A5014500899- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Urticaria and Related Conditions
- Eosinophilic Disorders and Syndromes
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Autoimmune and Inflammatory Disorders Research
- Galectins and Cancer Biology
- Phytochemistry and biological activities of Ficus species
- Toxin Mechanisms and Immunotoxins
- Cell Adhesion Molecules Research
- Vector-borne infectious diseases
- Mast cells and histamine
- Food Allergy and Anaphylaxis Research
- Virus-based gene therapy research
- Vitamin D Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
Hospital General de México
1999-2025
Universidad Nacional Autónoma de México
2013-2025
Universidad Autónoma de la Ciudad de México
2017
Abstract Leishmania spp. cause a wide range of human diseases, localized skin lesions, mucocutaneous and visceral infections. In the present study, aim was to investigate potential role sanguinarine as specific inhibitor PP2C that can induce apoptosis in parasite. The results demonstrated inhibits, dose-dependent mode at 72 h, growth phosphatase activity both major mexicana promastigotes. Therefore, all assays were performed from this time period onwards. TUNEL assay used identify indicated...
Leishmania mexicana (Lm) causes localized (LCL) and diffuse (DCL) cutaneous leishmaniasis. DCL patients have a poor cellular immune response leading to chronicity. It has been proposed that CD8 T lymphocytes (CD8) play crucial role in infection clearance, although the of cytotoxicity disease control not elucidated. Lesions shown harbor low numbers CD8, as compared with LCL, leishmanicidal treatment restores numbers. The marked towards parasites led us analyze possible functional differences...
Leishmania mexicana can cause both localized (LCL) and diffuse (DCL) cutaneous leishmaniasis, yet little is known about factors regulating disease severity in these patients. We analyzed if the was associated with single nucleotide polymorphisms (SNPs) IL-1β (−511), CXCL8 (−251) and/or inhibitor IL-1RA (+2018) 58 Mexican mestizo patients LCL, 6 DCL 123 control cases. Additionally, we vitro production of by monocytes, expression this cytokine sera patients, as well tissue distribution number...
Pentavalent antimonials are the first-line treatment for localized cutaneous leishmaniasis. However, they have disadvantages such as their elevated toxicity, high costs, and parenteral application. Plant-derived compounds may be an alternative against this disease. Previous in vitro studies shown that (3S)-16,17-didehydrofalcarinol (1), a polyacetylene oxylipin isolated from Tridax procumbens, is active Leishmania mexicana. We analyzed mechanism of action compound 1, evaluating reactive...
Leishmania mexicana causes localized (LCL) or diffuse cutaneous leishmaniasis (DCL). The cause of dissemination in DCL remains unknown, yet NK cells possibly play a role activating leishmanicidal mechanisms during innate and adaptive immune responses. We had previously shown that lipophosphoglycan (LPG) is ligand for TLR2, human cells. have now analyzed LCL patients. numbers effector differed drastically between both groups patients: patients showed reduced cell numbers; diminished IFN-γ...
NKT cells have been associated with protection against Leishmania donovani, yet their role in infections mexicana has not addressed, nor the activation pathway defined after stimulation lipophosphoglycan (LPG). We analyzed of and cytokine production response to LPG. Additionally we compared NKT-cell numbers profile lymph nodes skin lesions induced by BALB/c C57BL/6 mice. show that LPG activates primarily through indirect pathway, initiating TLR2 dendritic (DC), thereby enhancing TLR2, MHC...
Summary Two patients with diffuse cutaneous leishmaniasis caused by Leishmania mexicana were treated two leishmanicidal drugs (pentamidine and allopurinol) combined recombinant interferon‐γ restoring Th‐1 favouring conditions in the patients. Parasites decreased dramatically lesions macrophages diminished concomitantly, while IL‐12‐producing Langerhans cells interferon‐γ– producing NK CD8 + lymphocytes increased a reciprocal manner. The CD4+/CD8 ratio peripheral blood normalized. During...
Eosinophils are mainly associated with parasitic infections and allergic manifestations. They produce many biologically active substances that contribute to the destruction of pathogens through degranulation microbicidal components inflammatory tissue effects. In leishmaniasis, eosinophils have been found within infiltrate protective immunity against parasite. We analyzed responses from patients localized (LCL) diffuse (DCL) cutaneous as well healthy subjects, when exposed Leishmania...
CD43 is an abundant cell surface sialoglycoprotein implicated in hemopoietic adhesion and activation. Cell stimulation through results recruitment of different signaling proteins, including members the Src family kinases, Syk, phospholipase Cgamma2, adapter protein Shc, guanine nucleotide exchange factor Vav, activation kinase C. In this study, we report that human T lymphocytes, zeta-chain part pathway. Upon engagement, was tyrosine-phosphorylated, generating docking sites for...
Summary A strong sex‐associated susceptibility towards Leishmania has been reported in males, yet little is known on the effect of hormones physiopathogenicity. Due to enhanced males mexicana infections , we were interested analysing exerted by main androgen produced ( DHT ) L. promastigotes. Thus, aim this study was assess regulation dihydrotestosterone replication, infectivity, survival and development tissue lesions. Experiments included growth curves promastigotes incubated with...
Mast cells (MCs) play a crucial role during Leishmania infections, which is transmitted through the bite of an infected sand fly that injects saliva together with parasite. Sand complex fluid modulates host immune response. In addition, hormonal factors modulate response and alter susceptibility to infections. Thus, assess impact male sex hormones on mast-cell (MC) we orchiectomized mice, them parasite in presence salivary proteins, analyzed inflammatory MCs. Our results showed MC vector...
Parasites have been engineered to express fluorescent reporter proteins, yet the impact of red proteins on Leishmania infections remains largely unknown. We analysed infection outcome mexicana parasites for constitutive expression mKate protein and evaluated their immunogenicity in BALB/c mice. Infection mice with transfected L. (LmexmKate ) caused enlarged lesion sizes, leading ulceration, containing more parasites, as compared LmexWT . The showed immunogenic properties inducing antibody...