A5054502784- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- HER2/EGFR in Cancer Research
- Metabolism and Genetic Disorders
- Advanced Breast Cancer Therapies
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Genetic and Kidney Cyst Diseases
- Genomics and Chromatin Dynamics
- Mathematical Biology Tumor Growth
- Cytokine Signaling Pathways and Interactions
- Diet and metabolism studies
- Reproductive Biology and Fertility
- Chromatin Remodeling and Cancer
- Gene Regulatory Network Analysis
- Renal and related cancers
- Immune Cell Function and Interaction
- Mathematical and Theoretical Epidemiology and Ecology Models
- Breast Cancer Treatment Studies
- Microbial Metabolic Engineering and Bioproduction
- Orthopedic Infections and Treatments
- Folate and B Vitamins Research
- Peroxisome Proliferator-Activated Receptors
- Total Knee Arthroplasty Outcomes
- Orthopaedic implants and arthroplasty
Hospital Del Mar
2013-2024
Municipal Institute for Medical Research
2000-2023
Barcelona Biomedical Research Park
2008-2023
Hospital del Mar Research Institute
2013-2022
Parc de Salut
2014
Hospital de l'Esperança
2011
Universitat Pompeu Fabra
2002-2005
The Netherlands Cancer Institute
1996-1999
Oncode Institute
1996-1998
Universitat de Barcelona
1990-1995
Fatty acids induce an increase in the transcription of mitochondrial 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase gene, which encodes enzyme that has been proposed as a control site ketogenesis. We studied whether peroxisome proliferator-activated receptor (PPAR) is involved mechanism this transcriptional induction. found cotransfection rat HMG-CoA promoter-chloramphenicol acetyltransferase reporter plasmid and PPAR expression presence proliferator clofibrate led to more than 30-fold...
Purpose: Trastuzumab-emtansine (T-DM1) is a standard treatment in advanced HER2-positive breast cancer. However, resistance inevitably occurs. We aimed to identify mechanisms of acquired T-DM1 resistance.Experimental Design: cancer cells (HCC1954, HCC1419, SKBR3, and BT474) were treated pulse-fashion with induce resistant phenotype. Cellular molecular effects parental versus compared. CDK1 kinase activity cyclin B1 expression assayed under various conditions. Genetic modifications up- or...
GEMC1 and MCIDAS are Geminin family proteins that transcriptionally activate E2F4/5-target genes during multiciliogenesis, including FoxJ1 Ccno. Male mice lacking Gemc1, Mcidas or Ccno were found to be infertile, but the origin of this defect has remained unclear. Here we show all three necessary for generation functional multiciliated cells in efferent ducts required spermatozoa enter epididymis. In mutant Ccno, observed a similar spectrum phenotypes, thinning seminiferous tubule epithelia,...
If not properly regulated, the inflammatory immune response can promote carcinogenesis, as evident in colorectal cancer (CRC). Aiming to gain mechanistic insight into link between inflammation and CRC, we perform transcriptomics analysis of human identifying a strong correlation expression serine protease granzyme A (GzmA) inflammation. In dextran sodium sulfate azoxymethane (DSS/AOM) mouse model, deficiency pharmacological inhibition extracellular GzmA both attenuate gut prevent CRC...
Bad is a distant relative of Bcl-2 and acts to promote cell death. Here, we show that expression levels are greatly increased in thymocytes during apoptosis. We generated bad transgenic mice study the action upregulated on T The cells from these highly sensitive apoptotic stimuli, including anti-CD95. numbers depleted processes development selection perturbed. proapoptotic function primary regulated by Akt kinase overexpression enhances both cycle progression interleukin 2 production after...
We report the isolation and characterization of a 1994-base-pair cDNA that encompasses entire transcription unit mitochondrial 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase (EC 4.1.3.5.) gene from rat. Analysis nucleotide sequence reveals encodes polypeptide 508 residues 56,918-Da molecular mass. Identify clone isolated as HMG-CoA was confirmed by following criteria: (i) Amino acid are 65% homologous with hamster cytosolic synthase. (ii) 19-amino probably corresponding to...
We have explored the role of mitochondrial 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase in regulating ketogenesis. had previously cloned cDNA for HMG-CoA and now studied regulation vivo expression this gene rat liver. The amount processed mRNA is rapidly changed response to cyclic AMP, insulin, dexamethasone refeeding, greatly increased by starvation, fat feeding diabetes. conclude that one point ketogenic control exercised at level genetic synthase.
Amyloid-β peptide (Aβ) aggregates induce nitro-oxidative stress, contributing to the characteristic neurodegeneration found in Alzheimer's disease (AD). One of most strongly nitrotyrosinated proteins AD is triosephosphate isomerase (TPI) e
// Marc Núnez-Ollé 1 , Carole Jung 2 Berta Terré 3 Norman A. Balsiger Cristina Plata Ramon Roset Carlos Pardo-Pastor Marta Garrido Santiago Rojas 4 Francesc Alameda 1,5 Josep Lloreta 5 Juan Martín-Caballero 6 Juana M. Flores 7 Travis H. Stracker Miguel Valverde Francisco J. Muñoz 2,* and Gabriel Gil-Gómez 1,* Apoptosis Signalling Group, IMIM (Institut Hospital del Mar d'Investigacions Mèdiques), Barcelona, Spain Laboratory of Molecular Physiology, Universitat Pompeu Fabra, Institute for...
Amyloid β-peptide (Aβ) fibril deposition on cerebral vessels produces amyloid angiopathy that appears in the majority of Alzheimer9s disease patients. An early onset a variant called hereditary hemorrhage with amyloidosis Dutch type is caused by point mutation Aβ yielding Aβ<sub>Glu22→Gln</sub>. The present study addresses effect fibrils from both wild-type and mutated vascular cells, as well putative protective role antioxidants angiopathy. For this purpose, we studied cytotoxicity induced...
Multiciliated cells (MCCs) ensure fluid circulation in various organs. Their differentiation is marked by the amplification of cilia-nucleating centrioles, driven a genuine cell-cycle variant, which characterized wave-like expression canonical and non-canonical cyclins such as Cyclin O (CCNO). Patients with CCNO mutations exhibit subtype primary ciliary dyskinesia called reduced generation motile cilia (RGMC). Here, we show that Ccno activated at crossroads onset MCC differentiation, entry...
Multiciliated cells (MCCs) project dozens to hundreds of motile cilia from their apical surface promote the movement fluids or gametes in mammalian brain, airway reproductive organs. Differentiation MCCs requires sequential action Geminin family transcriptional activators, GEMC1 and MCIDAS, that both interact with E2F4/5-DP1. How these factors activate transcription extent which they play redundant functions remains poorly understood. Here, we demonstrate targets proximal proteomes MCIDAS...
Abstract Multiciliated cells (MCC) ensure proper fluid circulation in various organs metazoans. Their differentiation is marked by the massive ampliication of cilia-nucleating centrioles and known to be controlled cell cycle components. Tn a companion study, we show that MCC driven genuine cell-cycle variant characterized sequential wave-like expression canonical non-canonical cyclins such as Cyclin O (CCNO). Patients with CCNO mutations exhibit subtype Primary Ciliary Dyskinesia (PCD)...