A5033348735- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Metal Extraction and Bioleaching
- Mine drainage and remediation techniques
- Arsenic contamination and mitigation
- Trace Elements in Health
- Hydrocarbon exploration and reservoir analysis
- Bacteriophages and microbial interactions
- Chromium effects and bioremediation
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- Aquaculture disease management and microbiota
- Plant Virus Research Studies
- Protist diversity and phylogeny
- Atmospheric and Environmental Gas Dynamics
- Paleontology and Stratigraphy of Fossils
- Marine and coastal ecosystems
- Metabolomics and Mass Spectrometry Studies
- Microbial Fuel Cells and Bioremediation
- Infections and bacterial resistance
- Geology and Paleoclimatology Research
- Polar Research and Ecology
- Salmonella and Campylobacter epidemiology
- Sulfur Compounds in Biology
Bose Institute
2015-2024
University of Burdwan
2009
NaCl-saturated brines such as saltern crystalliser ponds, inland salt lakes, deep-sea and liquids-of-deliquescence on halite are commonly regarded a paradigm for the limit of life Earth. There are, however, other habitats that thermodynamically more extreme. Typically, environments contain all domains perform complete biogeochemical cycling. Despite their reduced water activity, ∼0.755 at 5 M NaCl, some halophiles belonging to Archaea Bacteria exhibit optimum growth/metabolism in these...
Little is known about life in the boron-rich hot springs of Trans-Himalayas. Here, we explore geomicrobiology a 4438-m-high spring which emanates ~70 °C-water from boratic microbialite called Shivlinga. Due to low atmospheric pressure, vent-water close boiling point so can entropically destabilize biomacromolecular systems. Starting vent, Shivlinga's was revealed along thermal gradients an outflow-channel and progressively-drying mineral matrix that has no running water; ecosystem...
The bacterial strain SJTT, along with 15 other mesophilic, neutrophilic and facultatively sulfur-oxidizing chemolithotrophic isolates, was isolated by enrichment on reduced sulfur compounds as the sole energy electron source from soils immediately adjacent to roots of Clitoria ternatea, a slender leguminous herb Lower Gangetic plains India. Strain SJTT able oxidize thiosulfate elemental for chemolithoautotrophic growth. 16S rRNA recA gene sequence-based phylogenetic analyses showed that...
Biogeochemistry of oxygen minimum zone (OMZ) sediments, which are characterized by high input labile organic matter, have crucial bearings on the benthic biota, gas and metal fluxes across sediment-water interface, carbon-sulfur cycling. Here we couple pore-fluid chemistry comprehensive microbial diversity data to reveal sedimentary cycle a water-depth transect covering entire thickness eastern Arabian Sea OMZ, off west coast India. Geochemical show remarkable increase in average total...
To understand SARS-CoV-2 microevolution, this study explored the genome-wide frequency, gene-wise distribution, and molecular nature of all point-mutations detected across its 71,703 RNA-genomes deposited in GISAID till 21 August 2020. Globally, nsp1/nsp2 orf7a/orf3a were most mutation-ridden non-structural structural genes respectively. Phylogeny 4618 spatiotemporally-representative genomes revealed that entities belonging to early lineages are mostly spread over Asian countries, including...
Twelve chemolithotrophic strains were isolated from temperate orchard soil on reduced sulfur compounds as energy and electron sources characterized the basis of their physiological properties ability to oxidize various compounds. The new isolates could tetrathionate well thiosulfate, oxidation latter involved conversion thiosulfate followed by its accumulation eventual sulfate, manifested in production acid. mesophilic, neutrophilic, Gram-negative coccoid bacteria had a respiratory...
The SoxXAYZB(CD)2 -mediated pathway of bacterial sulfur-chemolithotrophy explains the oxidation thiosulfate, sulfide, sulfur and sulfite but not tetrathionate. Advenella kashmirensis, which oxidizes tetrathionate to sulfate, besides forming it as an intermediate during thiosulfate oxidation, possesses a soxCDYZAXOB operon. Knock-out mutations proved that only SoxBCD is involved in A. kashmirensis whereas thiosulfate-to-tetrathionate conversion Sox independent. Expression two glutathione...
ABSTRACT The ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal bacterial communities along ∼3 m sediment-horizons the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath sea-level) and 580 were at 15–30 cm intervals, using metagenomics, pure-culture-isolation, genomics metatranscriptomics. Genes for respiration, oxidation...
The chemolithoautotrophic betaproteobacterium Tetrathiobacter kashmirensis belongs to the family Alcaligenaceae and is phylogenetically closely related pathogens such as Taylorella Bordetella species. While a complete inorganic sulfur oxidation gene cluster, soxCDYZAXWB, present in its genome, pathogenicity islands or genes associated with virulence, disease, cellular invasion, and/or intracellular resistance are completely absent.
During chemolithoautotrophic thiosulfate oxidation, the phylogenetically diverged proteobacteria Paracoccus pantotrophus , Tetrathiobacter kashmirensis and Thiomicrospira crunogena rendered steady enrichment of 34 S in end product sulfate, with overall fractionation ranging between −4.6‰ +5.8‰. The kinetics T. was essentially similar to that P. albeit former had a slightly higher magnitude rate enrichment. In case only significant departure its curve from observed during first 36 h...
Abstract To elucidate how geothermal irregularities affect the sustainability of high-temperature microbiomes we studied synecological dynamics a microbial mat community (GMMC) vis-à-vis fluctuations in its environment. Spatiotemporally-discrete editions photosynthetic GMMC colonizing travertine mound circum-neutral hot spring cluster served as model-system. In 2010 strong geyser atop discharged mineral-rich water, which nourished continuum from proximal channels (PC) upto slope environment...
A Gram-stain-positive, spore-forming bacterium, EAG3T, capable of growing on 3-nitropropionic acid as the sole source carbon, nitrogen and energy, was isolated from anterior gut an earthworm (Eisenia fetida) reared at Centre Floriculture Agribusiness Management University North Bengal Siliguri (26.7072° N, 88.3558° E), West Bengal, India. The DNA G+C content strain EAG3T 42.5 mol%. Strain contained MK-7 MK-8 predominant menaquinones. polar lipids were phosphatidylglycerol,...
Abstract. To explore the potential role of tetrathionate in sedimentary sulfur cycle, population ecology microorganisms capable metabolizing this polythionate was revealed at 15–30 cm resolution along two, ∼3 m long, cores collected from 530 and 580 below sea level, off India's west coast, within oxygen minimum zone (OMZ) Arabian Sea. Metagenome analysis widespread occurrence genes involved formation, oxidation, reduction tetrathionate; high diversity relative abundance were also detected...
Two tropical leguminous-rhizospheric strains, SST and JT 001, phylogenetically closest to Paracoccus thiocyanatus pantotrophus, respectively, were isolated on reduced sulfur compounds as sole energy electron sources. While had versatile chemolithotrophic abilities oxidize thiosulfate, tetrathionate, thiocyanate, sulfide elemental sulfur, 001 could soluble sulfide, a relatively lesser amount of tetrathionate. Positive hybridization signals detected for but not SST, when their genomic DNAs...
Abstract Silica and travertine deposits, the two most common surface manifestations of terrestrial hot springs, have so far been only markers universally helpful in locating interpreting geothermal systems past lifeforms potentially associated with them. In current study, we for first time report microbial fossils from a third type sinter, viz. boron mineral which are characteristic relatively uncommon silicate-poor springs. Organic biomarker analyses boratic sinters framing springs Puga...
ABSTRACT Tetrathiobacter spp. and other members of the Alcaligenaceae are metabolically versatile environmentally significant. A novel, ∼60-kb conjugative plasmid, pBTK445, from sulfur chemolithoautotroph kashmirensis , was identified characterized. This plasmid exists at a low copy number 2 to 3 per host chromosome. The portion pBTK445 sequenced so far (∼25 kb) harbors genes putatively involved in replication, transfer functions, partition, UV damage repair. 1,373-bp region as minimal...