A5027244059- Protein Structure and Dynamics
- Crystallization and Solubility Studies
- Glycosylation and Glycoproteins Research
- X-ray Diffraction in Crystallography
- Chemokine receptors and signaling
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Algal biology and biofuel production
- Biotin and Related Studies
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Computational Drug Discovery Methods
- T-cell and B-cell Immunology
- Bacterial Genetics and Biotechnology
- Click Chemistry and Applications
- Bioinformatics and Genomic Networks
- Bone Tissue Engineering Materials
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Immune Response and Inflammation
- Microbial Metabolic Engineering and Bioproduction
- Aquatic Ecosystems and Phytoplankton Dynamics
- 14-3-3 protein interactions
- Helicobacter pylori-related gastroenterology studies
Indian Institute of Technology Roorkee
2016-2025
L V Prasad Eye Institute
2023-2024
Manav Rachna International Institute of Research and Studies
2014-2023
Harnessing the halotolerant characteristics of microalgae provides a viable alternative for sustainable biomass and triacylglyceride (TAG) production. Scenedesmus sp. IITRIND2 is fast growing fresh water microalga that has capability to thrive in high saline environments. To understand microalga's adaptability, we studied its physiological metabolic flexibility by studying differential protein, metabolite lipid expression profiles using metabolomics, proteomics, real-time polymerase chain...
Prerequisite for structural studies on G protein-coupled receptors is the preparation of highly concentrated, stable, and biologically active receptor samples in milligram amounts protein. Here, we present an improved protocol E. coli expression, functional refolding reconstitution into bicelles human neuropeptide Y type 2 (Y2R) solution solid-state NMR experiments. The isotopically labeled expressed inclusion bodies purified using SDS. We studied details including vitro folding receptor,...
The therapeutic use of bacteriophage-encoded endolysins as enzybiotics has increased significantly in recent years due to the emergence antibiotic resistant bacteria. Phage lyse bacteria by targeting their cell wall. Various engineering strategies are commonly used modulate or enhance utility enzymes. This study employed a structure-guided mutagenesis approach engineer T7 bacteriophage endolysin (T7L) with enhanced amidase activity and lysis potency via replacement noncatalytic gating...
Chemokines are chemotactic cytokines involved in leucocyte trafficking to infected tissue. Growth-related oncogene (GRO) chemokines namely CXCL1, CXCL2 and CXCL3 neutrophil activating sharing a conserved three-dimensional structure, but encompassing functional diversity due gene duplication evolutionary events. However, the mechanisms including selection pressures diversification of GRO genes have not yet been characterized. Here, we performed comprehensive analysis among different mammalian...
Retinoblastoma (Rb) is a pediatric eye cancer which if diagnosed at later stages can lead to Rb invasion into the choroid, optic nerve, sclera, or beyond, with potential of undergoing metastasis. Cancer cells, including reprogram their metabolic circuits for own survival and progression, provides great opportunity monitor extent progression based on differences. Henceforth, present study aims map variations in patients invasive (primarily enucleated eyes high-risk histopathological features)...
Retinoblastoma (Rb) is the most common pediatric eye cancer. To identify biomarkers for early diagnosis and monitoring progression of Rb in patients, mapping alterations their metabolic profiles essential. The present study aims at exploring disparity serum from patients controls using NMR-based metabolomics. A total 72 metabolites, including carbohydrates, amino acids, organic were quantified samples 24 26 controls. Distinct clusters obtained partial least-squares discriminant analysis...