A5073277191- Plant Parasitism and Resistance
- Plant and animal studies
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Legume Nitrogen Fixing Symbiosis
- Algal biology and biofuel production
- Plant biochemistry and biosynthesis
- Light effects on plants
- Microbial Natural Products and Biosynthesis
- Allelopathy and phytotoxic interactions
- Insect-Plant Interactions and Control
- Microbial Metabolism and Applications
- Lipid metabolism and biosynthesis
- CRISPR and Genetic Engineering
- Plant and Biological Electrophysiology Studies
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Pesticide and Herbicide Environmental Studies
- American Environmental and Regional History
- Plant nutrient uptake and metabolism
- Weed Control and Herbicide Applications
- Ubiquitin and proteasome pathways
- Plant Diversity and Evolution
The University of Western Australia
2016-2025
ARC Centre of Excellence in Plant Energy Biology
2011-2021
Sichuan Agricultural University
2021
Australian Research Council
2011-2020
University of Nottingham
2004-2017
University of Oxford
2008-2013
Western Australian Marine Science Institution
2011-2012
The University of Queensland
2012
Abstract Chloroplasts of photosynthetic organisms harness light energy and convert it into chemical energy. In several land plants, GOLDEN2-LIKE (GLK) transcription factors are required for chloroplast development, as glk1 glk2 double mutants pale green deficient in the formation apparatus. We show here that Arabidopsis thaliana accumulate abnormal levels chlorophyll precursors constitutive GLK gene expression leads to increased accumulation transcripts antenna proteins biosynthetic enzymes....
Karrikins are butenolides derived from burnt vegetation that stimulate seed germination and enhance seedling responses to light. Strigolactones endogenous butenolide hormones regulate shoot root architecture, the branching of arbuscular mycorrhizal fungi. Thus, karrikins strigolactones structurally similar but physiologically distinct plant growth regulators. In Arabidopsis thaliana, both classes require F-box protein MAX2, it remains unclear how discrete achieved. rice, DWARF14 is required...
Smoke is an important abiotic cue for plant regeneration in postfire landscapes. Karrikins are a class of compounds discovered smoke that promote seed germination and influence early development many plants by unknown mechanism. A genetic screen karrikin-insensitive mutants Arabidopsis thaliana revealed karrikin signaling requires the F-box protein MAX2, which also mediates responses to structurally-related strigolactone family phytohormones. synthetic GR24 trigger similar effects on...
Two α/β-fold hydrolases, KARRIKIN INSENSITIVE2 (KAI2) and Arabidopsis thaliana DWARF14 (AtD14), are necessary for responses to karrikins (KARs) strigolactones (SLs) in (Arabidopsis thaliana). Although KAI2 mediates KARs some SL analogs, AtD14 but not KAR responses. To further determine the specificity of these proteins, we assessed ability naturally occurring deoxystrigolactones inhibit hypocotyl elongation, regulate seedling gene expression, suppress outgrowth secondary inflorescences,...
Significance Strigolactone hormones regulate many plant growth and developmental processes are particularly important in regulating response to nonoptimal conditions. Plants produce a range of bioactive strigolactone-like compounds, suggesting that the biosynthesis pathway is complex. Despite this complexity, only one type enzyme, MORE AXILLARY GROWTH1 (MAX1) cytochrome P450, has been attributed diversity strigolactones. Using transcriptomics reverse genetics, we discovered previously...
Abstract Strigolactones (SLs) are carotenoid-derived plant hormones that regulate shoot branching, secondary growth, root development, and responses to soil phosphate. In Arabidopsis (Arabidopsis thaliana), SL biosynthesis requires the sequential action of two carotenoid cleavage dioxygenases, MORE AXILLARY GROWTH3 (MAX3) MAX4, followed by a cytochrome P450, MAX1. rice (Oryza sativa), plastid-localized protein DWARF27 (OsD27) is also necessary for biosynthesis, but equivalent gene in has not...
In a number of land plants, Golden2-like (GLK) genes encode pair partially redundant nuclear transcription factors that are required for the expression photosynthetic and chloroplast development. As biogenesis depends on close co-operation between plastid compartments, GLK gene function must be dependent tight intracellular control. However, extent to which GLK-mediated development intercellular communication is not known. Here we used sector analysis show proteins operate cell-autonomously...
In Arabidopsis thaliana, the α/β-fold hydrolase KARRIKIN INSENSITIVE2 (KAI2) is essential for normal seed germination, seedling development, and leaf morphogenesis, as well responses to karrikins. KAI2 a paralog of DWARF14 (D14), proposed strigolactone receptor, but evolutionary timing functional divergence between D14 clades has not been established. By swapping gene promoters, we show that proteins are functionally distinct. We catalytic serine function in plants biochemical activity...
Karrikins (KARs) are butenolides found in smoke that can influence germination and seedling development of many plants. The KAR signaling mechanism is hypothesized to be very similar the plant hormone strigolactone (SL). Both pathways require F-box protein MORE AXILLARY GROWTH2 (MAX2), other core components have shared ancestry. Putatively, activates receptor KARRIKIN INSENSITIVE2 (KAI2), triggering its association with E3 ubiquitin ligase complex SCFMAX2 downstream targets SUPPRESSOR OF...
Strigolactone hormones are derived from carotenoids via carlactone, and act through the α/β-hydrolase D14 F-box protein D3/MAX2 to repress plant shoot branching. While MAX2 is also necessary for normal seedling development, known strigolactone biosynthesis genes not, raising question of whether endogenous, canonical strigolactones carlactone have a role in morphogenesis. Here, we report chemical synthesis precursor show that it represses Arabidopsis branching influences leaf morphogenesis...
Summary The control of seed germination in response to environmental conditions is important for plant success. We investigated the role karrikin receptor KARRIKIN INSENSITIVE 2 ( KAI 2) Arabidopsis seeds osmotic stress, salinity and high temperature. Germination kai2 mutant was examined NaCl, mannitol elevated effect on wild‐type seeds, hypocotyl elongation expression karrikin‐responsive genes also such stresses. germinated less readily than more sensitive inhibition by abiotic stress....
Summary Stromules are motile extensions of the plastid envelope membrane, whose roles not fully understood. They present on all types but more common and extensive non‐green plastids that sparsely distributed within cell. During tomato fruit ripening, chloroplasts in mesocarp tissue differentiate into chromoplasts undergo major shifts morphology. In order to understand what factors regulate stromule formation, we analysed biogenesis tobacco hypocotyls two distinct populations mesocarp. We...
KARRIKIN INSENSITIVE 2 (KAI2) is an α/β hydrolase involved in seed germination and seedling development. It essential for plant responses to karrikins, a class of butenolide compounds derived from burnt material that are structurally similar strigolactone hormones. The mechanistic basis the function KAI2 development remains unclear. We have determined crystal structure Arabidopsis thaliana space groups P2(1) 2(1) (a =63.57 Å, b =66.26 c =78.25 Å) =50.20 =56.04 =52.43 β =116.12°) 1.55 2.11 Å...
Karrikins are potent germination stimulants generated by the combustion of plant matter. Treatment Arabidopsis with karrikins triggers a signalling process that is dependent upon putative receptor protein KARRIKIN INSENSITIVE 2 (KAI2). KAI2 homologue DWARF 14 (D14), for endogenous strigolactone hormones. Genetic analyses suggest also perceives signal(s) not strigolactones. Activation addition to plants induces expression transcripts including D14-LIKE (DLK2). We constructed synthetic...