A5037068261- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Amino Acid Enzymes and Metabolism
- RNA Research and Splicing
- Drug Transport and Resistance Mechanisms
- Microtubule and mitosis dynamics
- Biochemical Analysis and Sensing Techniques
- Metabolism and Genetic Disorders
- Plant Molecular Biology Research
- Animal Nutrition and Physiology
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Mast cells and histamine
- Plant nutrient uptake and metabolism
- Nuclear Structure and Function
- Phosphodiesterase function and regulation
- Peroxisome Proliferator-Activated Receptors
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Phytase and its Applications
- Molecular Sensors and Ion Detection
- Receptor Mechanisms and Signaling
- Lipid Membrane Structure and Behavior
Institute for Research in Biomedicine
2006-2025
Institute of Science and Technology
2025
Institut de Biologia Molecular de Barcelona
2003-2024
Barcelona Institute for Science and Technology
2017-2019
Consejo Superior de Investigaciones Científicas
2009-2010
Universitat de Barcelona
1996-2003
Centromere identity is determined by the formation of a specialized chromatin structure containing centromere-specific histone H3 variant CENP-A. The precise molecular mechanism(s) accounting for specific deposition CENP-A at centromeres are still poorly understood. Centromeric CENP-A, which independent DNA replication, might involve assembly complexes and/or interactions with kinetochore components. However, transiently expressed incorporates throughout indicating that nucleosomes can also...
Abstract During early embryogenesis the zygotic genome remains transcriptionally silent and expression relies on maternally deposited products. Maternal deposition of histones is crucial to preserve chromatin integrity during embryo development, when number nuclei exponentially increases in absence expression. In Drosophila embryo, are as both proteins mRNAs. Histone transcripts only nonpolyadenylated cellular They contain a highly conserved 3′UTR stem-loop structure, which recognized by...
Chromosome pairing constitutes an important level of genome organization, yet the mechanisms that regulate in somatic cells and impact on 3D chromatin organization are still poorly understood. Here, we address these questions Drosophila, organism with robust pairing. In preferentially occurs at loci consisting numerous architectural protein binding sites (APBSs), suggesting a role proteins (APs) regulation. Amongst these, anti-pairing function condensin II subunit CAP-H2 is well established....
Abstract Barrier-to-Autointegration Factor (BAF) is a conserved nuclear envelope (NE) component that binds chromatin and helps its anchoring to the NE. Cycles of phosphorylation dephosphorylation control BAF function. Entering mitosis, releases from facilitates NE-disassembly. At mitotic exit, PP2A-mediated restores binding nucleates NE-reassembly. Here, we show in Drosophila small fraction (cenBAF) associates with centromeres. We also find PP4 phosphatase, which recruited centromeres by...
The intestinal transport of L‐methionine has been investigated in brush‐border membrane vesicles isolated from the jejunum 6‐week‐old chickens. L‐Methionine influx is mediated by passive diffusion and Na + ‐dependent ‐independent carrier‐mediated mechanisms. In absence , cis‐ inhibition experiments with neutral cationic amino acids indicate that two components are involved influx: one sensitive to L‐lysine other 2‐aminobicyclo[2.2.1]heptane‐2‐carboxylic acid (BCH). L‐lysine‐sensitive flux...
The influx ofl-lysine into apical vesicles from the chicken jejunum occurs through two systems, one with low Michaelis constant ( K m ) and features of system b 0,+ other relatively high forl-lysine properties y + . In present study effect a lysine-enriched diet (Lys, containing 68 g l-lysine/kg dietary protein, control animals 48 g/kg) onl-lysine uptake both transport systems was investigated. Results show that 1) lysine enrichment had no on either body weight or efficiency food...
Centromere identity is determined by the specific deposition of CENP-A, a histone H3 variant localizing exclusively at centromeres. Increased CENP-A expression, which frequent event in cancer, causes mislocalization, ectopic kinetochore assembly and genomic instability. Proteolysis regulates expression prevents its misincorporation across chromatin. How proteolysis restricts localization to centromeres not well understood. Here we report that, Drosophila, CENP-ACID levels are regulated...
Chromatin-associated RNAs (cRNAs) are a poorly characterized fraction of cellular that co-purify with chromatin. Their full complexity and the mechanisms regulating their packaging chromatin association remain understood. Here, we address these questions in Drosophila. We find cRNAs constitute heterogeneous group RNA species is abundant heterochromatic transcripts. show interact nuclear ribonucleoproteins (hnRNP) hrp36/hrp48 depletion linker histone dH1 impairs this interaction. induces...
In the chicken intestine,l-methionine is transported by systems that are specific for neutral amino acids (L- and B-like) can also transport cationic (y + m b 0,+ -like). These four uptake pathways have been investigated in brush-border membrane vesicles from jejunum of chickens fed a diet enriched with 0.4%l-methionine. Methionine supplementation 1st to 6th wk age has no effect on body weight or efficiency food utilization. The kinetic analysis l-methionine influx across shows, system L,...
In this paper, the human phosphodiesterase 7A1 (hPDE7A1) promoter region was identified and functionally characterized. Transient transfection experiments indicated that a 2.9 kb fragment of hPDE7A1 5′-flanking region, to position −2907, has strong activity in Jurkat T-cells. Deletion analysis showed proximal up −988, contains major cis-regulatory elements promoter. This minimal regulatory CpG island which is essential for activity. The three potential cAMP-response-element-binding protein...
Background Centromere identity is determined epigenetically by deposition of CenH3, a centromere-specific histone H3 variant that dictates kinetochore assembly. The molecular basis the contribution CenH3 to centromere/kinetochore functions is, however, incompletely understood, as its interactions with rest components remain largely uncharacterised at molecular/structural level. Principal Findings Here, we report on Drosophila CenH3CID recruitment BubR1, conserved protein core component...
Abstract Accumulating evidence hints heterochromatin anchoring to the inner nuclear membrane as an upstream regulatory process of gene expression. Given that formation neural progenitor cell lineages and subsequent maintenance postmitotic neuronal identity critically rely on transcriptional regulation, it seems possible development cells is influenced by type-specific and/or context-dependent programmed regulation anchoring. Here, we explored this possibility genetically disrupting...