A5039192177- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Nematode management and characterization studies
- T-cell and Retrovirus Studies
- Viral Infections and Vectors
- Plant Virus Research Studies
- Entomopathogenic Microorganisms in Pest Control
- Business, Innovation, and Economy
- Parasite Biology and Host Interactions
- Occupational Health and Safety in Workplaces
- Plant Disease Management Techniques
- Virus-based gene therapy research
- Virology and Viral Diseases
- Organizational Management and Innovation
- Plant Pathogens and Fungal Diseases
- Insects and Parasite Interactions
- Insect Resistance and Genetics
- Mediterranean and Iberian flora and fauna
- Plant and soil sciences
- Higher Education and Sustainability
- Philosophical Thought and Analysis
- Biological Control of Invasive Species
Centro de Investigación en Sanidad Animal
2014-2024
European Union Satellite Centre
2015-2024
Consejo Superior de Investigaciones Científicas
2024
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
1990-2024
Ministry of Agriculture Livestock and Fisheries
2022
National Veterinary Research Institute
2014
European Union
2012
Centro Regional de Selección y Reproducción Animal
2010-2011
Hospital Universitario Fundación Alcorcón
2008
Central University Hospital of Asturias
2008
African swine fever virus (ASFV) was first reported in eastern Europe/Eurasia 2007. Continued spread of ASFV has placed central European countries at risk, and 2014, detected Lithuania Poland. Sequencing showed the isolates are identical to a 2013 from Belarus but differ isolated Georgia
African swine fever (ASF) is an important emerging transboundary animal disease (TAD), which currently has impact on many countries in Africa, Eastern Europe, the Caucasus and Russian Federation. The current situation Europe shows ability of virus to rapidly spread, stands threaten global industry. At present, there no viable vaccine minimize spread stamping out main source control. In February 2011, Ethiopia had reported its first suspected outbreaks ASF. Genomic analyses collected ASF...
ABSTRACT This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as routine diagnostic test ASFV in surveillance, control, eradication programs. A confirmatory the specificity this based on restriction endonuclease analysis was also developed.
A highly sensitive and specific real-time PCR method was developed for the reliable rapid detection of African swine fever virus (ASFV). The uses a commercial Universal Probe Library (UPL) probe combined with specifically designed primer set to amplify an ASFV DNA fragment within VP72 coding genome region. range optimized UPL technique confirmed by analysis large panel (n = 46) isolates, belonging 19 22 viral p72 genotypes described. No amplification signal observed when closely clinically...
This study represents a complete comparative analysis of the most widely used African swine fever (ASF) diagnostic techniques in European Union (EU) using field and experimental samples from animals infected with genotype II ASF virus (ASFV) isolates circulating Europe. To detect ASFV, three different PCRs were evaluated parallel 785 samples. The results showed almost perfect agreement between Universal ProbeLibrary (UPL-PCR) real-time (κ = 0.94 [95% confidence interval {CI}, 0.91 to 0.97])...
A non-haemadsorbing (non-HAD) ASF virus (ASFV) genotype II, namely Lv17/WB/Rie1, was isolated from a hunted wild boar in Latvia 2017. Domestic pigs experimentally infected with the non-HAD ASFV developed nonspecific or subclinical form of disease. Two months later, these animals were fully protected when exposed to other domestic related virulent HAD II ASFV.
An experimental infection was conducted to evaluate horizontal transmission, clinical, virological and humoral response induced in domestic pigs infected with African swine fever (ASF) genotype II virus circulating 2014 into the European Union (EU). Ten naive were placed contact eight experimentally inoculated Lithuanian LT14/1490 ASF (ASFV) responsible for first case detected wild boar Lithuania January 2014. Clinical examination rectal temperature recorded each day. Blood sampling from...
African swine fever (ASF) has persisted in Eastern Europe since 2007, and two endemic zones have been identified the central southern parts of Russian Federation. Moderate- to low-virulent ASF virus isolates are known circulate ASF-affected regions. To improve our knowledge transmission animals recovered from infection, an experimental vivo study was carried out. Four domestic pigs were inoculated with NH/P68 virus, previously characterized develop a chronic form ASF. Two additional...
African swine fever (ASF), the most significant threat to pig industry worldwide, has spread more than 55 countries on three continents, and it affects 77% of world population. In European Union, wild boar (Sus scrofa) is severely affected host. The main reasons for unprecedented constant ASF in Europe are trade activities, continuous movement infected-wild populations among regions lack vaccine prevent infection. this study, we demonstrate that oral immunization with a non-hemadsorbing,...
African swine fever (ASF) is a contagious viral disease of pigs and wild boar that poses major threat to the global industry. The genotype II virus (ASFV) entered European Union (EU) in 2014 since then fourteen countries have been affected, Italy North Macedonia being last 2022. While whole genome sequencing remains gold standard for identification new genetic markers, multiple loci with significant variations could be used as rapid cost-effective alternative track outbreaks study evolution...
The role of the ancestral sylvatic cycle African swine fever virus (ASFV) is not well understood in endemic areas eastern Africa. We therefore analysed ASF infection status on samples collected from 51 free-ranging warthogs (Phacocherus africanus) and 1576 Ornithodorus porcinus ticks 26 independent warthog burrows at a single ranch Kenya. Abattoir 83 domestic pigs without clinical symptoms, originating specific locations with no recent reported outbreaks were included this study. All derived...
African swine fever (ASF) is a viral infectious disease of domestic and wild suids all breeds ages, causing wide range hemorrhagic syndromes frequently characterized by high mortality. The endemic in Sub-Saharan Africa Sardinia. Since 2007, it has also been present different countries Eastern Europe, where control measures have not effective so far. continued spread poses serious threat to the industry worldwide. In absence vaccine, early detection infected animals paramount importance for...
African swine fever (ASF) is a complex infectious disease of that constitutes devastating impacts on animal health and the world economy. Here, we investigated evolutionary epidemiology ASF virus (ASFV) in Eurasia Africa using concatenated gene sequences viral protein 72 central variable region isolates collected between 1960 2015. We used Bayesian phylodynamic models to reconstruct history virus, identify population demographics quantify dispersal patterns host species. Results suggest ASFV...
African swine fever (ASF) is currently the major concern of global industry, as a consequence which reconsideration containment and prevention measures taken to date urgently required. A great interest in developing an effective safe vaccine against ASF virus (ASFV) infection has, therefore, recently appeared. The objective present study test inactivated ASFV preparation under vaccination strategy that has not previously been tested order improve its protective effect. following have...
This study aimed to compare the infection dynamics of three genotype II African swine fever viruses (ASFV) circulating in Europe. Eighteen domestic pigs divided into groups were infected intramuscularly or by direct contact with two haemadsorbent ASFVs (HAD) from Poland (Pol16/DP/ OUT21) and Estonia (Est16/WB/Viru8), Latvian non-HAD ASFV (Lv17/WB/Rie1). Parameters, such as symptoms, pathogenicity, distribution virus tissues, humoral immune response, dissemination blood, oropharyngeal rectal...