Tony Ito-Cole
A5087781969- Single-cell and spatial transcriptomics
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- Retinal Development and Disorders
- Venomous Animal Envenomation and Studies
- Rabies epidemiology and control
- Bacillus and Francisella bacterial research
- Gene Regulatory Network Analysis
- Epigenetics and DNA Methylation
Salk Institute for Biological Studies
2016-2021
Monosynaptic rabies virus tracing is a unique and powerful tool used to identify neurons making direct presynaptic connections onto of interest across the entire nervous system. Current methods utilize complementation glycoprotein gene-deleted SAD B19 strain with its glycoprotein, B19G, mediate retrograde transsynaptic spread single synaptic step. In most conditions, this method labels only fraction input would thus benefit from improved efficiency spread. Here, we report newly engineered...
Abstract Neuronal cell types are classically defined by their molecular properties, anatomy and functions. Although recent advances in single-cell genomics have led to high-resolution characterization of type diversity the brain 1 , neuronal often studied out context anatomical properties. To improve our understanding relationship between features that define cortical neurons, here we combined retrograde labelling with single-nucleus DNA methylation sequencing link neural epigenomic...
ABSTRACT We report the generation of a multimodal cell census and atlas mammalian primary motor cortex (MOp or M1) as initial product BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved morphological electrophysiological properties, cellular resolution input-output mapping, integrated through cross-modal computational analysis. Together, our results advance...