A5044898825- Gut microbiota and health
- Gastrointestinal motility and disorders
- Helicobacter pylori-related gastroenterology studies
- Immune Response and Inflammation
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Diet and metabolism studies
- Digestive system and related health
- Glycosylation and Glycoproteins Research
- Diet, Metabolism, and Disease
- Immune cells in cancer
- Clostridium difficile and Clostridium perfringens research
- Tryptophan and brain disorders
- Pancreatitis Pathology and Treatment
- Inflammatory Bowel Disease
- Sulfur Compounds in Biology
- Protein Hydrolysis and Bioactive Peptides
- Magnesium in Health and Disease
- Vagus Nerve Stimulation Research
- Listeria monocytogenes in Food Safety
- Eicosanoids and Hypertension Pharmacology
- Inflammasome and immune disorders
- Neuropeptides and Animal Physiology
- Cytokine Signaling Pathways and Interactions
- Nicotinic Acetylcholine Receptors Study
Centro de Investigación y Tecnología Agroalimentaria de Aragón
2016-2024
Universidad de Zaragoza
2013-2024
Instituto de Investigación Sanitaria Aragón
2016-2024
University of Gothenburg
2019-2024
Institute for Biomedicine
2024
University of Zurich
2019
If not properly regulated, the inflammatory immune response can promote carcinogenesis, as evident in colorectal cancer (CRC). Aiming to gain mechanistic insight into link between inflammation and CRC, we perform transcriptomics analysis of human identifying a strong correlation expression serine protease granzyme A (GzmA) inflammation. In dextran sodium sulfate azoxymethane (DSS/AOM) mouse model, deficiency pharmacological inhibition extracellular GzmA both attenuate gut prevent CRC...
Serotonin (5-HT) is a key neurotransmitter synthesized both in the gut and central nervous system. It exerts its signaling through specific receptors (5-HTR), which regulate numerous behaviors functions such as mood, cognitive function, platelet aggregation, gastrointestinal motility, inflammation. activity determined mainly by extracellular availability of 5-HT, controlled serotonin transporter (SERT). Recent studies indicate that, activation innate immunity receptors, microbiota can...
The intestine is under constant exposure to chemicals, antigens, and microorganisms from the external environment. Apical aspects of transporting epithelial cells (enterocytes) form a brush-border membrane (BBM), shaped by packed microvilli coated with dense glycocalyx. We present evidence showing that glycocalyx forms an barrier prevents exogenous molecules live bacteria gaining access BBM. use multi-omics approach investigate function regulation mucins exposed on BBM during postnatal...
Intestinal inflammatory diseases are the result of multiple processes, including mucosal oxidative stress and perturbed homeostasis between commensal bacteria immunity. Toll-like receptors (TLRs) recognize molecular-associated microorganisms' patterns trigger innate immunity responses contributing to intestinal responses. However, TLRs effects on redox balance in mucosa remain unknown. Therefore, present study analyzes effect TLR2, TLR3, TLR4 both damage lipids proteins, activity antioxidant...
TLR2 is a microbiota recognition receptor that has been described to contribute intestinal homeostasis and ameliorate inflammatory injury. In this context, serotonin (5-HT) shown be an essential physiological neuromodulator also involved in diseases. Since the interaction between activation serotoninergic system remains non-investigated, our main aim was analyze effect of on transporter (SERT) activity expression intracellular pathways involved. Caco-2/TC7 cells were used SERT molecular by...
Oxidative stress is thought to play a key role in the development of intestinal damage inflammatory diseases. Several molecules are involved inflammation, either as pro- or anti-inflammatory factors; however, their effects on oxidative seem be controversial. This work analyzes contribution and balance epithelial cells, well cellular antioxidant enzyme activity. With this purpose, lipid protein oxidation, together with activity catalase, superoxide dismutase, glutathione peroxidase, were...
Inflammatory bowel diseases are consequence of an intestinal homeostasis breakdown in which innate immune dysregulation is implicated. Toll-like receptor (TLR)2 and TLR4 recognition receptors expressed the epithelium, first physical-physiological barrier for microorganisms, to inform host presence Gram-positive Gram-negative organisms. Interleukin (IL)-10 essential anti-inflammatory cytokine that contributes maintenance homeostasis.Our main aim was investigate IL-10 synthesis release,...
bacitracina disminuyen la permeabilidad intestinal
Serotonin (5-HT) is an essential gastrointestinal modulator whose effects regulate the intestinal physiology. 5-HT depend on extracellular bioavailability, which controlled by serotonin transporter (SERT) expressed in both apical and basolateral membranes of enterocytes. SERT a critical target for regulating levels consequently, modulating The deregulation innate immune receptors has been extensively studied inflammatory bowel diseases (IBD), where exacerbated defense response to commensal...
Abstract Crohn’s disease (CD) is the chronic inflammation of ileum and colon triggered by bacteria, but insights into molecular perturbations at bacteria-epithelium interface are limited. We report that membrane mucin MUC17 protects small intestinal enterocytes against commensal pathogenic bacteria. In non-inflamed CD ileum, reduced levels correlated with a compromised glycocalyx, allowing bacterial contact enterocytes. Muc17 deletion in mice rendered intestine prone to atypical infection...
Listeria monocytogenes is a Gram-positive bacterium that can cause serious infection. Intestinal microorganisms have been demonstrated to contribute intestinal physiology not only through immunological responses but also by modulating the serotonergic system. Serotonin (5-HT) neuromodulator synthesized in epithelium and regulates whole physiology. The serotonin transporter (SERT), located enterocytes, controls 5-HT availability therefore serotonin's effects. Infections caused L. are well...
Background/Aims: Serotonin (5-HT) is a chief modulator of intestinal activity. The effects 5-HT depend on its extracellular availability, which mainly controlled by serotonin transporter (SERT), expressed in enterocytes. On the other hand, innate immunity, mediated Toll-like receptors (TLRs) and nucleotide oligomerization domain (NOD)-like (NLRs), known to control microbiota maintain homeostasis. dysregulation serotonergic system immunity has been observed inflammatory bowel diseases (IBD),...
In the distal colon, mucus secreting goblet cells primarily confer protection from luminal microorganisms via generation of a sterile inner layer barrier structure. Bacteria-sensing sentinel provide secondary defensive mechanism that orchestrates secretion in response to microbes breach barrier. Previous reports have identified deficiencies adult germ-free mice, thus implicating fundamental role for microbiota programming generation. this study, we investigated natural neonatal development...
Abstract Background H 2 S is a neuromodulator that may inhibit intestinal motility. production in colon yielded by cystathionine β‐synthase (CBS) and γ‐lyase (CSE) enzymes sulfate‐reducing bacteria (SRB). Toll‐like receptors (TLRs) recognize microbiota. The aim of this work was to evaluate the influence TLR2 TLR4 on endogenous SRB‐mediated synthesis its consequences colonic motility mouse. Methods Muscle contractility studies were performed from WT, Tlr2 ‐/‐ , Tlr4 mice. mRNA levels TLR2,...
Irritable bowel syndrome (IBS) is a chronic functional disorder characterized by intestinal dysmotility. Changes in microbiota (dysbiosis) can lead to alterations neuro-muscular functions the gut. Toll-like receptors (TLRs) 2 and 4 recognize bacteria are involved motor response induced gastrointestinal (GI) neurotransmitters. Acetylcholine (ACh) well-known neurotransmitter regulation of GI motility. This study aimed evaluate role TLR2 TLR4 motor-response ACh mouse ileum, as well expression...
Dairy-derived bioactive components have health-promoting effects due to their large number of biological properties.Although the physiological significance several these substances is not yet fully understood, both proteins and peptides are now believed be health-enhancing components.Serotonin a key signalling neurotransmitter for gut-brain axis that controls wide range functions, highlighting its regulation neurobiological intestinal physiology.Here, we discuss roles pathways whereby...
Phosphate (Pi) homeostasis is controlled by the intestine and kidneys whose capacities to transport Pi are under endocrine control. Several studies point intestinal absorption as a therapeutic target modulate homeostasis. The small responsible for almost all in gut, process involving Na+-dependent independent mechanisms. Three cotransporters have been described gastrointestinal tract: NaPi-IIb (a SLC34 member) Pit-1 Pit-2 (SLC20 transporters). We recently analysed acute hormonal renal...
Abstract Intestinal serotonergic system is a key modulator of intestinal homeostasis; however, its regulation still unclear. Toll-like receptor 9 (TLR9), an innate immune receptor, detects different external agents in the intestine, preserving integrity. Since little known about TLR9 role our aim was to address potential between and system. Caco-2/TC7 cell line tract Tlr9 −/− mice were used this study. Serotonin uptake studies performed, molecular expression components analyzed by western...
Crohn's disease (CD) is the chronic inflammation of terminal ileum and colon triggered by a dysregulated immune response to bacteria, but insights into specific molecular perturbations at critical bacteria-epithelium interface are limited. Here we report that membrane mucin MUC17 protected small intestinal enterocytes against commensal pathogenic bacteria. In non-inflamed CD ileum, reduced levels compromised glycocalyx barrier allowed recurrent bacterial contact with enterocytes. Muc17...