A5017034748- Renal and related cancers
- Genetic and Kidney Cyst Diseases
- Birth, Development, and Health
- Renal Diseases and Glomerulopathies
- Urological Disorders and Treatments
- Pediatric Urology and Nephrology Studies
- Cardiovascular Function and Risk Factors
- Organ Donation and Transplantation
- Pregnancy and preeclampsia studies
- HIV Research and Treatment
- Biomarkers in Disease Mechanisms
- Bacteriophages and microbial interactions
- Reproductive Biology and Fertility
- Signaling Pathways in Disease
- Genetic Syndromes and Imprinting
- Diabetes Treatment and Management
- Apelin-related biomedical research
- Renal cell carcinoma treatment
- Reproductive System and Pregnancy
- Genomics and Phylogenetic Studies
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- HIV-related health complications and treatments
- Protease and Inhibitor Mechanisms
- Chemotherapy-induced cardiotoxicity and mitigation
Baylor College of Medicine
2012-2021
Bassett Medical Center
2019
Icahn School of Medicine at Mount Sinai
2002-2013
Johnson University
2002
University of Alabama at Birmingham
1996-1998
Vanderbilt University
1998
University of Alabama
1996
GDNF signaling through the Ret receptor tyrosine kinase (RTK) is required for ureteric bud (UB) branching morphogenesis during kidney development in mice and humans. Furthermore, many other mutant genes that cause renal agenesis exert their effects via GDNF/RET pathway. Therefore, RET believed to play a central role organogenesis. Here, we re-examine extent which functions of Gdnf are unique, by seeking conditions can develop absence. We find absence negative regulator Spry1, Gdnf, no longer...
The metanephric kidney is a mesodermal organ that develops as result of reciprocal interactions between the ureteric bud and blastema. generation embryonic stem (ES) cell-derived progenitors offers potential for regenerative therapies but often limited by development tumor formation. Because brachyury (T) denotes mesoderm specification, mouse ES cell line with green fluorescence protein (GFP) knocked into functional T locus well lacZ in ROSA26 (LacZ/T/GFP) was used selection lineage tracing....
Renal glomerular capillary tufts have been believed to arise from angiogenic ingrowth of extrinsic vessels. We found, however, that when embryonic day 12 (E12) mouse kidneys were maintained in culture for 6 days and then grafted into anterior eye chambers adult transgenic ROSA26 host mice (which carry the beta-galactosidase transgene), endothelial cells within grafts predominantly intrinsic, kidney origin. To identify potential precursors, we immunolabled with antibodies against vascular...
To address origins of glomerular endothelial and mesangial cells in embryonic mammalian kidneys, we established interspecies grafts between rats mice, which fetal kidneys were implanted into the anterior eye chamber adult hosts. After 5-7 days, hosts bearing received intravenous injections with species-specific monoclonal antibodies (MAbs) to matrix components. In all cases, basement membranes matrices labeled solely for donor-derived matrix. Additionally, microvessel extracellular within...
HIV-1 Nef induces podocyte proliferation and dedifferentiation by activating the Stat3 MAPK1,2 pathways. Activation of also occurs in human kidneys affected HIV-associated nephropathy (HIVAN), but its contribution to development HIVAN is unknown. Here, we generated transgenic mice (Tg26) with either 75% activity (Tg26-SA/+) or 25% (Tg26-SA/-). The Tg26-SA/+ mice, not Tg26-SA/- showed increased phosphorylation. phenotype was different from Tg26 developed significantly less proteinuria,...
WT1 encodes a transcription factor involved in kidney development and tumorigenesis. Using representational difference analysis, we identified new set of targets, including homologue the Drosophila receptor tyrosine kinase regulator, sprouty. Sprouty1 was up-regulated cell lines expressing wild-type but not mutant WT1. bound to endogenous sprouty1 promoter vivo directly regulated through an early growth response gene-1 binding site. Expression overlapped developing metanephric mesenchyme,...
The actin depolymerizing factors (ADFs) play important roles in several cellular processes that require cytoskeletal rearrangements, such as cell migration, but little is known about the vivo functions of ADFs developmental events like branching morphogenesis. While molecular control ureteric bud (UB) during kidney development has been extensively studied, detailed underlying this process remain poorly understood. To gain insight into role dynamics renal morphogenesis, we studied functional...
Abstract Understanding the cellular events that underlie epithelial morphogenesis is a key problem in developmental biology. Here, we describe new transgenic mouse line makes it possible to visualize individual cells specifically Wolffian duct and ureteric bud, structures give rise collecting system of kidney. myr‐Venus, membrane‐associated form fluorescent protein Venus, was expressed bud lineage under control Hoxb7 promoter. In Hoxb7/myr‐Venus mice, outlines all are strongly labeled at...
The Notch pathway is an evolutionarily conserved signaling cascade that critical in kidney development and has also been shown to play a pathogenetic role variety of diseases. We have previously the activated human immunodeficiency virus-associated nephropathy (HIVAN) as well rat model disease. In this study, we examined established Tg26 mouse HIVAN. components were distinctly upregulated kidneys these mice immortalized podocytes derived from mice. Notch1 Notch4 glomeruli, was expressed...
The human protein kinase X gene ( PRKX ) is a member of an ancient family cAMP-dependent serine/threonine kinases here shown to be phylogenetically distinct from the classical PKA , PKB / Akt PKC SGK and PKG families. Renal expression developmentally regulated restricted ureteric bud epithelium fetal metanephric kidney. Aberrant adult kidney was found in autosomal dominant polycystic disease. markedly activated migration cultured renal epithelial cells presence cAMP; this effect blocked by...
Human immunodeficiency virus (HIV) infection of kidney cells can lead to HIV-associated nephropathy (HIVAN) and aggravate the progression other chronic diseases. Thus, a better understanding mechanisms HIV-induced cell injury is needed for effective therapy against disease progression. We have previously shown that acetylation activation key inflammatory regulators, NF-κB p65 STAT3, were increased in HIVAN kidneys. Here, we demonstrate role sirtuin 1 (SIRT1) deacetylase regulation STAT3...
Treatment modalities for kidney disease caused by long-term exposure to heavy metals, such as cadmium (Cd), are limited. Often, chronic, environmental metal is not recognized in the early stages; therefore, chelation therapy an effective option. Extracellular vesicles (EVs) derived from stem cells have been demonstrated reduce pathology both acute and chronic models. To test ability of EVs human bone marrow mesenchymal (hBM-MSCs) treat Cd damage, we generated a Cd-exposed medaka model. This...
Autosomal dominant polycystic kidney disease (ADPKD) is a very common inherited caused by mutations in <i>PKD1 </i>or <i>PKD2</i> genes characterized progressive enlargement of fluid-filled cysts and loss renal function [<citeref rid="ref001">1</citeref>]. Previous studies proposed role for human polycystin-1 morphogenesis acting as matrix receptor focal adhesions polycystin-2 putative calcium channel rid="ref002">2</citeref>, <citeref...
The continuing disease burden of HIV-associated nephropathy (HIVAN) warrants better elucidation its pathogenic mechanisms. Given that loss MYH9 function causes a Mendelian renal disease, we hypothesized expression is down-regulated by HIV-1 in HIVAN pathogenesis.Using immunofluorescence, determined glomerular was reduced the kidneys transgenic mice. We further Myh9 podocytes, statistically significantly at protein level, and message level human podocytes transduced with HIV-1. In analyzing...
MYH9 encodes non-muscle myosin heavy chain IIA (NMMHCIIA), the predominant force-generating ATPase in cells. Several lines of evidence implicate a role for podocytopathies. However, NMMHCIIA's function podocytes remains unknown. To better understand this function, we performed immuno-precipitation followed by mass-spectrometry proteomics to identify proteins interacting with NMMHCIIA-enriched actin-myosin complexes. Computational analyses revealed that these belong functional networks...
The human protein kinase X (PRKX) gene was identified previously as a cAMP-dependent serine/threonine that is aberrantly expressed in autosomal dominant polycystic disease kidneys and normally fetal kidneys. PRKX belongs to family phylogenetically functionally distinct from classical A kinases. Expression of activates renal epithelial cell migration tubular morphogenesis culture, suggesting it might regulate branching growth the collecting duct system kidney. With use mouse embryonic kidney...