A5007028833- Nanocluster Synthesis and Applications
- Receptor Mechanisms and Signaling
- Polyoxometalates: Synthesis and Applications
- Neuroscience and Neuropharmacology Research
- Chemical Synthesis and Analysis
- Advanced Nanomaterials in Catalysis
- Lipid Membrane Structure and Behavior
- Luminescence and Fluorescent Materials
- Metal-Organic Frameworks: Synthesis and Applications
- Carbon and Quantum Dots Applications
- Neuropeptides and Animal Physiology
- Advanced biosensing and bioanalysis techniques
- Neurotransmitter Receptor Influence on Behavior
- Gold and Silver Nanoparticles Synthesis and Applications
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Lanthanide and Transition Metal Complexes
- Nanoparticle-Based Drug Delivery
- Bacteriophages and microbial interactions
- Nanoplatforms for cancer theranostics
- DNA and Nucleic Acid Chemistry
- Ergonomics and Musculoskeletal Disorders
- Analytical Methods in Pharmaceuticals
- vaccines and immunoinformatics approaches
- Ocular Surface and Contact Lens
Nantong University
2018-2025
Nanjing Medical University
2025
Hebei University
2025
Jilin University
2013-2020
State Key Laboratory of Supramolecular Structure and Materials
2013-2020
Institute of Theoretical Physics
2015-2019
Jilin Medical University
2016-2018
Cornell University
2009
Seattle Reproductive Medicine
2009
Columbia University
1995-2001
The binding site of the β<sub>2</sub>adrenergic receptor, like that other homologous G-protein-coupled receptors, is contained within a water-accessible crevice formed among its seven membrane-spanning segments. Methanethiosulfonate ethylammonium (MTSEA), charged, hydrophilic, lipophobic, sulfhydryl-specific reagent, had no effect on agonist or antagonist to wild-type β<sub>2</sub> receptor expressed in HEK 293 cells. This suggested endogenous cysteines are accessible crevice. In contrast,...
The binding site of the dopamine D2 receptor, like that other homologous G protein-coupled receptors, is contained within a water-accessible crevice formed among its seven membrane-spanning segments. We have developed method to map systematically all residues forming surface this binding-site crevice, and we applied third segment (M3). mutated, one at time, 23 in flanking M3 cysteine expressed mutant receptors heterologously. Ten these mutants reacted with charged, hydrophilic, lipophobic,...
Pillarenes and calixarenes showed obvious inhibition of HPV16 L1 pentamer formation via their selective binding to Arg Lys residues at the monomer interface, which was reversible after release cyclic arenes. are more effective than in terms efficiency, attributing different kinetics affinity.
The binding site of the dopamine D2 receptor, like that other homologous G-protein-coupled receptors, is contained within a water-accessible crevice formed among its seven membrane-spanning segments. Using substituted-cysteine accessibility method, we previously mapped residues form surface binding-site in third and fifth segments (M3 M5). We have now mutated to cysteine, one at time, 26 consecutive flanking seventh segment (M7) expressed mutant receptors HEK 293 cells. Nine these mutants...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTResidues in the Fifth Membrane-Spanning Segment of Dopamine D2 Receptor Exposed Binding-Site CreviceJonathan A. Javitch, Dingyi Fu, and Jiayun ChenCite this: Biochemistry 1995, 34, 50, 16433–16439Publication Date (Print):December 19, 1995Publication History Published online1 May 2002Published inissue 19 December 1995https://pubs.acs.org/doi/10.1021/bi00050a026https://doi.org/10.1021/bi00050a026research-articleACS PublicationsRequest reuse...
In an effort to improve both the stability of virus-like particles (VLPs) and medical activity polyoxometalates (POMs), a new hybrid assembly system between human papillomavirus (HPV) capsid protein L1 europium-containing POM (EuW10) has been constructed, for first time, via electrostatic interactions them. The co-assembly EuW10 HPV 16 L1-pentamer (L1-p) in buffer solution resulted encapsulation POMs cavity VLPs, which was further confirmed by cesium chloride (CsCl) gradient...
Luminescent security printing is of particular importance in the information era. However, use conventional paper still carries a lot economic and environmental issues. Therefore, developing new environmentally friendly material with low cost imperative. To achieve aforementioned goals, novel lanthanide polyoxometalate doped gelatin/glycerol films high transparency, strength, good flexibility have been developed via solution-casting method. The electrostatic interaction between zwitterionic...
Objective: An effective vaginal microbicide against sexual HIV transmission remains elusive, with requirements for adherence to appropriate application of effective, nontoxic products being a major deterrent. We explored methods enable sustained release combinations antiretroviral microbicides, utilizing intravaginal rings composed biosoluble Acacia gum or nonbiodegradable hydrogel 2-hydroxyethyl methacrylate and sodium methacrylate, materials approved use by the US Food Drug Administration....
The antipsychotic drugs have been shown to be inverse agonists at the D(2) dopamine receptor. We examined mechanism of this agonism by making mutations in residue T343 base sixth transmembrane spanning region T343R, T343S and T343K mutant receptors were made T343R characterized detail. receptor exhibits properties a that resides more activated state, namely increased agonist binding affinity (independent G-protein coupling dependent on efficacy), potency functional tests (adenylyl cyclase...
SummarySeveral loci and candidate genes for epilepsies or epileptic syndromes map have been suggested to chromosome 8. We investigated families with adolescent-onset idiopathic generalized epilepsy (IGE), linkage markers spanning The IGEs that we studied included juvenile myoclonic (JME), only tonic-clonic seizures occurring either randomly during the day (random grand mal) on awakening (awakening mal), absence (JAE). looked a gene common all these IGEs, but also specific subforms of IGE....
We propose a unique concept for transforming the liquid-phase fluorometric assay into an enhanced nanopore analysis, which is based on analyte binding-mediated changes in surface chemistry of noble metal nanostructures confined space. In proof-of-concept trial, bovine serum albumin-protected gold nanoclusters (BSA–Au NCs) were designed as sensing unit biothiol determination. Through specific interaction between biothiols and BSA–Au NCs, validation system not only performed well aqueous...
Two hybrid supramolecular complexes comprising magnetic core and dendritic periphery were prepared, which exhibited uniform size, definite molecular weight chemical composition, applicable as enhanced contrast agents.
Gold–silver alloy nanoclusters (AuAgNCs) were synthesized, protected by glutathione S-transferase (GST). The as-prepared GST-AuAgNCs displayed strong (QY = 15.9%) fluorescence with two emission peaks around 477 and 653 nm under excitation of 395 nm. In this system, both the NC core organic shell contributed to development a "turn-on" ratiometric fluorescent probe for sensitively selectively detecting oxytetracycline (OTC). First, green at enhanced addition OTC into GST-AuAgNC solution,...
The binding-induced luminescence enhancement of an Eu-containing polyoxometalate (POM), EuSiWMo, by the arginin/lysine-rich cationic peptides supplied a platform to detect capsid proteins human papillomaviruses (HPVs). However, strong binding affinity between them makes it very difficult be differentiated among from different subtypes HPVs. Therefore, several strategies monitor POM-peptide are performed and finally discriminations on representative HPV achieved in present study. results show...
Au-NCs@GST, a strong red-emitting nanomaterial, was newly fabricated and applied to detect metronidazole in solution living cells.