A5060527731- Blood properties and coagulation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Osteoarthritis Treatment and Mechanisms
- Tendon Structure and Treatment
- Cellular Mechanics and Interactions
- Venous Thromboembolism Diagnosis and Management
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Nanoparticle-Based Drug Delivery
- Blood Coagulation and Thrombosis Mechanisms
- Silk-based biomaterials and applications
- Acute Ischemic Stroke Management
- Proteoglycans and glycosaminoglycans research
- Skin Protection and Aging
- Periodontal Regeneration and Treatments
- Infrared Thermography in Medicine
- Hydrogels: synthesis, properties, applications
- Coronary Interventions and Diagnostics
- Photochromic and Fluorescence Chemistry
- Connective tissue disorders research
- Hemophilia Treatment and Research
- Lung Cancer Treatments and Mutations
- Click Chemistry and Applications
- Graphene and Nanomaterials Applications
- Electrospun Nanofibers in Biomedical Applications
Purdue University West Lafayette
2020-2024
Massachusetts General Hospital
2023-2024
Sun Yat-sen University
2024
Indiana University – Purdue University Indianapolis
2020-2024
Indiana University School of Medicine
2020-2024
Harvard University
2023-2024
Peking University
2020-2023
King University
2021
Miami University
2017-2021
University School
2020
Immune checkpoint blockades (ICBs) are a promising treatment for cancers such as melanoma by blocking important inhibitory pathways that enable tumor cells to evade immune attack. Programmed death ligand 1 monoclonal antibodies (aPDL1s) can be used an ICB significantly enhance the effectiveness of immunotherapy PD-1/PD-L1 pathway. However, aPDL1s may limited low selectivity in vivo and immunosuppressed microenvironment including hypoxia.To overcome limitations, we develop multifunctional...
Viscoelastic transformation of tissue drives aberrant cellular functions and is an early biomarker disease pathogenesis. Tissues scale a range viscoelastic moduli, from biofluids to bone. Moreover, behavior governed by the frequency at which probed, yielding distinct viscous elastic responses modulated over wide band. Existing tools do not quantify wideband spectra in tissues, leaving vast knowledge gap. We present laser speckle rheological microscopy (WB-SHEAR) that reveals response...
A great need exists for the development of a more representative in-vitro model to efficiently screen novel thrombolytic therapies. We herein report design, validation, and characterization highly reproducible, physiological scale, flowing clot lysis platform with real-time fibrinolysis monitoring drugs utilizing fluorescein isothiocyanate (FITC)-labeled analog. Using this Real-Time Fluorometric Flowing Fibrinolysis assay (RT-FluFF assay), tPa-dependent degree thrombolysis was observed both...
Osteochondral regeneration remains a key challenge because of the limited self-healing ability bone and its complex structure composition. Biomaterials based on endochondral ossification (ECO) are considered an attractive candidate to promote repair they can effectively address difficulties in establishing vascularization poor via intramembranous (IMO). However, clinical application is by cellular behavior ECO long time required for induction cell cycle. Herein, functionalized...
Thrombosis can lead to a wide variety of life-threatening circumstances. As current thrombolytic drug screening models often poorly predict profiles, leading failure therapy or clinical translation, more representative clot substrates are necessary for evaluation. Utilizing Chandler loop device form analogs at high shear has gained popularity in stroke societies. However, shear-dependent microstructure not been fully addressed and low conditions overlooked. We herein characterized the impact...
Neurovascularized bone regeneration remains an enormous challenge in the clinic.
A tripeptide-based macroporous hydrogel simultaneously promoted osteogenesis and angiogenesis, which is significant for 3D cultures stem cell-based tissue engineering.
Using injectable hydrogels loaded with mesenchymal stem cells (MSCs) to repair chondral defects is a new trend of cartilage tissue engineering in recent years. In this study, hyaluronic acid (HA) containing the system sustained-release Kartogenin (KGN) and modified by RGD HAV peptides were used facilitate defect knee joint rabbits. Different groups implants injected into osteochondral defects, samples taken 4 weeks after operation. Through qualitative quantitative analysis Micro-CT, it can...
Conducting in-vitro thrombosis research presents numerous challenges, the primary of which is working with blood products, whether whole or fractionated blood, that have limited functional shelf-lives. As a result, being able to significantly prolong clotting functionality via fractionation and recombination promises greater accessibility resource minimization in realm research. Whole CPDA1 from healthy volunteers was stored as frozen platelet-free plasma (PFP, -20°C), refrigerated packed...
Real-time fibrinolytic testing leveraging a physiologically relevant labeled fibrin clot substrate in unique multiplexed annular geometry.
The viscoelastic properties and microstructure of the fibrin clot network are compromised in patients with coagulation abnormalities. In this study, we harness two novel optical approaches, iCoagLab Spectrally Encoded Confocal Microscopy (SECM), to investigate how modifications blood clots reflected microstructural features coagulopathic patients. We observe that viscoelasticity is directly related density, fiber length, straightness a fully polymerized network.
Abstract Studies aiming to understand the effects of storage on whole blood (WB) clotting often rely characterizing coagulation under static conditions. Minimal work has explored physiologic shear clot formation and thrombolysis utilizing fractionated reconstituted (rWB) products. WB was into platelet‐free plasma, packed red cells, platelets storing each component its ideal conditions—including platelet cryopreservation. Recombination at their native ratios accomplished over 91 days...
Particle sizing of biomaterials and tissues holds profound implications across diverse industrial clinical disciplines. Conventional approaches such as dynamic light scattering (DLS) are restricted to dilute liquids, unable assess particle size distribution in intact, untampered their native state. Here, we introduce the laser Speckle PARticle SizEr (SPARSE), which leverages size-dependent attributes polarized back-scattered speckle characterize average radius a continuum endogenous...
Abstract Mechanical transformation of tissue is not merely a symptom but decisive driver in pathological processes. Comprising intricate network cells, fibrillar proteins, and interstitial fluid, tissues exhibit distinct solid- (elastic) liquid-like (viscous) behaviours that span wide band frequencies. Yet, characterization wideband viscoelastic behaviour whole has been investigated, leaving vast knowledge gap the higher frequency range linked to fundamental intracellular processes...
Metabolic dysfunction of β-cells has been implicated as a contributor to diabetes pathogenesis, and efforts are ongoing optimize analytical techniques that evaluate islet metabolism. High-resolution respirometry offers sensitive measurements the respiratory effects metabolic substrates customizable manipulation electron transport chain components, though delicate nature islets can pose challenges conventional analyses. An affordable reliable option for is Oroboros Oxygraph-2 K system, which...
Thrombosis is a leading cause of death worldwide. Fibrin(ogen) the protein primarily responsible for clot formation or thrombosis. Therefore, characterizing fibrin beneficial to study Turbidity and thromboelastography (TEG) are both widely utilized in vitro assays monitoring formation. dynamically measures light transmittance through structure via spectrometer often used research laboratories. TEG specialized viscoelastic technique that directly blood strength clinical settings assess...
There is an emerging interest in utilizing synthetic multivalent inhibitors that comprise of multiple inhibitor moieties linked on a common scaffold to achieve strong and selective enzyme inhibition. As inhibition impacted by valency linker length, this study, we explore the effect benzamidine varying length plasmin Plasmin endogenous responsible for digesting fibrin present blood clots. Monovalent plasmin(ogen) are utilized clinically treat hyperfibrinolysis-associated bleeding events....