A5015679628- interferon and immune responses
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Herpesvirus Infections and Treatments
- Innovative Teaching Methods
- Tryptophan and brain disorders
- Genetics, Bioinformatics, and Biomedical Research
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cytomegalovirus and herpesvirus research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- NF-κB Signaling Pathways
- RNA regulation and disease
- Virus-based gene therapy research
- Systemic Lupus Erythematosus Research
- Viral Infectious Diseases and Gene Expression in Insects
- Radiopharmaceutical Chemistry and Applications
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- Biomedical and Engineering Education
- Asthma and respiratory diseases
- Renal cell carcinoma treatment
- Advanced Fluorescence Microscopy Techniques
University of California, Los Angeles
2019-2023
QB3
2019-2023
Quantitative BioSciences
2022
University Hospital Schleswig-Holstein
2017-2021
University of Lübeck
2017-2021
Kiel University
2015-2021
Aarhus University
2016-2021
Utrecht University
2015
Varicella zoster virus (VZV) typically causes chickenpox upon primary infection. In rare cases, VZV can give rise to life-threatening disease in otherwise healthy people, but the immunological basis for this remains unexplained. We report 4 cases of acute severe infection affecting central nervous system or lungs unrelated, children who are heterozygous missense mutations POLR3A (one patient), POLR3C both (two patients). and encode subunits RNA polymerase III. Leukocytes from all patients...
Macrophages initiate inflammatory responses via the transcription factor NFκB. The temporal pattern of NFκB activity determines which genes are expressed and thus, type response that ensues. Here, we examined how information about stimulus is encoded in dynamics activity. We generated an mVenus-RelA reporter mouse line to enable high-throughput live-cell analysis primary macrophages responding host- pathogen-derived stimuli. An information-theoretic workflow identified six dynamical...
The interaction of the MHC class I-related chain molecules A and B (MICA MICB) UL-16 binding protein (ULBP) family members expressed on tumor cells with corresponding NKG2D receptor triggers cytotoxic effector functions in NK γδ T cells. However, as a mechanism immune escape, ligands (NKG2DLs) can be released from cell surface. In this study, we investigated NKG2DL system different human glioblastoma (GBM) lines, most lethal brain adults. Flow cytometric analysis ELISA revealed that despite...
Despite aggressive treatment regimens based on surgery and radiochemotherapy, the prognosis of patients with grade IV glioblastoma multiforme (GBM) remains extremely poor, calling for alternative options such as immunotherapy. Immunological mechanisms including Natural Killer Group 2 member D (NKG2D) receptor-ligand system play an important role in tumor immune surveillance targeting NKG2D might be beneficial. However, before considering any kind immunotherapy, a precise characterization is...
Incoming viruses challenge the cell with diverse foreign molecules, which need to be sensed quickly initiate immune responses and remove viral components. In this study, we investigate cellular requirements for sensing degradation of incoming DNA capsids during herpes simplex virus type 1 (HSV-1) infections. Using click chemistry labeling genome, found that HSV-1 was released from a subset into cytosol early in infection. By next-generation sequencing cyclic GMP-AMP (cGAMP) synthase...
Innate immune sentinel cells must initiate and orchestrate appropriate responses for myriad pathogens. These stimulus-specific gene expression are mediated by combinatorial temporal coding within a handful of response signaling pathways. We outline the scope our current understanding indicate pressing outstanding questions.
Abstract Tumor necrosis factor (TNF) is a key inflammatory cytokine that warns recipient cells of nearby infection or tissue damage. Acute exposure to TNF activates characteristic oscillatory dynamics the transcription NFκB and induces gene expression program; these are distinct from responses directly exposed pathogen‐associated molecular patterns (PAMPs). Here, we report tonic critical for safeguarding TNF's specific functions. In absence conditioning, acute causes (i) signaling less more...
Significance Progress in understanding and treating inflammatory diseases relies on accurate characterization of the molecular pathology. From application nanoparticle tracking analysis, we expose a fundamental insight oligomeric protein structure as polyvalent stimulants autoreactive B cells. Quantification hydrodynamic radii implicates these large proteins part vascular damage, which is an important source morbidity death consequence autoimmunity. Dysregulated alterations concentration...
To address ongoing academic achievement gap, there is a need for more school-university partnerships promoting early access to STEM education. During summer 2020, members of our institute initiated QBio-EDGE (Quantitative Biology-Empowering Diversity and Growth in Education), an outreach program high schools Los Angeles. In the hope contributing increasing diversity academia, aims make education accessible students from historically excluded communities by exposing them scientific research...
Abstract Background The activating Natural killer group 2 member D (NKG2D) receptor is typically expressed on NK cells, CD8 T lymphocytes, γδ cells and small subsets of CD4 lymphocytes. During the course an extensive flow cytometry phenotyping immune in peripheral blood patients with glioblastoma multiforme (GBM) we noticed unexpected expression NKG2D granulocytes using phycoerythrin (PE)‐conjugated clone 149810 antibody. Methods Peripheral samples from 35 GBM 22 age‐matched healthy control...
Abstract Many transcription factors (TFs) translocate to the nucleus with varied dynamic patterns in response different inputs. A notable example of such behavior is RelA, a subunit NF-κB, which translocates either pulsed or sustained dynamics, depending on stimulus. Our understanding how these dynamics are interpreted by downstream genes has remained incomplete, partly because ubiquitously used environmental inputs activate other transcriptional regulators addition RelA. Here, we use an...
Abstract Acute and chronic inflammatory pathologies involve misregulation of macrophage functions. Physiologically, macrophages are immune sentinels that initiate responses via the transcription factor NFκB. The temporal pattern NFκB activity determines which genes expressed, suggesting a signaling code specifies stimulus-appropriate response. To identify codewords, we developed tools to enable high-throughput analysis live, primary responding host- pathogen-derived stimuli. An...
Inflammatory pathologies often involve dysregulated macrophage functions. In health, macrophages are immune sentinels that initiate inflammatory responses via the transcription factor NFkB. The temporal pattern of NFkB activity determines which genes expressed, but how such signaling code works in primary cells remains unexplored. For this study, we developed tools to enable high-throughput analysis live, responding host- and pathogen-derived stimuli. An information-theoretic workflow...
The education system in the US displays troubling disparities academic performance of different racial and income groups, known as achievement gap. During summer 2020, our research group committed to promoting ethnic diversity academia reduce gap Los Angeles. Since educators with a growth mindset employing active learning their classrooms can elevate students from underrepresented minority groups STEM fields, we initiated QBio-EDGE (Quantitative Biology - Empowering Diversity Growth...