found in the polyomaviruses simian 40 and BK virus. Several functions have been identified, including roles regulating viral transcription virion maturation. Earlier studies showed expressed alone induced p21/WAF-1 expression caused cells to accumulate G 2 /M stage cell cycle. Here we report sensitized cytotoxic effects DNA-damaging agent cisplatin. Agnoprotein reduced viability...
Abstract A family of co‐chaperone proteins that share the Bcl‐2‐associated athanogene (BAG) domain are involved in a number cellular processes, including proliferation and apoptosis. Among these proteins, BAG3 has received increased attention due to its high levels several disease models ability associate with Hsp70 other molecular partners. expression is stimulated during cell response stressful conditions, such as exposure temperature, heavy metals, certain drugs. Here, we demonstrate...