A5014651573- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Genetics, Bioinformatics, and Biomedical Research
- Plant Pathogens and Resistance
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and Environmental Crop Studies
- Genomic variations and chromosomal abnormalities
- Genetics and Plant Breeding
- Prenatal Screening and Diagnostics
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Bioenergy crop production and management
- Hip and Femur Fractures
- Plant Genetic and Mutation Studies
- Microbial Community Ecology and Physiology
- Plant and fungal interactions
- Soybean genetics and cultivation
- Animal Genetics and Reproduction
- Photosynthetic Processes and Mechanisms
- Environmental DNA in Biodiversity Studies
- Research Data Management Practices
National Research Council Canada
2018-2024
Saskatchewan Research Council (Canada)
2019-2023
University of Oxford
2023
John Wiley & Sons (United Kingdom)
2023
Hudson Institute
2023
John Wiley & Sons (United States)
2023
Global Institute for Water Security
2018
University of Saskatchewan
2018
Diagnostic Services Manitoba
2012
Health Sciences Centre
2001-2009
Abstract Rye ( Secale cereale L.) is an exceptionally climate-resilient cereal crop, used extensively to produce improved wheat varieties via introgressive hybridization and possessing the entire repertoire of genes necessary enable hybrid breeding. allogamous only recently domesticated, thus giving cultivated ryes access a diverse exploitable wild gene pool. To further enhance agronomic potential rye, we produced chromosome-scale annotated assembly 7.9-gigabase rye genome validated its...
Abstract Legumes are an excellent source of proteins and health‐promoting phytochemicals. Recognizing their importance in human nutrition sustainable agricultural production, significant efforts currently being made to accelerate genetic gain related yield, stress resilience, nutritional quality. Recent increases genomic resources for multiple legume crops have laid a solid foundation application transformative breeding technologies such as selection genome editing crop improvement. In this...
Numerous scaffold-level sequences for wheat are now being released and, in this context, we report on a strategy improving the overall assembly to level comparable that of human genome. Using chromosome 7A as model, sequence-finished megabase-scale sections were established by combining new independent using bacterial artificial (BAC)-based physical map, BAC pool paired-end sequencing, chromosome-arm-specific mate-pair sequencing and Bionano optical mapping with International Wheat Genome...
Summary Awns, bristle‐like structures extending from grass lemmas, provide protection against predators, contribute to photosynthesis and aid in grain dispersal. In wheat, selection of awns with minimal extension, termed awnletted, has occurred during domestication by way loci that dominantly inhibit awn development, such as Tipped1 (B1) , Tipped2 (B2) Hooded (Hd) . Here we identify characterize the B1 gene. was identified using bulked segregant RNA ‐sequencing an F 2 durum wheat population...
Abstract Understanding the genomic relationship between wild and cultivated genomes would facilitate access to untapped variability found in crop relatives. We developed genome assemblies of a lentil ( Lens culinaris ) as well relative L. ervoides ). Comparative analyses revealed large-scale structural rearrangements additional repetitive DNA genome, resulting regions reduced recombination, segregation distortion permanent heterozygosity offspring cross two species. These novel findings...
Field-grown crops rarely experience growth conditions in which yield can be maximized. Environmental stresses occur combination, with advancements crop tolerance further complicated by its polygenic nature. Strategic targeting of causal genes is required to meet future production needs. Here, we employed a systems biology approach wheat (Triticum aestivum L.) investigate physio-metabolic adjustments and transcriptome reprogramming involved acclimations heat, drought, salinity all...
Breeding for Fusarium head blight (FHB) resistance in durum wheat is complicated by the quantitative trait expression and narrow genetic diversity of available resources. High-density mapping FHB loci (QTL), evaluation their co-localization with plant height maturity QTL interaction among identified are objectives this study. Two doubled haploid (DH) populations, one developed from crosses between Triticum turgidum ssp. lines DT707 DT696 other T. cv. Strongfield carthlicum Blackbird were...
Fusarium head blight (FHB) resistance in the durum wheat breeding gene pool is rarely reported. Triticum turgidum ssp. carthlicum line Blackbird a tetraploid relative of that offers partial FHB resistance. Resistance QTL were identified for cv. Strongfield × population on chromosomes 1A, 2A, 2B, 3A, 6A, 6B and 7B previous study. The objective this study was to identify defense mechanisms underlying report candidate regulator genes single nucleotide polymorphism (SNP) markers within these...
Summary The economically valuable Brassica species include the six related members of U’s Triangle. Despite agronomic and economic importance these Brassicas , impacts evolution relatively recent domestication events on genetic landscape seed development have not been comprehensively examined in species. Here we present a 3D transcriptome atlas for Triangle, producing unique resource that captures gene expression data major subcompartments seed, from unfertilized ovule to mature embryo coat....
The tall wheatgrass species Thinopyrum elongatum carries a strong fusarium head blight (FHB) resistance locus located on the long arm of chromosome 7 (7EL) as well to leaf and stem rusts, all diseases with significant impact wheat production. Towards understanding contribution Th. 7EL improvement disease in wheat, genomic sequence fragment present Chinese Spring (CS) telosomic addition line CS-7EL was determined rachis transcriptome during FHB infection compared between CS CS-7EL.We...
Lentil (Lens culinaris Medik.) is a nutritious legume with seeds rich in protein, minerals and an array of diverse specialized metabolites. The formation seed requires regulation tight coordination developmental programs to form the embryo, endosperm coat compartments, which determines structure composition mature thus its end-use quality. Understanding molecular cellular events metabolic processes development essential for improving lentil yield nutritional value. However, such information...
Global warming has been documented to threaten wild plants with strong selection pressures, but how plant populations respond genetically the threats remains poorly understood. We characterized genetic responses of 10 emmer wheat ( Triticum dicoccoides Koern.; WEW) in Israel, sampling them 1980 and again 2008, through an exome capture analysis. It was found that these WEW were under elevated selection, displayed reduced diversity temporal divergence, carried increased mutational burdens...
Four QTL for ergot resistance (causal pathogen Claviceps purpurea) have been identified in the durum wheat cultivar Greenshank. purpurea is a of grasses that infects flowers, replacing seed with an sclerotium. Ergot presents significant problem to rye, barley and wheat, particular hybrid production systems. In addition, there evidence highly toxic alkaloids accumulate within sclerotia can cross-contaminate otherwise healthy grain. Host C. rare, few loci having identified. this study, four...
High-throughput genotyping enables the large-scale analysis of genetic diversity in population genomics and genome-wide association studies that combine genotypic phenotypic characterization large collections accessions. Sequencing-based approaches for are progressively replacing traditional methods because lower ascertainment bias. However, based on sequencing becomes expensive species with genomes a high proportion repetitive DNA. Here we describe use CRISPR-Cas9 technology to deplete...
Abstract We present a chromosome-scale annotated assembly of the rye ( Secale cereale L. inbred line ‘Lo7’) genome, which we use to explore Triticeae genomic evolution, and rye’s superior disease stress tolerance. The genome shares chromosome-level organization with other cereals, but exhibits unique retrotransposon dynamics structural features. Crop improvement in rye, as well wheat triticale, will profit from investigations gene families implicated pathogen resistance, low temperature...
The durum wheat line DT696 is a source of moderate Fusarium head blight (FHB) resistance. Previous analysis using bi-parental population identified two FHB resistance quantitative trait loci (QTL) on chromosome 5A: 5A1 was co-located with plant height QTL, and 5A2 major maturity QTL. A Genome-Wide Association Study (GWAS) derivative lines from 72 crosses based multi-environment resistance, height, phenotypic data conducted to improve the mapping resolution further elucidate genetic...
Abstract Background Fusarium head blight (FHB) infection results in damaged kernels (FDK) and deoxynivalenol (DON) contamination that are downgrading factors at the Canadian elevators. Durum wheat ( Triticum turgidum L. var. durum Desf.) is particularly susceptible to FHB most of adapted cultivars moderately this disease. However, line DT696 less than commercially grown cultivars. Little known about genetic variation for ability resist FDK DON accumulation. This study was undertaken map loci...
Reproductive success hinges on precisely coordinated meiosis, yet our understanding of how structural rearrangements chromatin and phase transitions during meiosis are transcriptionally regulated is limited. In crop plants, detailed analysis the meiotic transcriptome could identify regulatory genes epigenetic regulators that can be targeted to increase recombination rates broaden genetic variation, as well provide a resource for comparison among eukaryotes different taxa answer outstanding...
The tall wheatgrass species Thinopyrum elongatum carries on the long arm of chromosome 7E, a locus that contributes strongly to resistance fusarium head blight (FHB), devastating fungal disease affecting wheat crops in all temperate areas world. Introgression Th. 7E chromatin into 7D was induced by ph1b mutant CS. Recombinants between and 7D, mutation, were monitored combination molecular markers phenotyping for FHB resistance. Progeny up five subsequent generations derived from two...
We report an 8-year-old girl with coarse facial features, macrocrania and developmental delay. Cranial anomalies in the form of hydrocephalus Dandy-Walker (DW) variant malformation were detected on neuro-imaging. Karyotyping revealed a de novo interstitial deletion bands 3q25.1 to 3q25.33. Deletion 3q24-q26 region appears be associated somewhat similar constellation findings craniofacial dysmorphism (broad depressed nasal bridge low set posteriorly rotated ears), mental retardation,...
Cytogenetic studies of a mildly dysmorphic 10-year-old male with mild developmental delay and learning difficulties revealed mosaicism for supernumerary ring chromosome in approximately half the cells. The karyotype this patient was established as 47,XY,+r[15]/46,XY[15].ish r(1)(D1Z7+,wcp1−). Although presence euchromatic material shown by C banding, lack hybridization whole paint 1 (wcp1) probe suggests that few unique sequences are contained these likely explain child's features delay. A...