- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant Molecular Biology Research
- Soybean genetics and cultivation
- Phagocytosis and Immune Regulation
- Plant nutrient uptake and metabolism
- Refrigeration and Air Conditioning Technologies
- Immune cells in cancer
- 3D Printing in Biomedical Research
- Turbomachinery Performance and Optimization
- Plant Reproductive Biology
- Phytochemicals and Antioxidant Activities
- Erythrocyte Function and Pathophysiology
- Microbial Metabolic Engineering and Bioproduction
- Lipid metabolism and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Tea Polyphenols and Effects
- Regional Economic and Spatial Analysis
- Light effects on plants
- Blood properties and coagulation
- Belt Conveyor Systems Engineering
- Animal Disease Management and Epidemiology
- Biological Activity of Diterpenoids and Biflavonoids
- Silk-based biomaterials and applications
- Cyclone Separators and Fluid Dynamics
Chinese Academy of Sciences
2018-2024
Northeast Institute of Geography and Agroecology
2020-2024
University of Chinese Academy of Sciences
2022-2024
Shaoyang University
2023
Lanzhou Institute of Chemical Physics
2023
Xinjiang Technical Institute of Physics & Chemistry
2022
University of Science and Technology of China
2009-2022
Hefei Institutes of Physical Science
2018-2022
High Magnetic Field Laboratory
2022
Sichuan Academy of Agricultural Sciences
2022
Photosynthetic microalgae are emerging as potential biomass feedstock for sustainable production of biofuels and value-added bioproducts. CO2 biomitigation through these organisms is considered an eco-friendly promising alternative to the existing carbon sequestration methods. Nonetheless, inherent relatively low photosynthetic capacity has hampered practical use this strategy applications. Here, we demonstrate feasibility improving by genetic manipulation Calvin cycle in typical green...
Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new formation. Recently, as a valuable alternative, bottom-up TE approach utilizing cell-loaded micrometer-scale modular components building blocks reconstruct in vitro or vivo has proved demonstrate number desirable advantages compared with traditional bulk scaffold based top-down...
The green alga Chlorella zofingiensis has been recognized as an industrially relevant strain because of its robust growth under multiple trophic conditions and the potential for simultaneous production triacylglycerol (TAG) high-value keto-carotenoid astaxanthin. Nevertheless, mechanism TAG synthesis remains poorly understood in C. zofingiensis. Diacylglycerol acyltransferase (DGAT) is thought to catalyze committed step assembly Kennedy pathway. genome predicted possess eleven putative...
Abstract Root growth and development depend on continuous cell division differentiation in root tips. In these processes, reactive oxygen species (ROS) play a critical role as signaling molecules. However, few ROS regulators have been identified. this study, we found knockdown of syntaxin gene, SYNTAXIN OF PLANTS81 Arabidopsis thaliana (AtSYP81) resulted severe reduction meristem activity disruption stem niche (SCN) identity. Subsequently, AtSYP81 was highly expressed roots localized the...
Ambient PM2.5 is one of the environmental risk factors and was correlated with senescence-related diseases based on epidemiologic investigation. However, little known about senescence induced by as well underlying mechanisms. In this study, we demonstrated that exposure aggravated cellular in vivo vitro, disrupted micronuclei (MN) played a vital role process. Our results suggested nuclear envelope (NE) PM2.5-induced MN ruptured. Subsequently, cGAS found to localize approximately 80% but few...
Abstract Plant height and flowering time are important agronomic traits that directly affect soybean [Glycine max (L.) Merr.] adaptability yield. Here, the Glycine long internode 1 (Gmlin1) mutant was selected from an ethyl methyl sulfonate (EMS)-mutated Williams 82 population due to its internodes early flowering. Using bulked segregant analysis (BSA), Gmlin1 locus mapped Glyma.02G304700, a homologue of Arabidopsis HY2 gene, which encodes phytochromobilin (PΦB) synthase involved in...
Signal regulatory proteins (SIRPs) comprise a family of cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. Although extracellular domains SIRPs are highly similar, classical motifs cytoplasmic or transmembrane distinguish them as either activating (β) inhibitory (α) isoforms. We reported previously that human neutrophils (polymorphonuclear (PMN)) express multiple SIRP isoforms SIRPα binding to its ligand CD47 regulates PMN transmigration....
Abstract MutL homolog 1 (MLH1), a member of the family, is required for normal recombination in most organisms. However, its role soybean (Glycine max) remains unclear to date. Here, we characterized Glycine max female and male sterility (Gmfms1) mutation that reduces pollen grain viability increases embryo sac abortion soybean. Map-based cloning revealed causal gene Gmfms1 (GmMLH1), CRISPR/Cas9 knockout approach further validated disruption GmMLH1 confers female–male phenotype Loss function...
Abstract Signal regulatory proteins (SIRP-α, -β, and -γ) are important regulators of several innate immune functions that include leukocyte migration. Membrane distal (D1) domains SIRPα SIRPγ, but not SIRPβ, mediate binding to a cellular ligand termed CD47. Because the extracellular all SIRPs highly homologous, we hypothesized some 16 residues unique SIRPα.D1 By site-directed mutagenesis, determined CD47 is independent N-glycosylation. We also identified three critical for by exchanging on...
Long-chain acyl-coenzyme A (CoA) synthetase (LACS) catalyzes the formation of acyl-CoAs from free fatty acids, which is pivotal for lipid metabolism. Here, we confirmed presence six CzLACS genes in Chromochloris zofingiensis. Functional complementation and vitro enzymatic assay indicated that CzLACS2 through CzLACS5 rather than CzLACS1 or CzLACS6 are bona fide LACS enzymes they have overlapping yet distinct substrate preference. The results subcellular colocalization experiment different...
The plant cytoskeleton forms a stereoscopic network that regulates cell morphogenesis. also provides tracks for trafficking of vesicles to the target membrane. Fusion with membrane is promoted by SNARE proteins, etc. vesicle-SNARE, Sec22, between ER and Golgi in yeast mammals. Arabidopsis AtSEC22 might regulate early secretion essential gametophyte development. However, role development unclear. To clarify regulation development, we isolated an knock-down mutant, atsec22-4 , found...
Leisure dried tofu (LD-tofu) was prepared using two different marinating processes: the repeated heating method (RHM) and vacuum pulse (VPM). The quality characteristics bacterial community succession of LD-tofu marinade were evaluated. results showed that nutrients in easily dissolved into during process, while protein moisture content RHM changed most dramatically. With increase recycling times, springiness, chewiness hardness VPM increased significantly. total viable count (TVC) decreased...
Map-based cloning revealed that two novel soybean distorted trichome mutants were due to loss function of GmNAP1 gene, which affected the morphology and pavement cell ploidy by regulating actin filament assembly. Trichomes increase both biotic abiotic stress resistance in soybean. In this study, Gmdtm1-1 Gmdtm1-2 with shorter trichomes bigger epidermal cells isolated from an ethyl methylsulfonate mutagenized population. Both them had reduced plant height smaller seeds. bulked segregant...
The aim of the study was to investigate anti-tumoral activity morelloflavone substances with different structures. We also studied possible link between structure and its function. Various types chromatographic techniques were used isolate screen from extracts gambogic tree trunk structures identified by analytical such as high resolution mass spectrometry nuclear magnetism. Anti-tumoral activities compounds investigated antitumor compound exaimed. Our results showed that isolated...
This A novel high frequency voltage (HV) generator with silicon carbide (SiC) power semiconductor devices is proposed in this paper to achieve energy efficiency, fast HV pulse speed and compact size advantages. The electrical insulation design analysis transformer multiplier circuit based on 1.2kV SiC Schottky diode are discussed details. MOSFETs introduced the inverter switching from 300 kHz 500 kHz. characteristics detailed consideration gate driver given. packaging considerations for...
A simple, label-free colorimetric method for the determination of fibrinogen (Fib) in plasma is presented.
The generation of oxygen and organic matter in plants mainly depends on photosynthesis, which directly affects plant growth development. chloroplast is the main organelle photosynthesis occurs. In this study, a Glycine max pale green leaf 3-1 ( Gmpgl3-1 ) mutant was isolated from soybean mutagenized population. presented with decreased chlorophyll contents, reduced stroma thylakoids, yields, numbers pods per plant. Bulked segregant analysis (BSA) together map-based cloning revealed...