A5061938649- Pain Mechanisms and Treatments
- Epigenetics and DNA Methylation
- Neurogenesis and neuroplasticity mechanisms
- Hedgehog Signaling Pathway Studies
- Genetics and Neurodevelopmental Disorders
- Pharmacological Receptor Mechanisms and Effects
- Microtubule and mitosis dynamics
- Pharmacological Effects and Toxicity Studies
- Pharmacological Effects of Natural Compounds
- Neuroscience and Neuropharmacology Research
- Glioma Diagnosis and Treatment
- Epilepsy research and treatment
- Receptor Mechanisms and Signaling
- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Anesthesia and Pain Management
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- RNA Research and Splicing
- Cannabis and Cannabinoid Research
- Nitric Oxide and Endothelin Effects
- Phenothiazines and Benzothiazines Synthesis and Activities
- Cancer, Stress, Anesthesia, and Immune Response
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Université Libre de Bruxelles
2012-2023
VIB-KU Leuven Center for Brain & Disease Research
2018-2022
KU Leuven
2019
University of Geneva
2008-2010
Inserm
2003-2008
Clermont Université
2008
Université Clermont Auvergne
2006
Fondation FondaMental
2005
Marymount University
2003
Acetaminophen is the most used analgesic/antipyretic drug. Its unclear mechanism of action could rely on cyclooxygenase inhibition, NO synthesis blockade or reinforcement serotonergic system. Here we show that in thermal, mechanical and chemical pain tests, AM-251, a specific CB1 receptor antagonist, abolished analgesic acetaminophen, which was also lost knockout mice. Moreover, acetaminophen shown unable to bind receptors demonstrating an indirect involvement these effect this compound....
The primary cilium is a central signaling component during embryonic development. Here we focus on CROCCP2, hominid-specific gene duplicate from ciliary rootlet coiled coil (CROCC), also known as rootletin, that encodes the major of rootlet. We find CROCCP2 highly expressed in human fetal brain and not other primate species. gain function mouse cortex cortical cells organoids results decreased ciliogenesis increased progenitor amplification, particularly basal progenitors. decreases dynamics...
The chemokine receptor CCR5 is well known for its function in immune cells; however, it also expressed the brain, where specific role remains to be elucidated. Because genetic factors may influence risk of developing cerebral ischaemia or affect clinical outcome, we have analysed experimental stroke.Permanent was performed by occlusion middle artery wild-type and CCR5-deficient mice. Locomotor behaviour, infarct size histochemical alterations were at different time points after occlusion.The...
During neurogenesis, progenitors switch from self-renewal to differentiation through the interplay of intrinsic and extrinsic cues, but how these are integrated remains poorly understood. Here, we combine whole-genome transcriptional epigenetic analyses with in vivo functional studies demonstrate that Bcl6, a repressor previously reported promote cortical acts as driver neurogenic transition direct silencing selective repertoire genes belonging multiple pathways promoting self-renewal, most...
The regulation of nociceptive processing by 5-HT at the spinal level is intricate since neurotransmitter has been implicated in both pro and antinociception. aim our study was to investigate, according nature noxious stimulus, how blockade 5-HT(1A) receptors could influence antinociceptive actions exogenous as well two analgesics involving endogenous 5-HT, paracetamol venlafaxine. Rats were submitted either formalin test (tonic pain) or paw pressure (acute pain). WAY-100635 (40 microg/rat,...
NOX enzymes are reactive oxygen species (ROS)-generating NADPH oxidases. Several members of the family depend on p22(phox) subunit, encoded by CYBA gene. is highly polymorphic, and has been widely studied as a potential risk factor for various diseases, with conflicting results. In present study, we used Epstein-Barr (EBV)-transformed B-lymphocytes from 50 healthy unrelated individuals to analyze their mRNA sequence NOX2-dependent ROS generation. Seven single-nucleotide polymorphisms (SNPs)...
Background The mechanism of action paracetamol (acetaminophen) remains elusive because it is still under discussion as to whether acts locally and/or centrally. primary aim this study was clarify its site(s) (central local) using the rat formalin test. Methods Spontaneous biting and licking injected paw following intraplantar injection 2.5% monitored during two phases nociceptive behavior (0-5 20-40 min after injection), authors examined antinociceptive activity oral, intravenous,...
The reversal of the antinociceptive effect systemically administered acetaminophen (paracetamol) by intrathecal administration potent 5-HT(3) receptor antagonist tropisetron has been reported in rats subjected to paw pressure test, suggesting that action is mediated through spinal receptors. However, more recent data, showing differences between pharmacological profiles various antagonists, led us reconsider involvement To address this question, two different approaches were used: 1)...
The mechanism of action acetaminophen is currently widely discussed. Direct inhibition cyclooxygenase isoforms remains the commonly advanced hypothesis. We combined behavioral studies with molecular techniques to investigate in a model tonic pain rats. show that indirectly stimulates spinal 5-hydroxytryptamine (5-HT)<sub>1A</sub> receptors formalin test, thereby increasing transcript and protein levels low-affinity neurotrophin receptor, insulin-like growth factor-1 (IGF-1) receptor α...
The risk of developing drug addiction is strongly influenced by the epigenetic landscape and chromatin remodeling. While histone modifications such as methylation acetylation have been studied in ventral tegmental area nucleus accumbens (NAc), role H2A monoubiquitination remains unknown. Our investigations, initially focused on scaffold protein melanoma-associated antigen D1 (Maged1), reveal that paraventricular thalamus (PVT) significantly contributes to cocaine-adaptive behaviors...
Summary The primary cilum is a central component of signalling during neural development, from regional patterning to neuronal differentiation. Here we focus on CROCCP2, hominid-specific gene duplicate CROCC (Ciliary Rootlet Coiled Coil), also known as rootletin, that encodes the major protein ciliary rootlet. We find CROCCP2 highly expressed in human fetal brain and not other primate species. gain function mouse embryonic cortex results decreased ciliogenesis, increased mTOR signalling,...