A5008275837- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Trigeminal Neuralgia and Treatments
- Olfactory and Sensory Function Studies
- Ion Channels and Receptors
- Migraine and Headache Studies
- Botulinum Toxin and Related Neurological Disorders
- Cancer, Stress, Anesthesia, and Immune Response
- Neuroendocrine regulation and behavior
- Nitric Oxide and Endothelin Effects
- Stress Responses and Cortisol
- Anesthesia and Pain Management
- Nerve injury and regeneration
- Sexual function and dysfunction studies
- Dermatology and Skin Diseases
- Ion channel regulation and function
- Musculoskeletal pain and rehabilitation
- Pharmacological Effects of Natural Compounds
- Neuroscience of respiration and sleep
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Medicinal Plant Extracts Effects
- Essential Oils and Antimicrobial Activity
- Receptor Mechanisms and Signaling
- Plant-based Medicinal Research
- Vestibular and auditory disorders
Universidade Federal do Paraná
2015-2024
Universidade Federal de Santa Catarina
2006-2010
University of Arizona
2009
Abstract Objective Identification of the neural mechanisms underlying medication overuse headache resulting from triptans. Methods Triptans were administered systemically to rats by repeated intermittent injections or continuous infusion over 6 days. Periorbital and hind paw sensory thresholds measured detect cutaneous allodynia. Immunofluorescent histochemistry was employed changes in peptidic neurotransmitter expression identified dural afferents. Enzyme‐linked immunoabsorbent assay used...
Abstract Objective To develop and validate a model of cutaneous allodynia triggered by dural inflammation for pain associated with headaches. explore neural mechanisms underlying cephalic extracephalic allodynia. Methods Inflammatory mediators (IM) were applied to the dura unanesthetized rats via previously implanted cannulas, sensory thresholds face hind‐paws characterized. Results IM elicited robust facial hind‐paw allodynia, which peaked within 3 hours. These effects reminiscent seen in...
Migraine is a common neurological disorder often treated with triptans.Triptan overuse can lead to increased frequency of headache in some patients, phenomenon termed medication headache.Previous preclinical studies have demonstrated that repeated or sustained triptan administration for several days elicit persistent neural adaptations trigeminal ganglion cells innervating the dura, prominently characterized by labelling neuronal profiles calcitonin gene related peptide.Additionally,...
Objective The objective of this study was the determination role calcitonin gene-related peptide (CGRP) in induction medication overuse headache (MOH)-related migraine an injury-free preclinical model. Methods Rats were primed by a 7-day period exposure to acute therapies including sumatriptan and morphine. After additional 14-day drug-free period, rats exposed putative triggers bright light stress (BLS) or nitric oxide (NO) donor presence absence TEV48125, fully humanized CGRP antibody....
This study characterises some of the mechanisms and mediators involved in orofacial nociception triggered by injection formalin into upper lip rat, assessing influence various treatments on behavioural nociceptive responses (duration facial rubbing) elicited either a low subthreshold (i.e. non‐nociceptive; 0.63%) or higher concentration algogen (2.5%). The kininase II inhibitor captopril (5 mg kg −1 , s.c.) prostaglandin(PG) E 2 (100 ng ) potentiated both phases response to 0.63% formalin,...
The susceptibility of changes in responsiveness to noxious cold stimulation rats submitted chronic constriction the infraorbital nerve (CION) or carrageenan drug inhibition was compared. Nocifensive responses were measured as total time engaged bilateral facial grooming with both forepaws over first 2 min following tetrafluoroethane spray application snout. Carrageenan (50 microg, s.c. into upper lip) caused short-lived ipsilateral hyperalgesia (peak at 3 h: vehicle 8.4+/-1.3, 21.2+/-3.0 s)...
Trigeminal neuropathic pain has been modeled in rodents through the constriction of infraorbital nerve (CCI-ION). Sensory alterations, including spontaneous pain, and thermal mechanical hyperalgesia are well characterized, but there is a notable lack evidence about affective component this model. Evaluation emotional rats proposed as way to optimize potential translational value non-clinical studies. In rats, 22 50 kHz ultrasonic vocalizations (USVs) considered well-established measures...
Abstract Acute pain that persists for a few days is associated with reduction in patients' quality of life. Orofacial persistent promotes psychological disorders such as anxiety, impairs daily essential activities eating, and results decreased social interaction. Here, we investigated whether rats subjected to orofacial formalin injection or intraoral incision surgery display facial heat hyperalgesia, ongoing pain, anxiety-like behavior, changes ultrasonic vocalization. caused hyperalgesia 3...
Pain is a common symptom associated with disorders involving the orofacial structures. Most acute painful conditions are easily recognized, but pharmacological treatment may be limited by adverse events of current available drugs and/or patients' characteristics. In addition, chronic pain represent clinical challenges both, in terms diagnostic and treatment. There growing evidence that specialized pro-resolution lipid mediators (SPMs) present potent analgesic effects, addition to their well...
The trigeminal nerve is comprised of three main divisions, ophthalmic, maxillary and mandibular, each providing somatosensory innervation to distinct regions the head, face oral cavity. Recently, a role for endothelins in nociceptive signaling system has been proposed. present study aimed gain better insight into participation endothelin transmission. Herein ET-1 ET-3 mRNA was detected rats' ganglion (TG). Fluorescent labeling TG neurons revealed that ET(A) ET(B) receptors are distributed...
Objective To evaluate whether intraganglionic calcitonin gene-related peptide induced differential migraine-like responses in male and female rats. Methods Calcitonin was injected the trigeminal ganglion of rats followed by assessment periorbital mechanical allodynia with von Frey hairs. The influence systemic treatment sumatriptan or minocycline propentofylline determined on peptide-induced One additional group exposed to an aversive light 24 h after priming, evaluation threshold, another...
Increasing evidence indicates that endothelin-1 (ET-1) activates nociceptive neurons and sensitizes them to different noxious stimuli, but involvement of TRPV1-dependent mechanisms in mediation such effects is not yet fully understood. Here we report intraplantar (i.pl.) injection ET-1 (10 pmol) into the hind paw rats induced overt behavior over first hour, followed by a slowly developing thermal hyperalgesia, lasting from 3 8 h after injection. Both were also similar injections capsaicin...