A5023941803- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- Pneumocystis jirovecii pneumonia detection and treatment
- Sulfur Compounds in Biology
- Cystic Fibrosis Research Advances
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Diagnosis and treatment of tuberculosis
- Biosensors and Analytical Detection
- Cancer therapeutics and mechanisms
- Infectious Diseases and Tuberculosis
- Bacterial Identification and Susceptibility Testing
- COVID-19 diagnosis using AI
National Institute for Medical Research
2018-2025
Nelson Mandela African Institution of Science and Technology
2022-2025
Mbeya University of Science and Technology
2014-2018
Tuberculosis remains a global health concern, and half of cured patients have permanent lung injury. N-acetylcysteine (NAC) has shown beneficial antimicrobial, antioxidant, immunomodulatory effects in preclinical tuberculosis models. We examined its on treatment outcomes.
<h2>Abstract</h2><h3>Background</h3> Half the global tuberculosis health burden is due to post-tuberculosis lung disease. Host-directed therapies have been proposed reduce this burden. N-acetylcysteine (NAC) provides conditionally essential amino acid cysteine required for synthesis of glutathione, an antioxidant thiol. We recently reported clinical outcomes a trial adjunctive NAC in patients with pulmonary tuberculosis, finding that improved secondary endpoint recovery function. Here we...
ABSTRACT Pyrazinamide (PZA) is a key antituberculosis drug, yet no rapid susceptibility test commercially available. PZA drug testing (DST) was performed directly on sputum samples from 327 patients and compared with the indirect method by using Bactec MGIT 960 system in context of patient screening for participation trial. Compared to standard DST, direct DST successful only 59% cases, but results obtained were highly accurate available faster. Agreement between methods varied 90 100% each...
Abstract Bacterial killing in patients with tuberculosis (TB) relapse was compared to that achieving cure, measured by TB molecular bacterial load assay (TB-MBLA) or mycobacteria growth indicator tube (MGIT) time positivity (TTP). TB-MBLA 4 relapsed significantly different 132 cured after 2 weeks of treatment; MGIT TTP showed a significant difference from week 8.
We evaluated the ability of Xpert(®) MTB/RIF assay to detect Mycobacterium tuberculosis in whole blood children with endemic settings high rates HIV infection.From June 2011 September 2012 we prospectively enrolled symptoms or signs suggestive at three research centres Tanzania and Uganda. After clinical assessment, respiratory specimens were collected for microscopy culture, as well MTB/RIF. Children classified according standardised case definitions.A total 232 evaluated; 14 (6.0%) had...
Mbeya, Tanzania.To develop a new liquid culture method to detect Mycobacterium tuberculosis complex (MTC) in sputum using 2,3-diphenyl-5-thienyl-(2)-tetrazolium (STC), the nitrate reductase assay (NRA) and p-nitrobenzoic acid (PNB).Ninety-three samples collected from 18 patients were decontaminated with N-acetyl-L-cysteine-sodium hydroxide MGIT™ 960 STC-NRA cultures, both presence absence of PNB, an inhibitor MTC growth. The reduction STC by colour change indicated mycobacterial growth; NRA...
Alternative tools are needed to improve the detection of M. tuberculosis (M. tb) in HIV co-infections. We evaluated utility Tuberculosis Molecular Bacterial Load Assay (TB-MBLA) compared lipoarabinomannan (LAM) detect tb urine. Sputum Xpert MTB/RIF-positive patients were consented provide urine at baseline, weeks 2, 8, 16, and 24 treatment for TB-MBLA, culture, LAM. Results with sputum cultures microscopy. Initial tb. H37Rv spiking experiments performed validate tests. A total 63 samples...
Background Xpert® MTB/RIF (Xpert) has suboptimal sensitivity for the diagnosis of pulmonary tuberculosis in children. The next-generation Xpert Ultra assay (Xpert Ultra) is substantially more sensitive than and may allow improved detection paediatric tuberculosis. We evaluated diagnostic accuracy versus Methods From May 2011 to September 2012, children with presumptive were enrolled at two Tanzanian research sites EDCTP-funded TB CHILD project. Sputum samples collected examined using sputum...