A5028750912- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Toxin Mechanisms and Immunotoxins
- Insect-Plant Interactions and Control
- Biofuel production and bioconversion
- Plant Pathogenic Bacteria Studies
- Plant nutrient uptake and metabolism
- Bioenergy crop production and management
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Mosquito-borne diseases and control
National Agricultural Library
2020-2022
Agricultural Research Service
2021
Beltsville Agricultural Research Center
2021
United States Department of Agriculture
2021
Plant (United States)
2019
University of California, Davis
2017-2019
Clemson University
2011
Puccinia graminis f. sp. tritici (Pgt) causes wheat stem rust, a devastating fungal disease. The Sr35 resistance gene confers immunity against this pathogen's most virulent races, including Ug99. We used comparative whole-genome sequencing of chemically mutagenized and natural Pgt isolates to identify named AvrSr35 that is required for avirulence. encodes secreted protein capable interacting with triggering the immune response. show origin on associated nonfunctionalization by insertion...
Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a devastating foliar disease. The Ug99 race group has combined virulence to most rust (Sr) resistance genes deployed in wheat and threat global production. Here we identified coiled-coil, nucleotide-binding leucine-rich repeat protein (NLR) completely linked the gene Sr21 from Triticum monococcum. Loss-of-function mutations transgenic complementation confirmed that this Sr21. transcripts were significantly higher at high...
Nucleotide-binding leucine-rich repeat receptors (NLRs) are the most abundant type of immune in plants and can trigger a rapid cell-death (hypersensitive) response upon sensing pathogens. We previously cloned wheat NLR Sr35, which encodes coiled-coil (CC) that confers resistance to virulent stem rust race Ug99. Here, we investigated Sr35 signaling after Agrobacterium-mediated transient expression Nicotiana benthamiana. Expression N. benthamiana leaves triggered mild response, is enhanced...
Abstract High throughput sequencing (HTS) can supplement and may replace diagnostic tests for plant pathogens. However, the methodology processing of HTS data must first be optimized standardized to ensure sensitivity repeatability results. Importation sugarcane into United States is highly regulated, plants are subjected strict quarantine measures testing, especially presence certain viruses regulatory concern. Here, we tested whether could reliably detect four RNA three DNA over seasons...
Miscanthus sinensis is a grass used for sugarcane breeding and bioenergy production. Using high throughput sequencing technologies, we identified new viral genome in infected M. leaf tissue displaying yellow fleck symptoms. This virus most related to members of the genus Polerovirus family Luteoviridae. The canonical ORFs were computationally identified, P3 coat protein was expressed, virus-like particles purified found conform icosahedral shapes, characteristic We propose name this virus.
Abstract The complete genome sequence of a previously uncharacterized sugarcane‐infecting virus the genus Ampelovirus was obtained by high‐throughput sequencing (HTS) and rapid amplification cDNA ends. This consists 13,114 nucleotides (nt) harbours seven open reading frames. Phylogenetic pairwise identity analyses with or near‐complete sequences 16 isolates revealed that they all belong to new species, family Closteroviridae . These were distributed in three phylogenetic groups potential...
Maize stripe virus is a pathogen of corn and sorghum in subtropical tropical regions worldwide. We used high-throughput sequencing to obtain the complete nucleotide sequence for reference genome maize genomes ten additional isolates collected from United States or Papua New Guinea. Genetically, most closely related rice virus. completed characterized RNA1 virus, which revealed large open reading frame encoding putative protein with ovarian tumor-like cysteine protease, endonuclease,...
Abscisic acid (ABA) and indole-3-acetic (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation embryo.The goal assay was to compare endogenous levels ABA IAA between embryos (SE) zygotic (ZE) Nothofagus alpina (Poepp.& Endl.)Oerst.In this study, a embryo maturation involving addition varying concentrations exogenous performed on cotyledonary-stage N. alpina.Furthermore, were quantified immature ZE, mature embryonic axis alpina.The...