A5085850043- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Renal and related cancers
- Prostate Cancer Diagnosis and Treatment
- Immune cells in cancer
- Genomics and Chromatin Dynamics
- NF-κB Signaling Pathways
- Radiopharmaceutical Chemistry and Applications
- Bladder and Urothelial Cancer Treatments
- Chromatin Remodeling and Cancer
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Urologic and reproductive health conditions
- Genetics and Neurodevelopmental Disorders
- Molecular Biology Techniques and Applications
- Sarcoma Diagnosis and Treatment
- Urinary Bladder and Prostate Research
- RNA Interference and Gene Delivery
- Pancreatic function and diabetes
- Telomeres, Telomerase, and Senescence
- Lysosomal Storage Disorders Research
- interferon and immune responses
The University of Tokyo
2021-2024
Systems Biology Institute
2019-2022
University of California, Los Angeles
2019-2021
QB3
2019
Kyoto University
2013-2015
Sanno Medical Center
2008
Tenri Hospital
1999-2003
National Center of Neurology and Psychiatry
2003
Hamamatsu Medical Center
2003
Fukuda Denshi (Japan)
2003
Timing cues epigenomic reprogramming Different temporal dynamics of activation the transcription factor nuclear κB (NF-κB) can influence inflammatory response activated macrophages. Cheng et al. report a mechanism by which oscillatory and sustained NF-κB signaling may produce distinct transcriptional responses (see Perspective Nandagopal ). Oscillatory poised enhancers to transcribe genes in mouse However, produced cells other stimuli acted on epigenome. These relieved chromatin silencing at...
Abstract De novo establishment of DNA methylation is accomplished by DNMT3A and DNMT3B. Here, we analyze de in mouse embryonic fibroblasts (2i-MEFs) derived from DNA-hypomethylated 2i/L ES cells with genetic ablation Dnmt3a or Dnmt3b . We identify 355 333 uniquely unmethylated genes knockout (KO) 2i-MEFs, respectively. find that exclusively required for at both TSS regions gene bodies Polycomb group (PcG) target developmental genes, while has a dominant role on the X chromosome. Consistent...
Alternative splicing generates multiple transcripts from a single gene, and cell-type-specific profiles are important for the properties functions of cells. Recently, somatic cells have been shown to undergo dedifferentiation after forced expression transcription factors. However, it remains unclear whether cell is reorganized during reprogramming. Here, by combining deep sequencing with high-throughput absolute qRT-PCR, we show that revert pluripotent ones Remarkably, pattern in stem...
Abstract In vivo reprogramming provokes a wide range of cell fate conversion. Here, we discover that in induction higher levels OSKM mouse somatic cells leads to increased expression primordial germ (PGC)-related genes and genome-wide erasure genomic imprinting, which takes place exclusively PGCs. Moreover, the results development cancer resembles human tumors. Like subgroup tumors, propagated tumor can differentiate into trophoblasts. these give rise induced pluripotent stem (iPSCs) with...
Atypical teratoid/rhabdoid tumor (AT/RT), which harbors SMARCB1 mutation and exhibits a characteristic histology of rhabdoid cells, has poor prognosis because the lack effective treatments. Here, we establish human SMARCB1-deficient pluripotent stem cells (hPSCs). hPSC-derived neural progenitor-like (NPLCs) efficiently give rise to brain tumors when transplanted into mouse brain. Notably, activation an embryonic cell (ESC)-like signature confers in NPLC-derived causes prognosis....
Abstract Macrophages orchestrate immune responses by sensing and responding to pathogen-associated molecules. These are modulated prior conditioning with cytokines such as interferons (IFNs). Type I II IFN have opposing functions in many biological scenarios, yet macrophages directly stimulated or activate highly overlapping gene expression programs. We hypothesized that a sequential conditioning-stimulation approach would reveal greater specificity the differential effects of on human...
Abstract Clear cell sarcoma (CCS) is a rare soft tissue caused by the EWS/ATF1 fusion gene. Here, we established induced pluripotent stem cells (iPSCs) from -controllable murine CCS harboring sarcoma-associated genetic abnormalities. Sarcoma-iPSC mice develop secondary sarcomas immediately after induction, but only in tissue. expression induces oncogene-induced senescence most types sarcoma-iPSC prevents it cells. We identify Tppp3 -expressing peripheral nerves as cell-of-origin for these...
CpG islands (CGIs) including those at imprinting control regions (ICRs) are protected from de novo methylation in somatic cells. However, many cancers often exhibit CGI hypermethylation, implying that the machinery is impaired cancer Here, we conducted a comprehensive analysis of during cell reprogramming. Although most CGIs remain hypomethylated, small subset CGIs, particularly several ICRs, was methylated reprogrammed pluripotent stem cells (PSCs). Such ICR linked with silencing...
The resistance to transcription factor-mediated reprogramming into pluripotent stem cells is one of the distinctive features cancer cells. Here we dissect profiles factor binding and subsequent transcriptional response in reveal its underlying mechanisms. Using clear cell sarcomas (CCSs), show that driver oncogene EWS/ATF1 misdirects factors cancer-specific enhancers thereby impairs toward pluripotency otherwise provoked. Sensitization cue observed other types when corresponding oncogenic...
We report of an infant with neonatal glycogen storage disease type IV (GSD IV) who was examined for severe hypotonia and cardiomyopathy. On the muscle biopsy there were many fibers diastase-resistant polyglucosan bodies. Glycogen branching enzyme (GBE1) activity in markedly reduced. The had a homozygous single nucleotide deletion open reading frame GBE1 gene.
Allelotype analyses of human prostate cancer indicate that allelic losses on chromosome arms 7q, 8p, 10q, 13q, 16q, 17q, and 18q are observed frequently. For the study possible biological significance frequently deletions arm 7q in cancer, 7 was introduced into highly metastatic rat cells by use a microcell-mediated transfer technique. The introduction resulted suppression ability microcell hybrids, whereas no tumorigenicity observed. To identify portion containing metastasis-suppressive...
It remains controversial whether the routes from somatic cells to induced pluripotent stem (iPSCs) are related reverse order of normal developmental processes. Specifically, it unaddressed or not differentiated become iPSCs through their original tissue cell-like state. Previous studies analyzing reprogramming process mostly used fibroblasts; however, cell characteristics fibroblasts made difficult address this. Here, we generated mouse astrocytes, a type glial cells, by three (OCT3/4, KLF4,...
No AccessJournal of UrologyCLINICAL UROLOGY: Case Reports1 Jul 2001HIGH DOSE HOOK EFFECT IN SERUM TOTAL AND FREE PROSTATE SPECIFIC ANTIGEN A PATIENT WITH METASTATIC CANCER YUZO FURUYA, SHUKO CHO, SHO OHTA, NAOHIDE SATO, TADASHI KOTAKE, and MOTOYUKI MASAI FURUYAYUZO FURUYA More articles by this author , CHOSHUKO CHO OHTASHO OHTA SATONAOHIDE SATO KOTAKETADASHI KOTAKE MASAIMOTOYUKI View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)66117-4AboutFull TextPDF ToolsAdd to...
Testicular involvement by sarcoidosis is a rare condition. A 23-year-old Japanese man had asymptomatic bilateral testicular lesions, which were detected gallium scintigram, together with lesions located bilaterally in the uvea, lungs and hilar, mediastinal lymph nodes unilateral supraclavicular nodes. Semen analysis demonstrated severely impaired spermatogenesis. Treatment corticosteroid dramatically improved these restored This case report suggests that may cause male infertility.
PTBP1, a well-conserved RNA-binding protein, regulates cellular development by tuning posttranscriptional mRNA modification such as alternative splicing (AS) or stabilization. We previously revealed that the loss of Ptbp1 in spermatogonia causes dysregulation spermatogenesis, but molecular mechanisms which PTBP1 spermatogonium homeostasis are unclear. In this study, changes AS transcriptome Ptbp1-knockout (KO) germline stem cells (GSC), an vitro model proliferating spermatogonia, was...