A5040096657- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- MicroRNA in disease regulation
- Autoimmune and Inflammatory Disorders Research
- Spondyloarthritis Studies and Treatments
- Systemic Sclerosis and Related Diseases
- Cancer-related molecular mechanisms research
- Bone and Joint Diseases
- Circular RNAs in diseases
- Inflammatory Myopathies and Dermatomyositis
- Cytokine Signaling Pathways and Interactions
- Inflammasome and immune disorders
- Psoriasis: Treatment and Pathogenesis
- Adipokines, Inflammation, and Metabolic Diseases
- Osteoarthritis Treatment and Mechanisms
- Galectins and Cancer Biology
- Autoimmune Bullous Skin Diseases
- Toxin Mechanisms and Immunotoxins
- Extracellular vesicles in disease
- Lymphoma Diagnosis and Treatment
- Musculoskeletal synovial abnormalities and treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Protein Tyrosine Phosphatases
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
Charles University
2015-2024
Institute of Rheumatology
2015-2024
LGBT Foundation
2023
Galicia Sur Biomedical Foundation
2023
Servicio Gallego de Salud
2023
University of Gothenburg
2023
Amsterdam University Medical Centers
2023
University of Amsterdam
2023
Hospital Garcia de Orta
2022
University of Lisbon
2022
<h3>Background</h3> Identification of parameters for early diagnosis and treatment response would be beneficial patients with rheumatoid arthritis (ERA) to prevent ongoing joint damage. miRNAs have features potential biomarkers, an altered expression was shown in established (RA). <h3>Objective</h3> To analyse RA associated the sera ERA find markers disease, clinical activity or predictors disease outcome. <h3>Methods</h3> Total RNA isolated from whole (prior after 3 12 months therapy...
Scientific interest in adipose tissue-derived peptides has increased dramatically recent years (1).Several mediators known as adipo(cyto)kines were first associated with the pathophysiology of obesity-related complications; however a significant role for adipokines such leptin, adiponectin, resistin and visfatin regulating immune responses inflammation recently been discovered (1, 2).Several reports (3-6) have already demonstrated association these severity rheumatoid arthritis (RA).Vaspin,...
<h3>Objective</h3> Fibrin deposits are characteristic of the synovial tissues in rheumatoid arthritis (RA). Once citrullinated, fibrin becomes an autoantigen and is thought to contribute this way perpetuate disease. Our study aimed analyse responses RA fibroblasts (RASF) native citrullinated fibrin. <h3>Methods</h3> The transcriptome induced by RASF was approached with whole-genome-based gene expression arrays. upregulation selected pro-inflammatory genes confirmed additional primary cell...
MicroRNAs (miRNAs) are small RNAs that regulate gene expression by targeting mRNA. It was proved some miRNAs significantly deregulated in rheumatoid arthritis (RA). MicroRNA-125b negatively regulates of TNF-α, which plays a crucial role RA pathogenesis. The aim this study to determine the treatment outcome patients with early based on circulating and cellular miR-125b. Total RNA isolated from plasma peripheral blood mononuclear cells (PBMCs) 58 before three months after initiation 54 age-...
Objectives Dysregulation of miRNAs and their target genes contributes to the pathophysiology autoimmune diseases. Circulating may serve as diagnostic/prognostic biomarkers. We aimed investigate association between circulating miRNAs, disease activity spinal involvement in patients with axial spondyloarthritis (AxSpA). Methods Total RNA was isolated from plasma non-radiographic (nr)AxSpA, ankylosing spondylitis (AS) healthy controls (HC) via phenol-chloroform extraction. A total 760 were...
Abstract Background The presence of ACPA significantly increases the risk developing RA. Dysregulation lymphocyte subpopulations was previously described in Our objective to propose predictive model for progression clinical arthritis based on peripheral subsets and individuals who are at Methods study included 207 at-risk defined by arthralgias either additional positivity or meeting EULAR definition clinically suspect arthralgia. For construction models, 153 with symptom duration ≥12 months...
Objectives. IL-35 is a member of the IL-12 family consisting p35/IL-12a and EBI3/IL-27b subunits. exerts immunomodulatory activities in experimental human autoimmune inflammatory conditions. Our aim was to assess expression skin circulation SSc patients characterize its potential association with SSc-related features.
To investigate the role of protein tyrosine phosphatase nonreceptor type 2 (PTPN2) in pathogenesis rheumatoid arthritis (RA).Synovial tissue samples from patients with RA and osteoarthritis (OA) were stained for PTPN2. Synovial fibroblasts stimulated tumor necrosis factor (TNF) interleukin-1β (IL-1β), lipopolysaccharide (LPS), TRAIL, or thapsigargin. The expression PTPN2 synovial peripheral blood mononuclear cells (PBMCs) was analyzed by real-time polymerase chain reaction Western blotting....
Objective To evaluate circulating visfatin and its relationship with disease activity serum lipids in patients early, treatment-naïve rheumatoid arthritis (RA). Methods Serum was measured 40 early RA before after three months of treatment 30 age- sex-matched healthy individuals. Disease assessed using the Activity Score for 28 joints (DAS28) at baseline 12 months. Multivariate linear regression analysis performed to whether improved is related or a change level. Results significantly...
The purpose of this study was to evaluate and compare the serum levels local expression resistin in patients with idiopathic inflammatory myopathies controls, determine relationship between levels, inflammation disease activity. Serum were determined 42 27 healthy controls. association among inflammation, global activity muscle strength examined. tissues from controls evaluated. Gene protein release resistin-stimulated mononuclear cells assessed. In myopathies, significantly higher than...
Objectives To study the association of systemic and local interleukin-35 (IL-35) levels in rheumatoid arthritis. Methods 37 patients with treatment naïve early RA, 49 established RA 29 control osteoarthritis (OA) were studied. Serum paired synovial fluid samples analysed for IL-35. Disease activity was assessed according to 28-Joint Count Activity Score (DAS28). Results The serum IL-35 significantly higher compared those disease OA subjects. In addition, decreased 12 weeks after initiation...
Polypharmacy (PP), the prescribing of multiple drugs for an individual, is rising in prevalence. PP associates with increased risk adverse drug reactions (ADR) and hospital admissions. We investigated relationship between PP, characteristics rheumatoid arthritis (RA), unplanned admissions.Patients from a RA cohort were retrospectively analyzed. Information was collected electronic medical records. Cox proportional hazards used to compare hospitalization according levels PP. Admissions...
Rheumatoid arthritis (RA) treatment paradigms have shifted over the last two decades. There has been increasing emphasis on combination disease modifying anti-rheumatic drug (DMARD) therapy, newer biologic therapies become available and there is a greater focus achieving remission. We evaluated impact of changes activity scores for 28 joints (DAS28) disability measured by health assessment questionnaire (HAQ). Four cross-sectional surveys between 1996 2014 in adjacent secondary care...