A5051045948- Nanoparticle-Based Drug Delivery
- Click Chemistry and Applications
- Characterization and Applications of Magnetic Nanoparticles
- Advanced biosensing and bioanalysis techniques
- Photoacoustic and Ultrasonic Imaging
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Chemical Synthesis and Analysis
- Ultrasound and Hyperthermia Applications
- Synthesis of β-Lactam Compounds
- Nanoplatforms for cancer theranostics
- Crystallography and molecular interactions
- Monoclonal and Polyclonal Antibodies Research
- Electrospun Nanofibers in Biomedical Applications
- Microfluidic and Bio-sensing Technologies
- Cyclopropane Reaction Mechanisms
- Asymmetric Synthesis and Catalysis
- Iron oxide chemistry and applications
- Cardiac Fibrosis and Remodeling
- Cancer Research and Treatments
- Graphene and Nanomaterials Applications
- Advanced NMR Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Gold and Silver Nanoparticles Synthesis and Applications
- Tissue Engineering and Regenerative Medicine
Instituto de Nanociencia y Materiales de Aragón
2017-2025
Universidad de Zaragoza
2014-2024
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2017-2024
Instituto de Salud Carlos III
2022
Centro de Investigación Biomédica en Red
2021
Instituto de Ciencia de Materiales de Sevilla
2019
Centro Médico Sanitas Zaragoza
2017
University of Twente
2010-2016
Fundacion Agencia Aragonesa para la Investigacion y el Desarrollo
2014-2015
University of the Basque Country
2003-2014
The protein corona formed on the surface of a nanoparticle in biological medium determines its behavior vivo. Herein, iron oxide nanoparticles containing same core and shell, but bearing two different coatings, either glucose or poly(ethylene glycol), were evaluated. nanoparticles' adsorption, vitro degradation, vivo biodistribution biotransformation over four months investigated. Although both types bound similar amounts proteins vitro, differences composition correlated to Interestingly,...
Accessing 1,2,3-triazole compounds through the copper-catalysed azide–alkyne "click" cycloaddition reaction is now a routine synthetic tool which has resulted in huge amount of novel molecules varied complexity bearing such heterocycles. 3-N-Alkyl-1,2,3-triazolium salts constitute an example "post-click" chemistry arising from pool. This Focus review outlines emerging fields application for triazolium cations, including their use as functional ionic liquids, precursors mesoionic carbenes, or...
Detection of tumor metastases in the lymphatic system is essential for accurate staging malignancies. Commercially available superparagmagnetic nanoparticles (SPIOs) accumulate normal lymph tissue after injection at a site, whereas less or no accumulation takes place metastatic nodes, thus enabling using MRI. We verify first time potential SPIOs, such as Endorem® novel photoacoustic (PA) contrast agent biological tissue. injected five Wistar rats subcutaneously with variable amounts and...
Magnetic hyperthermia is a cancer treatment based on the exposure of magnetic nanoparticles to an alternating field in order generate local heat. In this work, 3D cell culture models were prepared observe effect that different number internalized particles had mechanisms death triggered upon treatment. Macrophages selected by their high capacity uptake nanoparticles. Intracellular nanoparticle concentrations up 7.5 pg Fe/cell measured both elemental analysis and characterization techniques....
Magnetic hyperthermia (MH) was used to treat a murine model of pancreatic cancer. This type cancer is generally characterized by the presence dense stroma that acts as barrier for chemotherapeutic treatments. Several alternating magnetic field (AMF) conditions were evaluated using three-dimensional (3D) cell culture models loaded with nanoparticles (MNPs) determine which producing strong effect on viability. Once optimal AMF selected, in vivo experiments carried out similar frequency and...
As few as 23 enzyme molecules could be detected on the basis of dispersion Au nanoparticles in a model bioassay whose sensitivity was boosted by interplay between multivalent and monovalent supramolecular interactions: diferrocenyl ligand (red) caused assembly nanoparticle clusters; upon its oxidation, competing guest molecule (blue) favored formation interactions for more efficient disassembly. Nanoparticles are becoming extremely useful building blocks fabrication ultrasensitive optical1...
Magnetic hyperthermia is a promising therapy for the localized treatment of cancer based on exposure magnetic nanoparticles to an external alternating field. In order evaluate some mechanisms involved in cellular damage caused by this treatment, two different 3D cell culture models were prepared using collagen, which most abundant protein extracellular matrix. The same amount was added cells either before or after their incorporation into structure. Therefore, one model, particles located...
This study describes that the current rectification ratio, R ≡ |J|(−2.0 V)/|J|(+2.0 V) for supramolecular tunneling junctions with a top-electrode of eutectic gallium indium (EGaIn) contains conductive thin (0.7 nm) supporting outer oxide layer (Ga2O3), increases by up to four orders magnitude under an applied bias >+1.0 V +2.5 V; these did not change their electrical characteristics when biased in voltage range ±1.0 V. The increase is caused presence water and ions assemblies which react...
Unsymmetrically 1,1′-disubstituted 4,4′-bis-1H-1,2,3-triazoles 4 have been prepared from 4-ethynyl-1,2,3-triazoles 5 and azides. Following a "double-click" strategy, two complementary approaches were implemented for the preparation of key 4-ethynyltriazole intermediates 5: (a) stepwise Swern oxidation/Ohira−Bestman alkynylation readily available 4-hydroxymethyl-1,2,3-triazoles 8 (b) cycloaddition TMS-1,4-butadiyne 9. The method is highlighted by its compatibility with orthogonally protected...
This work aims at studying how the transformations that magnetic nanoparticles suffer in vivo affect their heating properties frame of hyperthermia treatments. Iron oxide (≈13 nm) with two different coatings [PMAO (polymaleic anhydride-alt-1-octadecene) and DMSA (dimercaptosuccinic acid)] have been subjected to an accelerated degradation a medium simulating lysosome conditions. The particles physicochemical (size, size distribution, properties) followed over process along 24 days. It was...
Ring opening of α-substituted-α-methoxycarbonyl-N-nosylaziridines provides a practical access to enantiopure α,α'-disubstituted β-lactam scaffolds, novel types ditopic reverse turn surrogates. The procedure is general, short, and high yielding starts from handy α-substituted serinates α-amino acid derivatives.
Silver nanoparticles have particular properties that contribute to their very promising applications, novel in various fields of science, such as the development biosensors, diagnosis and treatment cancer, controlled release drugs antimicrobial potential.The biological synthesis is great interest over other physical chemical methods because use toxic chemicals drastic reaction conditions are avoided.The extracellular biosynthesis using fungi could also make downstream processing much easier...
Lipids are a major source of energy for most tissues, and lipid uptake storage is therefore crucial homeostasis. So far, quantification in vivo has primarily relied on radioactive isotope labeling, exposing human subjects or experimental animals to ionizing radiation. Here, we describe the chylomicrons, primary carriers dietary lipids, metabolically active tissues using magnetic particle imaging (MPI) spectroscopy (MPS). We show that loading artificial chylomicrons (ACM) with iron oxide...
Novel enantiopure (i)-(β-lactam)-(Gly)-(i+3) peptide models, defined by the presence of a central α-alkyl-α-amino-β-lactam ring placed as (i+1) residue, have been synthesized in totally stereocontrolled way α-alkylation suitable N-[bis(trimethylsilyl)methyl]-β-lactams. The structural properties these β-lactam pseudopeptides studied X-ray crystallography, Molecular Dynamics simulation, and NOESY-restrained NMR simulated annealing techniques, showing strong tendency to form stable type II or...
Mesoionic 4,4'-bis(1,2,3-triazole-5,5'-diylidene) Rh(I) complexes having a C2 chiral 4,4'-axis were accessed from 3-alkyltriazolium salts in virtually complete de. Their structure and configurational integrity assessed by NMR spectroscopy, X-ray crystallography, HPLC. Computational analysis of the MICs involved reaction suggested formation highly stable unprecedented cation-carbene intermediate species, which could be evidenced experimentally cyclic voltammetry analysis.
Conventional methods to determine the kinetic parameters for a certain reaction require multiple, separate isothermal experiments, resulting in time- and material-consuming processes. Here, an approach information within single nonisothermal on-flow experiment is presented, consuming less than 10 μmol of reagents having total measuring time typically min. This makes use microfluidic NMR chip hyphenated continuous-flow microreactor based on capabilities analyze subnanomole quantities material...