A5069038083- Enzyme Structure and Function
- Cancer therapeutics and mechanisms
- Computational Drug Discovery Methods
- Bacterial Genetics and Biotechnology
- Synthesis and biological activity
- Protein Structure and Dynamics
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- RNA modifications and cancer
- vaccines and immunoinformatics approaches
- Lung Cancer Treatments and Mutations
- Epigenetics and DNA Methylation
- Tuberculosis Research and Epidemiology
- Genomics and Phylogenetic Studies
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Multicomponent Synthesis of Heterocycles
- HIV Research and Treatment
- Bioactive Compounds and Antitumor Agents
- Quinazolinone synthesis and applications
- Phytochemistry and Bioactivity Studies
- Biochemical and Structural Characterization
- HIV/AIDS drug development and treatment
Koneru Lakshmaiah Education Foundation
2020-2024
International Institute of Information Technology
2015
Indira Gandhi National Open University
2015
Sanford Burnham Prebys Medical Discovery Institute
2008-2010
Joint Center for Structural Genomics
2008-2010
University of California, San Diego
2010
Discovery Institute
2010
Institute for Medical Research
2009
COVID-19 caused by SARS-CoV-2 was declared a global pandemic WHO (World Health Organization) in March, 2020. Within 6 months, nearly 750,000 deaths are claimed across the globe. This called for immediate social, scientific, technological, public and community interventions. Considering severity of infection associated mortalities, efforts underway to develop preventive measures against SARS-CoV-2. Among target proteins, Spike (S) glycoprotein (a.k.a S Protein) is most studied known trigger...
It is commonly accepted that proteins have evolutionarily conserved 3-dimensional structures, uniquely defined by their amino acid sequence. Here, we question the direct association of structure to sequence comparing multiple models identical proteins. Rapidly growing structural databases contain determined independently times. We collected these in database redundant sets protein structures and then derived conformational states clustering with low root-mean-square deviations (RMSDs). The...
Genomic correlates of evolutionary adaptation to very low or high optimal growth temperature (OGT) values have been the subject many studies. Whereas these provided a protein-structural rationale activity and stability globular proteins/enzymes, point has neglected that extreme temperatures could also resulted from an increased use intrinsically disordered proteins (IDPs), which are resistant conditions in vitro. Contrary expectations, we found conspicuously level structural disorder...
Abstract A novel Coronavirus (SARS-COV-2) has now become a global pandemic. Considering the severity of infection and associated mortalities, there is an urgent need to develop effective preventive measure against this virus. In study, we have designed vaccine construct using computational strategies. Spike (S) glycoprotein major antigenic component that trigger host immune responses. Detailed investigation S protein with various immunoinformatics tools enabled us identify 5 MHC I II B-cell...
The protein SO4266 (gi|24375750) from the bacterium Shewanella oneidensis MR-1 is annotated as a member of Pfam PF02661. This family consists Fic (filamentation induced by cAMP) proteins and their relatives, characterized presence well-conserved HPFXXGNG motif.1 biochemistry has not been extensively exact molecular functions remain unknown. From early studies in Escherichia coli, it believed that cAMP may be involved regulatory mechanism cell division, including folate metabolism synthesis...
ECX21941 represents a very large family (over 600 members) of novel, ocean metagenome-specific proteins identified by clustering the dataset from Global Ocean Sampling expedition. The crystal structure reveals unexpected similarity to Sm/LSm proteins, which are important RNA-binding despite no detectable sequence similarity. protein assembles as homopentamer in solution and when expressed Escherichia coli first pentameric for this although actual oligomeric form vivo is currently not known....
The CC chemokine receptor 5 (CCR5) is the principal entry for HIV1 (macrophage tropic) as observed in early stages of viral replication during attachment to host cell. coreceptor switch one possible mechanisms resistance. Hence, molecules designed bind and inhibit CCR5 or CXCR4 would be effective HIV (human immunodeficiency virus) therapy prevent virus, stop further infection cells, allow time its clearance from body (through immunity intervention). aim this study was design safer...
The mutations concerned with non-small cell lung cancer involving epidermal growth factor receptor of tyrosine kinase family have primarily targeted. EGFR inhibitors binding allosterically to C797S mutant enzyme been developed. Here, database building, library screening performing R-group enumeration and scaffold hopping technique for increasing the affinity compounds carried out. Virtual was performed subjecting HTVS, SP XP docking protocol along its relative free energy calculations....
Proteins with the DUF2063 domain constitute a new Pfam family, PF09836. The crystal structure of member this NGO1945 from Neisseria gonorrhoeae, has been determined and reveals that N-terminal is likely to be DNA-binding domain. In conjunction rest protein, involved in transcriptional regulation, which consistent genomic neighborhood analysis. Of 216 currently known proteins contain domain, most significant sequence homologs (∼40–99% identity) are various Haemophilus species. As these...
Pancreatic lipase is one of the crucial lipolytic enzymes gut that actively facilitates digestion and absorption dietary triglycerides cholesteryl esters. Although it has been deemed as most reliable targets for treatment obesity and/or dyslipidemia, to date, orlistat only known FDA-approved, effective, oral pancreatic inhibitor available clinical use apart from centrally acting antiobesity agents. However, be associated with adverse gastrointestinal renal complications. In this study, we...
BioInt, a biological programming application framework and interpreter, is an attempt to equip the researchers with seamless integration, efficient extraction effortless analysis of data from various databases algorithms. Based on type data, algorithms related functionalities, biology-specific was developed which has nine modules. The modules are compilation numerous reusable BioADTs. This software ecosystem containing more than 450 objects underneath interpreter makes it flexible,...
The crystal structure of Jann_2411 from Jannaschia sp. strain CCS1, a member the Pfam PF07336 family classified as domain unknown function (DUF1470), was solved to resolution 1.45 Å by multiple-wavelength anomalous dispersion (MAD). This protein is first structural representative DUF1470 family. Structural analysis revealed two-domain organization, with N-terminal presenting new fold called ABATE that may bind an yet ligand. C-terminal forms treble-clef zinc finger likely be involved in DNA...
The crystal structures of BB2672 and SPO0826 were determined to resolutions 1.7 2.1 Å by single-wavelength anomalous dispersion multiple-wavelength dispersion, respectively, using the semi-automated high-throughput pipeline Joint Center for Structural Genomics (JCSG) as part NIGMS Protein Structure Initiative (PSI). These proteins are first structural representatives PF06684 (DUF1185) Pfam family. analysis revealed that both adopt a variant Bacillus chorismate mutase fold (BCM). biological...
The switching on or off of methylation, a change from normal methylation to hyper hypo is implicated in many diseases that include cancers, infectious, neurodegenerative and others. Methyltransferases are one the most sought targets have diversified for variety substrates. However, without S-adenosyl-l-methionine (SAM), universal methyl donor, majority methyltransferases remain functionally inactive. In this article, we did comprehensive analysis all available SAM-receptor crystal structures...
Introduction: Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), has its natural history tracing back to 70,000 years. Latent M.tb infection is the reservoir of TB epidemic. becoming more prevalent and acquiring multidrug resistance among first-line antibiotics. Methods: Methylation one main mechanisms through which bacteria develop resistance, hence targeting methyltransferases provides opportunity achieve two-birds-with-one-stone: a) antibiotic: inhibiting translation activity...