A5019187834- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- T-cell and Retrovirus Studies
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Viral Infections and Vectors
- Food Waste Reduction and Sustainability
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- Municipal Solid Waste Management
- Sustainable Supply Chain Management
- Tumors and Oncological Cases
- Teratomas and Epidermoid Cysts
- Head and Neck Anomalies
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Oral Health Pathology and Treatment
- Nephrotoxicity and Medicinal Plants
- Political theory and Gramsci
- Immune cells in cancer
- Urinary Tract Infections Management
- Malaria Research and Control
University of Lisbon
2013-2024
Centre for Research in Anthropology
2017-2023
CHU Dijon Bourgogne
2018-2019
HES-SO Genève
2011
Centre Hospitalier Régional Universitaire de Brest
2004-2006
Ipca Laboratories (India)
2005
Instituto Nacional de Investigação Agrária e Veterinária
1994
Plum Island Animal Disease Center
1988-1993
Agricultural Research Service
1989
United States Department of Agriculture
1988
African swine fever virus ASFV/NH/P68 is a naturally occurring, non-haemadsorbing and non-fatal isolate. Longitudinal clinical immunological studies on 31 pigs inoculated oronasally or intramuscularly with this isolate defined two discrete groups of animals: those developing ASF chronic type lesions remaining asymptomatic. Animals had viraemia late after infection, NK activity levels close to that control animals high anti-ASFV specific antibodies together marked hypergammaglobulinaemia...
African swine fever (ASF) is an important disease of pigs and outbreaks ASF have occurred in Europe on multiple occasions. To explore the period for which European soft tick species Ornithodoros erraticus (Acari: Argasidae) able to act as a reservoir virus (ASFV) after infected hosts are removed, we collected specimens from farms provinces Alentejo Algarve Portugal during endemic tested them subsequently using cell culture experimental infection. We show that ticks previously may contain...
Two strains of African swine fever virus (ASFV), the high-virulence Lisboa60 (L60) and low-virulence NH/P68 (NHV), which have previously been used in effective immunization/protection studies, were sequenced. Both isolated Portugal during 11-year period after introduction ASFV to European Continent 1957. The predicted proteins coded by both compared, where differences found these also compared other known virulence. This highlighted several genes with significant alterations that may...
African swine fever (ASF) has persisted in Eastern Europe since 2007, and two endemic zones have been identified the central southern parts of Russian Federation. Moderate- to low-virulent ASF virus isolates are known circulate ASF-affected regions. To improve our knowledge transmission animals recovered from infection, an experimental vivo study was carried out. Four domestic pigs were inoculated with NH/P68 virus, previously characterized develop a chronic form ASF. Two additional...
ABSTRACT African swine fever virus (ASFV) codes for a putative histone-like protein (pA104R) with extensive sequence homology to bacterial proteins that are implicated in genome replication and packaging. Functional characterization of purified recombinant pA104R revealed it binds single-stranded DNA (ssDNA) double-stranded (dsDNA) over wide range temperatures, pH values, salt concentrations an ATP-independent manner, estimated binding site size about 14 16 nucleotides. Using site-directed...
The protective immune response to African swine fever virus (ASFV) includes both cellular and serological components. In this study, the role of antibodies in pathogenicity diagnosis (ASF) was explored. Accordingly, total Ig isotype antibody responses against 12 viral proteins previously demonstrated be main targets immunity were evaluated longitudinally collected sera from pigs infected experimentally with non-pathogenic ASFV/NH/P68 isolate. Strong IgG observed E183L/p54,...
The impact of infection by the low-virulent ASFV/NH/P68 (NHV) and highly virulent ASFV/L60 (L60) isolates on porcine macrophages was assessed through quantification IFNalpha, TNFalpha, IL12p40, TGFbeta ASFV genes real-time PCR at 2, 4 6 h post-infection. Increased TNFalpha IL12p40 expression found in with NHV, which lower than L60. Principal component analysis showed a positive interaction cytokines involved cellular immune mechanisms, namely IFNalpha NHV infection. Quantification ELISA...
Abstract African swine fever virus is the etiological agent of a contagious and fatal acute haemorrhagic viral disease for which there are no vaccines or therapeutic options. The ASFV encodes putative E2 ubiquitin conjugating enzyme (ORF I215L) that shows sequence homology with eukaryotic counterparts. In present study, we showed pI215L acts as an E2-ubiquitin like in large range pH values temperatures, after short incubation times. Further experiments revealed polyubiquitinated instead...
Viral interactions with host nucleus have been thoroughly studied, clarifying molecular mechanisms and providing new antiviral targets. Considering that African swine fever virus (ASFV) intranuclear phase of infection is poorly understood, viral interplay subnuclear domains chromatin architecture were addressed. Nuclear speckles, Cajal bodies, promyelocytic leukaemia nuclear bodies (PML-NBs) evaluated by immunofluorescence microscopy Western blot. Further, efficient PML protein knockdown...
The kinetics of African swine fever virus (ASFV) infection in Ornithodoros erraticus ticks were investigated specimens collected the field at different times following an outbreak disease Portugal 1999 and infected experimentally with a isolated from tick during this outbreak. In field, initial screening for ASFV was carried out by PCR, followed attempts to isolate macrophage cultures. Considering total numbers tested independently developmental stages, DNA detected 42.3, 26.4 22.4% weeks 0,...