A5067016340- Inflammatory Myopathies and Dermatomyositis
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Muscle Physiology and Disorders
- Clusterin in disease pathology
- Nuclear Structure and Function
- Rheumatoid Arthritis Research and Therapies
- Machine Learning in Bioinformatics
- Viral Infections and Immunology Research
- Parkinson's Disease and Spinal Disorders
- Exercise and Physiological Responses
- MicroRNA in disease regulation
- Biomarkers in Disease Mechanisms
- Cancer-related molecular mechanisms research
- Muscle and Compartmental Disorders
- Bone Metabolism and Diseases
- Heat shock proteins research
- Circular RNAs in diseases
- Neurogenetic and Muscular Disorders Research
- Systemic Sclerosis and Related Diseases
- Systemic Lupus Erythematosus Research
- Lipoproteins and Cardiovascular Health
- Multiple Sclerosis Research Studies
- Cytokine Signaling Pathways and Interactions
- Psoriasis: Treatment and Pathogenesis
- Parkinson's Disease Mechanisms and Treatments
Institute of Rheumatology
2016-2025
Charles University
2016-2020
Center for Rheumatology
2016-2018
Biomedical Research Center of the Slovak Academy of Sciences
2016-2017
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2015
Slovak Academy of Sciences
2015
MicroRNAs (miRNAs) are small RNAs that regulate gene expression by targeting mRNA. It was proved some miRNAs significantly deregulated in rheumatoid arthritis (RA). MicroRNA-125b negatively regulates of TNF-α, which plays a crucial role RA pathogenesis. The aim this study to determine the treatment outcome patients with early based on circulating and cellular miR-125b. Total RNA isolated from plasma peripheral blood mononuclear cells (PBMCs) 58 before three months after initiation 54 age-...
Abstract Objectives Objective of this work was to examine myomiR levels in plasma, skeletal muscle, and muscle cells patients with idiopathic inflammatory myopathy (IIM), their interrelations the disease-related clinical phenotypes effects disease-modifying 6-month training-intervention. Methods Samples vastus lateralis (n = 12/13) plasma 20/21) were obtained from IIM healthy controls, respectively. Muscle before after a training-intervention 7 patients. MyomiRs miR-1,-206,-133a,-133b...
Low levels of vitamin D have been associated with several autoimmune diseases. A growing body evidence supports the association skeletal muscle damage, regeneration, and energy lipid metabolism. The aim was to analyse its receptor (VDR) in tissue patients idiopathic inflammatory myopathies (IIM) relate them clinical parameters Forty-six IIM 67 healthy controls (HC) were included study. 27 participated a 24-week exercise intervention. Muscle biopsies obtained from 7 before/after training, 13...
Introduction The aim of the study was to analyse genetic architecture RA by utilizing multiparametric statistical methods such as linear discriminant analysis (LDA) and redundancy (RDA). Methods A total 1393 volunteers, 499 patients with 894 healthy controls were included in study. presence shared epitope (SE) HLA-DRB1 11 SNPs (PTPN22 C/T (rs2476601), STAT4 G/T (rs7574865), CTLA4 A/G (rs3087243), TRAF1/C5 (rs3761847), IRF5 T/C (rs10488631), TNFAIP3 (rs5029937), AFF3 A/T (rs11676922), PADI4...
Abstract Background The structural and functional changes of the skeletal muscles in idiopathic inflammatory myopathies (IIM) caused by inflammation immune can be severely disabling. objective this study was to assess effect a 24-week program combining supervised training activities daily living (ADL), resistance, stability with home exercise for improving muscle function, compared home-based representing regular outpatient care. Methods Fifty-seven patients IIM were consecutively...
The aim of this study was to investigate the systemic and skeletal muscle levels atrophy-associated myokines in patients with idiopathic inflammatory myopathies (IIM) their association clinical characteristics myositis.A total 94 IIM 162 healthy controls were recruited. Of those, 20 28 underwent a biopsy. Circulating concentrations myostatin, follistatin, activin A TGF-β1 assessed by ELISA. expression associated genes involved myostatin signalling pathway tissue determined real-time PCR.We...
Abstract Clusterin (CLU) is a molecular chaperone that participates in variety of biological processes. Recent studies indicate its possible involvement the development bone erosions and autoimmunity. The aim this study was to investigate serum concentrations patients with early rheumatoid arthritis (RA) explore their potential relationship disease activity treatment response. Serum levels CLU were measured 52 before 3 months after initiation healthy individuals. at baseline significantly...
Regular exercise improves muscle functional capacity and clinical state of patients with idiopathic inflammatory myopathy (IIM). In our study, we used an in vitro model human primary cell cultures, derived from IIM before after a 6-month intensive supervised training intervention to assess the impact disease on lipid metabolism dynamics. We provide evidence that cells display altered dynamics impaired adaptive response saturated fatty acid load compared healthy controls. A mitigated effects...
Background Idiopathic inflammatory myopathies (IIM) are chronic disorders characterised, apart from extramuscular manifestations, by symmetrical progressive muscle weakness that may persist even after pharmacological suppression of inflammation, suggesting a significant involvement nonimmune mechanisms. Low levels vitamin D have been associated with several autoimmune diseases. Vitamin is essential for the maintenance skeletal muscle, and mounting evidence supports its relation to damage,...
<h3>Background and objectives</h3> MicroRNAs (miRNAs) are small RNAs that regulate gene expression by targeting mRNA. It was proved some miRNAs significantly deregulated in rheumatoid arthritis (RA). MicroRNA-125b negatively regulates of TNF-α, which plays a crucial role RA pathogenesis. The aim this study to evaluate the miRNA-125b peripheral blood mononuclear cells (PBMCs) its association with disease activity treatment response early patients. <h3>Materials methods</h3> A total 58 (42...
<h3>Background</h3> Idiopathic inflammatory myopathies (IIM) are characterised by inflammation and atrophy of skeletal muscles, pulmonary articular involvement, which limit the mobility/self-sufficiency patients, can have a negative impact on body composition. <h3>Objectives</h3> To assess composition physical activity IIM patients healthy controls (HC). <h3>Methods</h3> 54 with (45 females/9 males; mean age 57.7; disease duration 5.8 years; polymyositis (PM, 22)/dermatomyositis (DM,...
<h3>Background</h3> Idiopathic inflammatory myopathy are characterized by muscle weakness, caused inflammation and immune changes in the affected muscles, which lead to a limitation execution of day-to-day activities (ADL). The aim our study was investigate impact specialized intensive aDL training on strength endurance, depression QoL IIM patients. <h3>Objectives</h3> included total 50 patients who fulfilled Bohan Peter 1975 criteria had skeletal involvement. 27 were recruited into...
Background: Skeletal muscle, pulmonary and articular involvement in idiopathic inflammatory myopathies (IIM) limit the mobility/self-sufficiency of patients, can have a negative impact on body composition. Objectives: The aim was to assess composition physical activity IIM patients healthy controls (HC) association with selected cytokines/chemokines laboratory markers nutrition lipid metabolism. Methods: 54 (45 females; mean age 57.7; disease duration 5.8 years; polymyositis (PM, 22) /...
Idiopathic inflammatory myopathies/IIM are associated with muscle atrophy and metabolic dysfunction. Regular exercise improves function patients’ clinical state. Limited evidence points at low circulating vitamin D/vitD levels in IIM patients, which could contribute to Previously, we showed that altered lipid metabolism cells from patients was normalized by 6-month training. Our aim assess (i) total & active/A (1,25-diOH) vitD serum of before after training intervention healthy controls,...
<h3>Background</h3> Heat shock proteins (Hsps) are molecular chaperones playing a key role in muscle physiology, adaptation to exercise or stress, and activation of inflammatory cells. Increased Hsp90 expression was demonstrated regenerating atrophic fibres, infiltrates. The aim this study evaluate the circulation patients with idiopathic myopathies (IIM) characterise its potential association IIM-related features. <h3>Methods</h3> A total 277 IIM (198 females, 79 males; mean age 54.8;...
<h3>Background and objectives</h3> Interleukin 20 (IL-20), a member of IL-10 cytokine family, is pleiotropic promoting inflammation, angiogenesis, chemotaxis implicated in the pathogenesis rheumatoid arthritis (RA). In this study, serum levels IL-20 were analysed relation to disease activity response therapy active patients with established RA who started biologic rituximab (RTX). <h3>Materials methods</h3> levelswere 27 (24 females) at baseline after 16 weeks treatment; 49 age- sex-matched...
<h3>Background</h3> Heat shock proteins (Hsps) are chaperones playing important roles in skeletal muscle physiology, adaptation to exercise/stress, and activation of inflammatory cells. <h3>Objectives</h3> To assess Hsp90 expression biopsies plasma patients with idiopathic myopathies (IIM) characterize its association IIM-related features. <h3>Methods</h3> Total 277 IIM (198 females, 79 males; mean age 54.8; disease duration 4.1 years; dermatomyositis (DM, 104)/polymyositis (PM, 104)/cancer...
<h3>Background</h3> Heat shock proteins (Hsps) are chaperones playing important roles in skeletal muscle physiology, adaptation to exercise or stress, and activation of inflammatory cells. The aim our study was assess Hsp90 expression biopsies plasma patients with idiopathic myopathies (IIM) characterise its association IIM-related features. <h3>Methods</h3> Total 277 IIM (198 females, 79 males; mean age 54.8; disease duration 4.1 years; dermatomyositis (DM, 104)/polymyositis (PM,...
<h3>Background</h3> Clusterin (apolipoprotein J) is an extracellular chaperone involved in the number of biological processes, such as inflammation and apoptosis. Recent data suggest its protective role autoimmune diseases. The aim this study was to analyse serum levels clusterin expression skeletal muscle specimens patients with idiopathic inflammatory myopathies (IIM) vs healthy controls, investigate association disease activity. <h3>Materials methods</h3> were measured by ELISA...
<h3>Background</h3> Systemic sclerosis (SSc) is characterised by fibrosis of the skin and visceral organs, especially digestive tract, musculoskeletal involvement, which limit mobility/self-sufficiency patients, can have a negative impact on body composition. <h3>Objectives</h3> To assess composition physical activity SSc patients healthy controls (HC). <h3>Methods</h3> 59 with (50 females, 9 males; mean age 52.1; disease duration 6.7 years; limited cutaneous (lcSSc,36)/diffuse (dcSSc,23))...
<h3>Background</h3> Idiopathic inflammatory myopathies (IIM) are characterized by inflammation and atrophy of skeletal muscles, pulmonary articular involvement, which limit the mobility/self-sufficiency patients, can have a negative impact on body composition. <h3>Objectives</h3> To assess composition physical activity IIM patients healthy controls (HC). <h3>Methods</h3> 54 with (45 females/9 males; mean age 57.3; disease duration 5.8 years; polymyositis (PM,22)/dermatomyositis...