In chickens, elevated environmental temperature reduces food intake. We have previously reported that, during heat stress, the intestinal mucosa has an increased capacity to take up sugars. To investigate whether effects of warm environment on sugar uptake are adaptation lower energy intake or a response attributable we examined glucose transport kinetics apical and basolateral membranes jejunum mucosal morphology broiler chickens maintained in climatic chambers for 2 wk. Experimental groups...

Abstract In animal models of inflammation and in farm animals, dietary inclusion spray-dried porcine plasma (SDP) reduces mucosal inflammation. Here, we study whether these effects could be mediated by changes the intestinal microbiota if are similar to those induced oral antibiotics. Weaned 21-day-old C57BL/6 mice were divided into 3 groups: CTL group, fed control diet; COL administered low doses neomycin colistin; SDP supplemented with 8% SDP. After 14 days, analysis fecal microbiome...

Spray-dried preparations from porcine and bovine plasma can alleviate mucosal inflammation in experimental models improve symptoms patients with enteropathy. In rodents, dietary supplementation spray-dried (SDP) attenuates intestinal improves the epithelial barrier function during induced by Staphylococcus aureus enterotoxin B (SEB). The aim of this study was to discern molecular mechanisms involved anti-inflammatory effects SDP. Male C57BL/6 mice were fed 8% SDP or control diet (based on...

Dietary supplementation with spray-dried porcine plasma (SDP) reduces the Alzheimer’s disease (AD) hallmarks in SAMP8 mice. Since gut microbiota can play a critical role AD progression, we have studied if neuroprotective effects of SDP involve microbiota−gut−brain axis. Experiments were performed on two-month-old mice fed standard diet and six-month-old control or an 8% supplemented for four months. Senescence impaired short- long-term memory, reduced cortical brain-derived neurotrophic...

The kinetic parameters of hexose uptake by the small and large intestine chicken have been determined in vitro. Rates initial influx alpha-methyl-D-glucoside L-glucose were measured everted sleeves duodenum, jejunum, ileum, proximal cecum, rectum. Results show following. 1) Maximal transport capacity values for that jejunum is segment best suited Na(+)-mediated uptake. 2) calculated apparent Michaelis constant (in mmol/l) 11.6 7.8 3.5 2.4 7.1 This suggests that, with exception rectum,...

Increased life expectancy has promoted research on healthy aging. Aging is accompanied by increased non-specific immune activation (inflammaging) which favors the appearance of several disorders. Here, we study whether dietary supplementation with spray-dried animal plasma (SDP), been shown to reduce gut-associated lymphoid tissue (GALT) in rodents challenged S. aureus enterotoxin B (SEB), and can also prevent effects aging system homeostasis. We first characterized GALT a mouse model...

Alzheimer’s disease (AD) is characterized by the aberrant processing of amyloid precursor protein (APP) and accumulation hyperphosphorylated tau, both which are accompanied neuroinflammation. Dietary supplementation with spray-dried porcine plasma (SDP) has anti-inflammatory effects in inflammation models. We investigated whether dietary SDP prevents neuropathological features AD. The experiments were performed 2- 6-month-old SAMP8 mice fed a control diet, or diet supplemented 8% SDP, for 4...

Dietary supplementation with spray-dried porcine plasma (SDP) can modulate the immune response of gut-associated lymphoid tissue. SDP reduces acute mucosal inflammation, as well chronic inflammation associated aging. The aim this study was to analyze if could ameliorate colitis in a genetic mouse model inflammatory bowel disease (IBD). Wild-type mice and Mdr1a knockout (KO) were administered control diet or an SDP-supplemented from day 21 (weaning) until 56. histopathological index,...

The specific role of vasopressin in colonic crypt function and its possible synergistic action with aldosterone were studied. Sprague-Dawley rats fed a high-Na+ (HS; 150 mM NaCl) or low-Na+ (LS; microM diet deprived water infused vasopressin, some animals treated receptor subtype V1 V2 antagonists. expression the epithelial Na+ channel (ENaC), alpha-smooth muscle actin (alpha-SMA) aquaporin-2 (AQP-2) determined by immunolocalization distal mucosa. pericryptal concentration was confocal...

Senescence is characterized by an aggravated inflammatory state that reduces vaccine responsiveness. Dietary supplementation with spray-dried porcine plasma (SDP) exerts anti-inflammatory effects in different mucosal areas. We aimed to determine if the properties of SDP improve efficiency immunization senescent animals.

In vivo studies show that raised aldosterone (Aldo) during low-Na adaptation regulates the growth of pericryptal myofibroblasts and reduces permeability colonic epithelium. The aim this study was to reproduce in vitro condition increased Aldo using human CCD-18Co T84 epithelial cells measure myofibroblast proliferation expression intercellular junction proteins. Proliferation quantified by measuring 5-bromo-2'-deoxyuridine incorporation. EGF, VEGFa, transforming factor-β1 (TGF-β1) measured...

Transmural potential difference (PD), short-circuit current (Isc), and electrical resistance (R) were measured in the isolated mucosa of duodenum, jejunum, ileum, proximal cecum, rectum order to characterize properties chicken small large intestine. The intestine was classified into three categories, regarding its characteristics: 1) with four five times higher R than other segments lowest PD; 2) group formed by high PD low R; 3) rectum, R. In all segments, addition D-glucose luminal side...

We examined the characteristics of amino acid and sugar absorption by proximal cecum (PC) chickens during posthatch development. Rates L-proline (Pro) alpha-methyl-D-glucoside (MG) were measured at 2 days, 5 wk, 13 wk after hatch with an in vitro everted-sleeve method. For each age, pieces PC midjejunum incubated solutions containing 0.1-50 mM Pro or MG, active passive components MG determined. Five conclusions may be stated. 1) There are two carrier-mediated transport systems for PC: a...

We have studied the effects of aging on fecal microbiota composition in senescence-accelerated prone mice SAMP8 strain. compared animals two, four, and six months old. Feces were collected at end each period a genomic study was carried out DNA using Illumina MiSeq analyzer. The Shannon diversity index showed similar values along this number species neither affected by aging. phylum Verrucobacteria went up with age, showing seven-fold increase months, to two-month old mice. At family level,...