Description of Folin Ciocalteau ReagentThe Folin-Ciocalteau (F-C) method assay is the simplest available for measurement phenolic content in products.It a development Denis reagent used early 19th century determination tyrosine proteins [1].F-C can be prepared by dissolving 100 g sodium tungstate (VI) dihydrate and 25 molybdate with 700 ml distilled water, concentrated hydrochloric acid, 50 85% phosphoric acid to which added 150 lithium sulphate hydrate.This very stable if protected from...

Chocolate today is often viewed as a food or snack with little nutritional value. The high saturated fat content of chocolate has also contributed to the belief that its consumption increases risk heart disease. However, recent human studies have proven beneficial effects on some pathogenic mechanisms disease such endothelial function and blood pressure. Although antioxidant properties been known for time, there no examination place in U.S. diet source antioxidants. This paper demonstrates...

This study evaluates the antioxidant capacity of 14 herbs/spices from Cameroon. Freeze-dried samples extracted in methanol (free or unconjugated polyphenol) and 1.2 M hydrochloric acid (HCl) (total that is both conjugated) were analyzed using two different assay methods [Folin−Ciocalteu reagent (Folin) ferric reducing power (FRAP)]. The HCl extracts had significantly higher (P < 0.001) capacities than methanolic extract. Generally, FRAP values Folin values. Although a significant correlation...

Alzheimer’s disease is first characterised by memory loss related to the central cholinergic system alteration. Available drugs provide symptomatic treatment with known side effects. The present study aimed evaluate properties of Carissa edulis aqueous extract on a Scopolamine mouse model as an attempt search for new compounds against disease-related impairment. Memory impairment was induced administration 1 mg/kg (i.p.) 7 days, and mice were treated extract. Behavioural studies performed...

Abstract Diarrhoea is a public health problem in developing countries. It therefore important and useful to identify plants with antidiarrhoeal activity. Alchornea cordifolia quoted by many traditional healers as plant this The activity of its leaf extract was investigated against castor oil induced diarrhoea mice, using morphine the standard reference drug. A significant ( p &lt; 0.01) dose related (100 mg/kg, 200 400 800 mg/kg) A. ethanol observed mg/kg being most effective. delayed mouse...

Once considered a problem of developed countries, obesity and obesity-related complications (such as metabolic syndrome) are rapidly spreading around the globe. The purpose present study was to investigate use Cissus quadrangularis formulation in management syndrome, particularly weight loss central obesity. randomized, double-blind, placebo-controlled design involving 123 overweight obese persons (47.2% male; 52.8% female; ages 19–50). 92 (BMI >30) participants were randomized into three...

To evaluate the effects of two formulations, Cissus quadrangularis-only and a quadrangularis/Irvingia gabonensis combination, on weight loss in overweight obese human subjects. The study was 10 week randomized, double-blind, placebo-controlled design involving 72 or participants (45.8% male; 54.2% female; ages 21–44; mean age = 29.3). were randomly divided into three equal (n 24) groups: placebo, quadrangularis-only, combination. Capsules containing placebo active formulations administered...

The haematinic activity of an orally administered aqueous extract Hibiscus cannabinus leaves was studied on haemolytic anaemic rats. Anaemia induced by oral administration phenylhydrazine for a period 8 days. Red blood cell count, haemoglobin concentration, and pack volume were analysed as indices anaemia. mean haemoglobin, concentration calculated accordingly. Phenylhydrazine significant decrease (P H. may have properties. Key words: Haematinic activity, , anaemia, phenylhydrazine. African...

Obesity is generally linked to complications in lipid metabolism and oxidative stress. The aim of this study was compare the effect a proprietary extract Cissus quadrangularis (CQR-300) that formulation containing CQR-300 (CORE) on weight, blood lipids, stress overweight obese people. first part investigated vitro antioxidant properties CORE using 3 different methods, while second double-blind placebo controlled design, involving initially 168 persons (38.7% males; 61.3% females; ages...

Extracts of 42 medicinal plants used for the treatment anaemia, diabetes, AIDS, malaria and obesity were screened phytochemical substances antioxidant potentials. The plant extracts prepared as hydrolysed (for total antioxidant) non-hydrolysed free antioxidant). analysed using three different assay methods analysis: Folin (Folin Ciocalteu reagent), FRAP (Ferric Reducing Antioxidant Power) DPPH (1,1-diphenyl-2-picrylhydrazyl). leaves Alchornea cordifolia showed highest properties determined...

The emerging resistance of Plasmodium species to currently available anti-malarials remains a public health concern, hence the need for new effective, safe and affordable drugs. Natural products remain reliable source Nefang is polyherbal anti-malarial Cameroonian folklore medicine with demonstrated in vitro antiplasmodial antioxidant activities. It composed Mangifera indica (bark leaf), Psidium guajava, Carica papaya, Cymbopogon citratus, Citrus sinensis, Ocimum gratissimum (leaves). This...

Antiretroviral therapy (ART) has revolutionized the lives of people living with HIV/AIDS by overall improving their quality life and increasing expectancy. However, ART-associated hepatotoxicity metabolic disorders in patients are growing concerns to clinicians, especially due long-term use drugs. This study reported on phytochemical pharmacological profile hydroethanolic extracts Piper nigrum stem (PNS) evaluated its protective effect against tenofovir/lamivudine/efavirenz (TLE)-induced...

<title>Abstract</title> The current study investigates the biological effects of <italic>Costus afer</italic> (CAL and CAS extracts), a plant with known antidiabetic properties used in traditional medicine West tropical Africa, on pathways involved type 2 diabetes mellitus (T2DM) human adipocyte (SW 872) hepatocyte (HepG2) cell models. cytotoxicity CAL extracts was assessed using MTS assay, while their influence glucose uptake HepG2 SW 872 cells triglyceride accumulation oleic...

Aims: This study aimed to investigate the in vivo antianemic activity and vitro antiplasmodial antibacterial activities of beverage fractions from leaves Justicia secunda. Methodology: The was prepared according traditional method further partitioned using dichloromethane, ethyl acetate, methanol. firstly subjected determination solute content mineral analysis. After that, for assay, anemia induced rats by intraperitoneal administration phenyl hydrazine at dose 40 mg kg−1 per day two days....

The over-stimulation of N-methyl-d-aspartate glutamate receptors causes an excitotoxic neuronal death which plays important role in many neurodegenerative diseases. Carissa edulis, a medicinal plant used African pharmacopeia has been shown to have therapeutic effects. In this study, the effects edulis aqueous extract on L-glutamic acid-induced neurotoxicity mice were investigated. Two-month-old received intraperitoneal injection acid (2 g/kg) for seven consecutive days and treated with...

This study investigated the anti-atherogenic effects of Plectranthus glandulosus leaves aqueous, hydro-ethanolic extracts, and ethyl acetate fraction at doses 100, 200, 400 mg/kg for 21 d. As results, dose mg/kg, significantly (p < .001) decreased level total cholesterol (112.52 ± 1.21 mg/dL), triglyceride (76.47 0.97 LDL-C (22.01 2.92 mg/dL). Whereas, a significant increase was observed in HDL-C (74.97 1.99 Moreover, atherogenic index (0.008 0.00 while percentage protection increased (99.13...

Background: Nosocomial infections, particularly sepsis, represent a major public health challenge, especially in hospital settings where hygiene practices may fall short. The use of mobile phones by medical staff can act as vector for pathogen transmission, thereby increasing the risk cross-infections. This study aims to evaluate when using at Yaoundé General Hospital and assess their impact on infection sepsis prevention. Materials methods: A cross-sectional, analytical, evaluative was...

The excessive and inappropriate use of antibiotics promotes the emergence bacterial resistance, prompting research to explore therapeutic alternatives, such as essential oils, due their bioactive substances. However, potential for future resistance oils concerns scientific community. In this context, present study was designed compare responses bacteria exposed sub-inhibitory concentrations (EOs) antibiotics. We assessed antibacterial activity EOs reference using liquid microdilution method....

Four plants (Amphimas pterocaroides, Harungana madagascariensis, Myrianthus arboreus, and Cussonia barteri) that are commonly used in Cameroon for the management or reversal of anaemia were screened their phytochemical content antioxidant potential. extracts (methanolic, hydro-ethanolic, aqueous, hydrolysed) from each prepared analysed. Qualitative tests to detect presence alkaloids, tannins, saponins, flavonoids, glycosides phenols, while three quantitative methods; Folin, Ferric Reducing...

In Cameroon, local plants are traditionally used as remedies for a variety of ailments. this regard, several papers report health benefits Cameroonian spices, which include antioxidant and anti-microbial properties, whereas gastric anti-inflammatory activities have never been previously considered. The present study investigates the hydro-alcoholic extracts eleven spices in epithelial cells (AGS GES-1 cells). showed properties cell-free system reduced H2O2-induced ROS generation cells. After...