A5012161440- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Fibroblast Growth Factor Research
- Exercise and Physiological Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Cholesterol and Lipid Metabolism
- Thermoregulation and physiological responses
- Drug Transport and Resistance Mechanisms
- Regulation of Appetite and Obesity
- Biochemical effects in animals
- Amino Acid Enzymes and Metabolism
- Lipid metabolism and biosynthesis
- Muscle metabolism and nutrition
- Diet and metabolism studies
- Contact Dermatitis and Allergies
- Epigenetics and DNA Methylation
- Consumer Attitudes and Food Labeling
- Cellular transport and secretion
- Cardiovascular Disease and Adiposity
- Dietary Effects on Health
- Food Industry and Aquatic Biology
- Microtubule and mitosis dynamics
- Effects and risks of endocrine disrupting chemicals
- Protein Hydrolysis and Bioactive Peptides
- Pharmacogenetics and Drug Metabolism
University of Wisconsin–Madison
2015-2024
Center for Cancer Research
2016
Hungarian Academy of Sciences
2005-2012
Semmelweis University
2012
Institute of Molecular Life Sciences
2005
Hammersmith Hospital
2005
We recently found that feeding healthy mice a diet with reduced levels of branched-chain amino acids (BCAAs), which are associated insulin resistance in both humans and rodents, modestly improves glucose tolerance slows fat mass gain. In the present study, we show BCAA promotes rapid loss without calorie restriction obese mice. Selective reduction dietary BCAAs also restores sensitivity to mice, even as they continue consume high-fat, high-sugar diet. A low transiently induces FGF21...
The induction of beige adipocytes in s.c. white adipose tissue (WAT) depots humans is postulated to improve glucose and lipid metabolism obesity. ability obese, insulin-resistant induce unknown.We exposed lean obese research participants cold (30-minute ice pack application each day for 10 days the upper thigh) or treated them with β3 agonist mirabegron. We determined marker expression by IHC quantitative PCR, we analyzed mitochondrial bioenergetics UCP activity an Oxytherm system.Cold...
Homeostatic temperature regulation is fundamental to mammalian physiology and controlled by acute chronic responses of local, endocrine nervous regulators. Here, we report that loss the heparan sulfate proteoglycan, syndecan-1, causes a profoundly depleted intradermal fat layer, which provides crucial thermogenic insulation for mammals. Mice without syndecan-1 enter torpor upon fasting show multiple indicators cold stress, including activation stress checkpoint p38α in brown adipose tissue,...
Syndecan-1 (Sdc1), a cell surface heparan sulfate proteoglycan normally expressed primarily by epithelia and plasma cells, is aberrantly induced in stromal fibroblasts of breast carcinomas. Stromal fibroblast-derived Sdc1 participates paracrine growth stimulation carcinoma cells orchestrates extracellular matrix fiber alignment, thereby creating migration invasion-permissive microenvironment. Here, we specifically tested the role metastasis. The metastatic potential aggressive mouse mammary...
Abstract Low‐protein (LP) diets are associated with a decreased risk of diabetes in humans, and promote leanness glycaemic control both rodents humans. While the effects an LP diet on mediated by reduced levels branched‐chain amino acids, we have observed that reducing dietary other six essential acids leads to changes body composition. Here, find histidine plays key role response male C57BL/6J mice. Specifically 67% reduces weight gain young, lean mice, adipose mass without altering glucose...
We have developed a rapid, simple and reliable, antibody-based flow cytometry assay for the quantitative determination of membrane proteins in human erythrocytes. Our method reveals significant differences between expression levels wild-type ABCG2 protein heterozygous Q141K polymorphic variant. Moreover, we find that nonsense mutations on one allele result 50% reduction erythrocyte this protein. Since polymorphisms are known to modify essential pharmacokinetic parameters, uric acid...
Abstract Cytosolic citrate is imported from the mitochondria by SLC25A1, and extracellular milieu SLC13A5. In cytosol, used ACLY to generate acetyl-CoA, which can then be exported endoplasmic reticulum (ER) SLC33A1. Here, we report generation of mice with systemic overexpression (sTg) SLC25A1 or Both animals displayed increased cytosolic levels acetyl-CoA; however, SLC13A5 sTg developed a progeria-like phenotype premature death, while did not. Analysis metabolic profile revealed widespread...
Dermal white adipose tissue (dWAT) was recently recognized for its potential to modify whole body metabolism. Here, we show that dWAT can be quantified using a high-resolution, fat-specific magnetic resonance imaging (MRI) technique. Noninvasive MRI has been used describe adipocyte depots many years; the technique uses an advanced method measure thickness of dWAT, together with total volume WAT and relative activation/fat depletion brown tissues (BAT). Since skin-embedded adipocytes may...
Elimination of food calories as heat could help redress the excess accumulation metabolic energy exhibited obesity. Prior studies have focused on induction thermogenesis in beige and brown adipose tissues application this principle, particularly because β-adrenergic environment associated with thermogenic activation has been shown to positive health implications. The counterpoint strategy is regulation loss; we propose that mammals inefficient conservation will require more maintain body...
The GLUT (SLC2) family of membrane-associated transporters are described as glucose transporters. However, this is divided into three classes and, though the regulated transporter activity class I proteins becoming better understood, III protein functions continue to be obscure. We have cataloged relative expression and splicing SLC2 mRNA isomers in tumors normal tissues, with a focus on breast cell lines. for GLUT8 predominant species expressed alongside GLUT1 many but exists mostly an...
Skin has been shown to be a regulatory hub for energy expenditure and metabolism: mutations of skin lipid metabolism enzymes can change the rate thermogenesis susceptibility diet-induced obesity. However, little is known about physiological basis this function. Here we show that thermal properties are highly reactive diet: within three days, high fat diet reduces heat transfer through skin. In contrast, dietary manipulation prevents obesity accelerates loss skins. We found was largest target...
Calorie restriction (CR) promotes healthspan and extends the lifespan of diverse organisms, including mice, there is intense interest in understanding molecular mechanisms by which CR functions. Some studies have demonstrated that induces fibroblast growth factor 21 (FGF21), a hormone regulates energy balance when overexpressed, metabolic health longevity but role FGF21 response to has not been fully investigated. We directly examined physiological diet feeding
Key points Several distinct strategies produce and conserve heat to maintain the body temperature of mammals, each associated with unique physiologies, consequences for wellness disease susceptibility Highly regulated properties skin offset total requirement production We hypothesize that adipose component is primarily responsible modulating flux; here we evaluate relative regulation depots in mouse human, test their recruitment conservation found insulating dermal white tissue accumulates...
Abstract Background The ABCA1 protein plays a pivotal role in reverse cholesterol transport, by mediating the generation of HDL particles and removing cellular cholesterol. Both proper expression plasma membrane internalization along with apoA-I are required for function. Therefore, we developed model system to investigate effect clinically relevant drugs on cell surface appearance ABCA1. Results By retroviral transduction system, established stable mammalian lines expressing functional...
<title>Abstract</title> Skin has been shown to be a regulatory hub for energy expenditure and metabolism: mutations of skin lipid metabolism enzymes can change the rate thermogenesis susceptibility diet-induced obesity. However, little is known about physiological basis this function. Here we show that thermal properties are highly reactive diet: within three days, high fat diet reduces heat transfer through skin. In contrast, dietary manipulation prevents obesity accelerates loss skins. We...
Skin has been shown to be a regulatory hub for energy expenditure and metabolism: mutations of skin lipid metabolism enzymes can change the rate thermogenesis susceptibility diet-induced obesity. However, little is known about physiological basis this function. Here we show that thermal properties are highly reactive diet: within three days, high fat diet reduces heat transfer through skin. In contrast, dietary manipulation prevents obesity accelerates loss skins. We found was largest target...
ABSTRACT Mammalian skin impacts metabolic efficiency system-wide, controlling the rate of heat loss and consequent production. Here we compare unique fat depots associated with mouse human skin, to determine whether they have corresponding function regulation. For human, assay a skin-associated (SAF) body-wide depot distinguish it from subcutaneous pads characteristic abdomen upper limbs. We show that thickness SAF is not related general adiposity; much thicker (1.6-fold) in women than men,...
Abstract Low protein (LP) diets are associated with a decreased risk of diabetes in humans, and low diet promotes leanness glycemic control both rodents humans. While the effects LP on mediated by reduced dietary levels branched- chain amino acids (BCAAs), we have observed that reducing other six essential leads to changes body composition. Here, find histidine plays key role response male C57BL/6J mice. Specifically 67% reduces weight gain young, lean mice, adipose mass gain, without...