A5052670756- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Machine Learning in Bioinformatics
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Pneumonia and Respiratory Infections
- Protist diversity and phylogeny
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Bacterial biofilms and quorum sensing
- Tuberculosis Research and Epidemiology
- RNA Research and Splicing
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolic Engineering and Bioproduction
- Plant Pathogenic Bacteria Studies
- Machine Learning and Algorithms
- Escherichia coli research studies
- Mycobacterium research and diagnosis
- Probiotics and Fermented Foods
- Advanced biosensing and bioanalysis techniques
- Bioinformatics and Genomic Networks
- AI-based Problem Solving and Planning
- Galectins and Cancer Biology
Centre National de la Recherche Scientifique
2012-2024
Université de Toulouse
2010-2024
Université Toulouse III - Paul Sabatier
2009-2023
Laboratoire de Microbiologie et Génétique Moléculaires
2011-2023
Laboratoire de Biologie Moléculaire des Eucaryotes
2010
Laboratoire des Interactions Plantes Micro-Organismes
2007
Laboratoire de Génétique Cellulaire
2007
Centre d’Immunologie de Marseille-Luminy
2005
Institut de Biologie Structurale
1999-2003
Laboratoire de Chimie Bactérienne
1999-2003
Abstract Background The availability of hundreds bacterial genomes allowed a comparative genomic study the Type VI Secretion System (T6SS), recently discovered as being involved in pathogenesis. By combining and phylogenetic approaches using more than 500 prokaryotic genomes, we characterized global T6SS genetic structure terms conservation, evolution organization. Results This genome wide analysis identification set 13 proteins constituting protein core conserved accessory proteins. 176...
Our knowledge of the functions metazoan ribosomal proteins in ribosome synthesis remains fragmentary. Using siRNAs, we show that knockdown 31 32 human 40S subunit (ribosomal protein small [RPS]) strongly affects pre-ribosomal RNA (rRNA) processing, which often correlates with nucleolar chromatin disorganization. 16 RPSs are strictly required for initiating processing sequences flanking 18S rRNA pre-rRNA except at metazoan-specific early cleavage site. The remaining necessary progression...
Significance This article concerns the control of competence for bacterial genetic transformation. Competence is transient in human pathogen Streptococcus pneumoniae , involving specific expression ∼100 genes that are turned ON suddenly and OFF almost as abruptly. Although mechanism rendering all cells a culture simultaneously competent well understood, how stops has remained unknown. Here, we unravel shut-off, describing discovery key recombination protein, DprA, exerts negative on through...
Summary The opportunistic pathogen Pseudomonas aeruginosa has redundant molecular systems that contribute to its pathogenicity. Those assembling fimbrial structures promote complex organized community lifestyle. We characterized a new 5.8 kb genetic locus, cupE , includes the conserved usher‐ and chaperone‐encoding genes. This widely in different bacterial species, contains four additional genes encoding non‐archetypal subunits. first evidenced gene cluster was specifically expressed biofilm...
To investigate the resistance mechanisms of β-lactam-resistant Pseudomonas aeruginosa isolated from cystic fibrosis (CF) patients in France. Two-hundred-and-four P. CF isolates were collected 10 French university hospitals 2007. Their susceptibility to 14 antibiotics and their β-lactams investigated. β-lactamase contents characterized by isoelectric focusing, PCR enzymatic assays. Expression levels efflux pumps intrinsic AmpC quantified reverse transcription real-time quantitative PCR....
REPs are highly repeated intergenic palindromic sequences often clustered into structures called BIMEs including two individual separated by short linker of variable length. They play a variety key roles in the cell. also resemble sub-terminal hairpins atypical IS 200/605 family insertion which encode Y1 transposases (TnpA IS200/IS605 ). These belong to HUH endonuclease family, carry single catalytic tyrosine (Y) and promote strand transposition. Recently, new clade REP ) was found...
Protein coding regions of a genome fragment can be mathematically predicted by studying variations in the statistical properties or searching signals characteristic junctions between and non-coding regions. We propose here new method using correspondence analysis. This does not use any reference codon set but takes into account usage homogeneity along studied fragment. Comparison with previously published methods especially 'codon method' Staden has been made, two examples are presented...
Ramlibacter tataouinensis TTB310T (strain TTB310), a betaproteobacterium isolated from semi-arid region of South Tunisia (Tataouine), is characterized by the presence both spherical and rod-shaped cells in pure culture. Cell division strain TTB310 occurs binary fission "cyst-like" ("cyst-cyst" division). The formed at periphery colony (consisting mainly cysts) are highly motile colonize new environment, where they form reversion to cyst-like cells. This unique cell cycle TTB310, with...
Streptococcus pneumoniae is a naturally transformable human pathogen. Genome and phylogenetic analyses uncovered two Spx-like global transcriptional regulators, SpxA1 SpxA2, encoded by S. pneumoniae. spxA1 spxA2 are not essential, but their simultaneous inactivation lethal. represses transcription of the early competence operon comCDE thereby negatively regulates initiation X-state (competence). The molecular basis this repression could be similar to that SpxA Bacillus subtilis, involving...
Mycobacterium tuberculosis, the etiological agent of is a Gram-positive bacterium with unique cell envelope composed an essential outer membrane. Mycolic acids, which are very-long-chain (up to C100) fatty major components this mycomembrane. The enzymatic pathways involved in biosynthesis and transport mycolates fairly well documented targets antituberculous drugs. In contrast, only fragmented information available on expression regulation genes. study, we report that hadA, hadB, hadC genes,...
ABSTRACT How split genomes arise and evolve in bacteria is poorly understood. Since each replicon of such encodes a specific partition (Par) system, the evolution Par systems could shed light on their evolution. The cystic fibrosis pathogen Burkholderia cenocepacia has three chromosomes (c1, c2, c3) one plasmid (pBC), whose compatibility depends strictly interactions centromere sequences ( parS ) with cognate binding proteins (ParB). However, B. c3, pBC share many features, suggesting that...
In the human pathogen Streptococcus pneumoniae, gene regulatory circuit leading to transient state of competence for natural transformation is based on production an auto-inducer that activates a positive feedback loop. About 100 genes are activated in two successive waves linked by central alternative sigma factor ComX. This mechanism appears be fundamental biological fitness S. pneumoniae. We have developed knowledge-based model cycle describes average cell behavior. It reveals expression...
Abstract A network of RNA helicases, endoribonucleases and exoribonucleases regulates the quantity quality cellular RNAs. To date, mechanistic studies focussed on bacterial eukaryal systems due to challenge identifying main drivers decay processing in Archaea. Here, our data support that aRNase J, a 5′-3′ exoribonuclease β-CASP family conserved Euryarchaeota, engages specifically with Ski2-like helicase exosome potentially exert control over surveillance, at vicinity ribosome. Proteomic...
The ATP-binding cassette (ABC) transporters are one of the major classes active transporters. They widespread in archaea, bacteria, and eukaryota, indicating that they have arisen early evolution. involved many essential physiological processes, but majority import or export a wide variety compounds across cellular membranes. These systems share common architecture composed four (exporters) five (importers) domains. To identify reconstruct functional ABC encoded by archaeal bacterial...
Enterococcus faecalis is a commensal bacterium of the gastrointestinal tract but also major nosocomial pathogen. This uses regulators like BglG/SacY family transcriptional antiterminators to adapt its metabolism during host colonization. In this report, we investigated role antiterminator NagY in regulation nagY-nagE operon presence N-acetylglucosamine, with nagE encoding transporter carbohydrate, as well expression virulence factor HylA. We showed that last protein involved biofilm...