A5101977164- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Quasicrystal Structures and Properties
- Protein Structure and Dynamics
- Wastewater Treatment and Nitrogen Removal
- Silk-based biomaterials and applications
- Titanium Alloys Microstructure and Properties
- Photoreceptor and optogenetics research
- Catalytic Processes in Materials Science
- Enzyme Structure and Function
- Advanced Fluorescence Microscopy Techniques
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Metallurgical and Alloy Processes
- Water Treatment and Disinfection
- Bacteriophages and microbial interactions
- Neurobiology and Insect Physiology Research
- HIV Research and Treatment
- Metal complexes synthesis and properties
- Biochemical and Structural Characterization
- Hydrogen embrittlement and corrosion behaviors in metals
- Supramolecular Self-Assembly in Materials
- Hedgehog Signaling Pathway Studies
- Glycosylation and Glycoproteins Research
- Hematopoietic Stem Cell Transplantation
Shenyang Aerospace University
2022-2024
Shaoguan University
2022-2024
Guangzhou University
2015-2024
Center for Excellence in Molecular Cell Science
2024
University of Chinese Academy of Sciences
2024
Northeast Institute of Geography and Agroecology
2023
Chinese Academy of Sciences
2023
Fudan University
2022-2023
Shanghai Jiao Tong University
2017-2022
Shanghai Children's Hospital
2017-2022
To investigate the consequences of macromolecular crowding on behavior a globular protein, we performed combined experimental and computational study 148-residue single-domain alpha/beta Desulfovibrio desulfuricans apoflavodoxin. In vitro thermal unfolding experiments, as well assessment native denatured structures, were probed by using far-UV CD in presence various amounts Ficoll 70, an inert spherical agent. 70 has concentration-dependent effect stability apoflavodoxin (DeltaT(m) 20...
A family of proteases called caspases mediate apoptosis signaling in animals. We report a GFP-based fluorogenic protease reporter, dubbed "FlipGFP", by flipping beta strand the GFP. Upon activation and cleavage, is restored, leading to reconstitution GFP fluorescence. FlipGFP-based TEV reporter achieves 100-fold fluorescence change. executioner caspase visualized live zebrafish embryos with spatiotemporal resolution. FlipGFP also apoptotic cells midgut Drosophila. Thus, will be useful for...
Abstract Learning to engineer self-assembly would enable the precise organization of molecules by design create matter with tailored properties. Here we demonstrate that proteins can direct buckminsterfullerene (C 60 ) into ordered superstructures. A previously engineered tetrameric helical bundle binds C in solution, rendering it water soluble. Two tetramers associate one , promoting further revealed a 1.67-Å crystal structure. Fullerene groups occupy periodic lattice sites, sandwiched...
De novo design provides an attractive approach to test the mechanism by which metalloproteins define geometry and reactivity of their metal ion cofactors. While there has been considerable progress in designing proteins that bind transition ions including iron-sulfur clusters, tetranuclear clusters with oxygen-rich environments not accomplished. Here, we describe consisting four Zn
Rationale: Primary and acquired resistance to Smoothened (Smo) inhibitors largely hampered their clinical efficacy.Given the important functions of hedgehog (Hh) pathway in bone formation development, permanent defects growth caused by Smo further restrict use for pediatric tumor patients.Anti-apoptotic Bcl-2 proteins regulate Hh activity engaging a homology (BH) domain sequence found suppressor fused (Sufu).In this study, we tested effect SIAIS361034, Proteolysis Targeting Chimera (PROTAC)...
Endoplasmic reticulum (ER) stress is one of the driving forces ischemia/reperfusion (IR)-induced acute renal failure (ARF). STF-083010, an inhibitor endonuclease activity inositol-requiring enzyme-1 (IRE1), has potential to block initiation a prolonged unfolded protein response (UPR) that stimulated by ER and alleviates impairments due stress. In current study, it was hypothesized STF-083010 capable ameliorating stress-related damages in IR-induced ARF. Rats were administrated with subjected...
Cyclometalated iridium(III) complexes are promising candidates as photosensitizers (PSs) in photodynamic therapy (PDT). The challenge PDT is the selective killing of cancerous cells over neighboring normal cells. In this work, a pH-responsive cyclometalated complex (probe 1) was designed and synthesized an effective PS to selectively kill cells, with 3-(2-pyridyl)benzaldehyde used main ligand 1-(2-pyridyl)-β-carboline ancillary ligand. Probe 1 shows enhanced photoluminescence emission higher...