A5001186878- Dermatologic Treatments and Research
- Wound Healing and Treatments
- Salmonella and Campylobacter epidemiology
- Microbial Community Ecology and Physiology
- Burn Injury Management and Outcomes
- Wildlife Ecology and Conservation
- Vibrio bacteria research studies
- Avian ecology and behavior
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Effects of Radiation Exposure
- Biocrusts and Microbial Ecology
- Genital Health and Disease
- Dupuytren's Contracture and Treatments
- Microbial metabolism and enzyme function
- Diabetes Treatment and Management
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Bacterial Infections and Vaccines
- Hair Growth and Disorders
- Skin Protection and Aging
- Cancer Research and Treatments
- Ultrasound and Hyperthermia Applications
- Polar Research and Ecology
- Fermentation and Sensory Analysis
The University of Western Australia
2011-2024
Fiona Wood Foundation
2016-2024
University of Bristol
2023
Intensive Care National Audit & Research Centre
2023
Oxford University Hospitals NHS Trust
2023
University of Ulster
2023
University of Glasgow
1999-2022
National Health and Medical Research Council
2021
Musgrove Park Hospital
2021
Fiona Stanley Hospital
2020
Abstract Archaea and Bacteria constitute a majority of life systems on Earth but have long been considered inferior to Eukarya in terms solute tolerance. Whereas the most halophilic prokaryotes are known for an ability multiply at saturated NaCl (water activity (aw) 0.755) some xerophilic fungi can germinate, usually high-sugar concentrations, values as low 0.650–0.605 aw. Here, we present evidence that prokayotes grow down water activities <0.755 Halanaerobium lacusrosei (0.748),...
ABSTRACT In Escherichia coli , extracytoplasmic stress is partially controlled by the alternative sigma factor, RpoE (ς E ). response to environmental or alteration in protein content of cell envelope, ς upregulates expression a number genes, including htrA . It has been shown that required for intramacrophage survival and virulence Salmonella typhimurium To investigate whether -regulated genes other than are involved salmonella virulence, we inactivated rpoE gene S. SL1344 allelic exchange...
Water availability acts as the most stringent constraint for life on Earth. Thus, understanding water relations of microbial extremophiles is imperative to our ability increase agricultural productivity (e.g., by enhancing processing and turnover dead organic matter in soils arid regions), reduce human exposure mycotoxins buildings food-supply chain, prevent spoilage foods/animal feeds, books, museum specimens artworks better control microbiology industrial fermentations. Only a small number...
Abstract Measures of microbial growth, used as indicators cellular stress, are sometimes quantified at a single time-point. In reality, these measurements compound representations length lag, exponential growth-rate, and other factors. Here, we investigate whether lag phase can act proxy for using number model systems ( Aspergillus penicillioides ; Bacillus subtilis Escherichia coli Eurotium amstelodami , E. echinulatum halophilicum repens; Mrakia frigida Saccharomyces cerevisiae...
An important histological difference between normal, uninjured dermis and scar tissue such as that found in keloid scars is the pattern (morphological architecture) which collagen deposited arranged. In dermis, bundle architecture appears randomly organized (or a basket weave formation), whereas pathological conditions tissue, bundles are often whorls or hypotrophic more densely packed parallel configuration. case of skin, disables leaving it weaker, stiff with loss optimal functionality....
ABSTRACT The CpxAR (Cpx) two-component regulator controls the expression of genes in response to a variety environmental cues. Cpx has been implicated virulence several gram-negative pathogens, although role for vivo not demonstrated directly. Here we investigate whether positive or negative control gene by is important pathogenesis Salmonella enterica serotype Typhimurium. signal pathway Typhimurium was disrupted insertional inactivation cpxA and cpxR genes. We also constitutively activated...
To exploit the microbial ecology of bacterial metabolite production and, specifically, to: (i) evaluate potential use pigments prodigiosin and violacein as additives to commercial sunscreens for protection human skin, (ii) determine antioxidant antimicrobial activities (against pathogenic bacteria) these two pigments.Prodigiosin were used supplement extracts Aloe vera leaf Cucumis sativus (cucumber) fruit which are known have photoprotective activity, well some sunscreen preparations. For...
Actinobacteria perform essential functions within soils, and are dependent on available water to do so. We determined the water-activity (aw ) limits for cell division of Streptomyces albidoflavus, rectiviolaceus, Micromonospora grisea (JCM 3050) over a range temperatures, using culture media supplemented with biologically permissive solute (glycerol). Each species grew optimally at 0.998 aw (control; no added glycerol) growth rates were near-optimal in 0.971-0.974 (1 M temperatures. was...
NaCl-saturated brines such as saltern crystalliser ponds, inland salt lakes, deep-sea and liquids-of-deliquescence on halite are commonly regarded a paradigm for the limit of life Earth. There are, however, other habitats that thermodynamically more extreme. Typically, environments contain all domains perform complete biogeochemical cycling. Despite their reduced water activity, ∼0.755 at 5 M NaCl, some halophiles belonging to Archaea Bacteria exhibit optimum growth/metabolism in these...
Summary For the most‐extreme fungal xerophiles, metabolic activity and cell division typically halts between 0.700 0.640 water (approximately 70.0–64.0% relative humidity). Here, we investigate whether glycerol can enhance xerophile germination under acute water‐activity regimes, using an experimental system which represents biophysical limit of Earth's biosphere. Spores from a variety species, including Aspergillus penicillioides , Eurotium halophilicum Xerochrysium xerophilum (formerly...
Abstract Age is well-known to be a significant factor in both disease pathology and response treatment, yet the molecular changes that occur with age humans remain ill-defined. Here, using transcriptome profiling of healthy human male skin, we demonstrate there period significantly elevated, transcriptome-wide expression occurring predominantly middle age. Both pre post this period, appears undergo much smaller, linear increasing Functional analysis transient suggest heightened metabolic...
A double-blind randomized controlled trial with a paired split-scar design compared verapamil, an L-type Ca2+ channel antagonist, and triamcinolone for prevention of keloid recurrence after excision. blocking activity verapamil in cells was explored. One excised per subject each wound half to receive intralesional injections (10 mg/ml) or (2.5 at monthly intervals (4 doses). Interim analysis performed 14 subjects were completed. Survival demonstrated significantly higher 12 months...
Summary There is a pressing need to understand and optimize biological control so as avoid over‐reliance on the synthetic chemical pesticides that can damage environmental human health. This study focused interactions between novel biocontrol‐strain, Bacillus sp. JC12GB43, potato‐pathogenic Phytophthora Fusarium species. In assays carried out in vitro potato tuber, bacterium was capable of near‐complete inhibition pathogens. sufficiently xerotolerant (water activity limit for growth = 0.928)...
The ability of Salmonella enterica serovar Typhimurium to survive environmental stress requires specific, coordinated, responses, which induce resistance the condition. This study investigated relative contribution sigmaE and sigmaS, sigma factors regulating extracytoplasmic general response functions, respectively, survival at low temperature also in media differing osmotic strength, conditions relevant food preservation. To determine if low-temperature storage is a signal for sigmaE-...
Mycobacteria are associated with a number of well-characterized diseases, yet we know little about their stress biology in natural ecosystems. This study focuses on the isolation and characterization strains from Yellowstone National Park (YNP) Glacier (GNP; USA), majority those identified were Mycobacterium parascrofulaceum, avium or gordonae (GNP). Generally, windows for growth spanned temperature range > 60 °C; selected isolates grew at super-saturated concentrations hydrophobic stressors...
Summary Water activity, temperature and pH are determinants for biotic activity of cellular systems, biosphere function and, indeed, all life processes. This study was carried out at high concentrations glycerol, which concurrently reduces water acts as a stress protectant, to characterize the biophysical capabilities most extremely xerophilic organisms known. These were fungal xerophiles: Xeromyces bisporus ( FRR 0025), Aspergillus penicillioides JH 06 THJ ) Eurotium halophilicum 2471)....
Abstract Scarring is a lifelong consequence of skin injury, with scar stiffness and poor appearance presenting physical psychological barriers to return normal life. Lysyl oxidases are family enzymes that play critical role in formation maintenance. stabilize the main component tissue, collagen, drive appearance. Here we describe development characterisation an irreversible lysyl oxidase inhibitor, PXS-6302. PXS-6302 ideally suited for treatment, readily penetrating when applied as cream...
Objectives: E6 and E7 DNA sequence profile of HPV-18 HPV-45 lineage/sublineage variants in Ghana.Design: A cross-sectional study.Setting: Obstetrics/Gynaecology Directorate, Komfo Anokye Teaching Hospital, Kumasi Department Radio-therapy/Nuclear Medicine the Family Planning Unit, Korle-Bu Accra.Participants: 207 individuals referred with clinical suspicion cervical cancer (CxCa) or confirmed CxCa/precancer cases.Methods: Cervical swabs were collected (from October 2018 to November 2020) from...