A5051870360- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Monoclonal and Polyclonal Antibodies Research
- Acute Myeloid Leukemia Research
- Peroxisome Proliferator-Activated Receptors
- Pelvic floor disorders treatments
- Receptor Mechanisms and Signaling
- Congenital heart defects research
- Glycosylation and Glycoproteins Research
- Alcohol Consumption and Health Effects
- Developmental Biology and Gene Regulation
- Fatty Acid Research and Health
- Urinary Tract Infections Management
- MicroRNA in disease regulation
- Herpesvirus Infections and Treatments
- Protein Degradation and Inhibitors
- Healthcare Systems and Public Health
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer Risks and Factors
- Metabolism, Diabetes, and Cancer
- Histone Deacetylase Inhibitors Research
- Steroid Chemistry and Biochemistry
- Asthma and respiratory diseases
TriHealth
2022-2025
Cincinnati Children's Hospital Medical Center
2022-2024
Australia and New Zealand Banking Group
2023-2024
University of Michigan
2016-2023
NewYork–Presbyterian Brooklyn Methodist Hospital
2022-2023
Robert Bosch (Germany)
2023
University of South Carolina
2022
Thomas Jefferson University
2011-2016
University of California, Santa Cruz
2016
Centre National de la Recherche Scientifique
2013
Circulating platelets roll along exposed collagen at vessel injury sites and respond with filipodia protrusion, shape change, surface area expansion to facilitate platelet adhesion plug formation. Various glycoproteins were considered be both responders mediators of adhesion, yet the signaling kinetics emanating from these receptors do not fully account for rapid cytoskeletal changes that occur in blood flow. We found free N-terminal fragment G protein-coupled receptor (GPCR) GPR56 human...
Mammalian Argonaute 2 (Ago2) protein associates with microRNAs (miRNAs) or small interfering RNAs (siRNAs) forming RNA-induced silencing complexes (RISCs/miRNPs). In the present work, we characterize RNA-binding and nucleolytic activity of recombinant mouse Ago2. Our studies show that Ago2 binds efficiently to miRNAs active RISC. Surprisingly, find forms RISC using pre-miRNAs long unstructured single stranded as guides. Furthermore, demonstrate that, in vivo , endogenous human directly...
Human platelet-type 12-lipoxygenase (12-LOX) has recently been shown to play an important role in regulation of human platelet function by reacting with arachidonic acid (AA). However, a number other fatty acids are present on the surface that, when cleaved from phospholipid, can be oxidized 12-LOX. We sought characterize substrate specificity 12-LOX against six essential acids: AA, dihomo-γ-linolenic (DGLA), eicosapentaenoic (EPA), α-linolenic (ALA), eicosadienoic (EDA), and linoleic (LA)....
Human lipoxygenases (LOXs) are a family of iron-containing enzymes which catalyze the oxidation polyunsaturated fatty acids to provide corresponding bioactive hydroxyeicosatetraenoic acid (HETE) metabolites. These eicosanoid signaling molecules involved in number physiologic responses such as platelet aggregation, inflammation, and cell proliferation. Our group has taken particular interest platelet-type 12-(S)-LOX (12-LOX) because its demonstrated role skin diseases, diabetes, hemostasis,...
Dietary supplementation with polyunsaturated fatty acids has been widely used for primary and secondary prevention of cardiovascular disease in individuals at risk; however, the cardioprotective benefits remain controversial because lack mechanistic vivo evidence. We present direct evidence that an omega-6 acid, dihomo-γ-linolenic acid (DGLA), exhibits cardioprotection through 12-lipoxygenase (12-LOX) oxidation DGLA to its reduced oxidized lipid form, 12(S)-hydroxy-8Z,10E,14Z-eicosatrienoic...
Dysregulation of innate immune signaling is a hallmark hematologic malignancies. Recent therapeutic efforts to subvert aberrant in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) have focused on the kinase IRAK4. IRAK4 inhibitors achieved promising, though moderate, responses preclinical studies clinical trials for MDS AML. The reasons underlying limited remain unknown. In this study, we reveal that inhibiting leukemic cells elicits functional complementation compensation by...
This study is important as it challenges the effectiveness of silver-coated catheters in reducing urinary tract infections (UTIs) after pelvic floor surgery (PFS).
Objective— Platelet activation after stimulation of PAR (protease-activated receptor) 4 is heightened in platelets from blacks compared with those whites. The difference PAR4 signaling by race partially explained a single-nucleotide variant encoding for either an alanine or threonine at amino acid 120 the second transmembrane domain. current study sought to determine whether this because biased G q and activity results resistance traditional antiplatelet intervention. Approach Results—...
A protein modifying agent, p-nitrobenzenesulfonyl fluoride, has been shown to be specific for tyrosine.The reaction conditions are mild (in aqueous buffers at 25 "C), and the rate increases with pH.The product is a derivative of 0-sulfonylated tyrosine which yields 0-(p-nitrobenzenesulfonyl) (NBS-Tyr) after acid hydrolysis.The extent modification can measured as NBS-Tyr by high performance liquid chromatography (HPLC).The reagent was used study reactivity importance in enzymic function...
The antiplatelet effect of polyunsaturated fatty acids is primarily attributed to its metabolism bioactive metabolites by oxygenases, such as lipoxygenases (LOX). Platelets have demonstrated the ability generate 15-LOX-derived (15-oxylipins); however, whether 15-LOX in platelet or required for formation 15-oxylipins remains unclear. This study seeks elucidate and determine their mechanistic effects on reactivity. In this study, 15-HETrE, 15-HETE, 15-HEPE attenuated collagen-induced...
The limb- and heart-specific Tbx5 transcription factor coexpresses with directly binds to the novel PDZ-LIM domain protein, LMP4. LMP4 is distributed in cytoplasm associated actin cytoskeleton. In presence of LMP4, shuttles dynamically between nucleus and, a complex localizes filaments. Nuclear cytoplasmic distribution developing chicken wings suggests functional significance LMP4–Tbx5 interaction. primary epicardial cells, we demonstrate that protein subcellular relocalization can be...
Abstract Arterial thrombosis is the underlying cause for a number of cardiovascular-related events. Although dietary supplementation that includes polyunsaturated fatty acids (PUFAs) has been proposed to elicit cardiovascular protection, mechanism antithrombotic protection not well established. The current study sought investigate whether an omega-6 essential acid, docosapentaenoic acid (DPAn-6), and its oxidized lipid metabolites (oxylipins) provide direct through inhibition platelet...
We report a case of varicella-zoster virus (VZV) myelitis in woman with relapsing-remitting multiple sclerosis (RRMS) receiving natalizumab, humanized monoclonal antibody that induces an immunosuppression localized to the CNS.