A5100458400- Medical Imaging Techniques and Applications
- Digital Radiography and Breast Imaging
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Advanced X-ray and CT Imaging
- Neonatal Respiratory Health Research
- RNA and protein synthesis mechanisms
- interferon and immune responses
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Medical Imaging and Pathology Studies
- DNA and Nucleic Acid Chemistry
- Cancer-related molecular mechanisms research
- Cytokine Signaling Pathways and Interactions
- Ferroptosis and cancer prognosis
- Aquaculture disease management and microbiota
- RNA Research and Splicing
- Telomeres, Telomerase, and Senescence
- Renal and related cancers
- Graphene and Nanomaterials Applications
- Infrared Thermography in Medicine
- Influenza Virus Research Studies
- Genomics and Chromatin Dynamics
- Cardiac Imaging and Diagnostics
- Lung Cancer Diagnosis and Treatment
Anhui University
2023-2024
Ningbo Medical Center Lihuili Hospital
2023-2024
Ningbo University
2023-2024
Institute of Microbiology
2017-2024
Chinese Academy of Sciences
2015-2024
State Key Laboratory of Chemobiosensing and Chemometrics
2020-2024
Hunan University
2020-2024
Fujian Agriculture and Forestry University
2024
Xuzhou Medical College
2024
Wuhan University
2008-2023
Abstract Corynebacterium glutamicum is an important industrial metabolite producer that difficult to genetically engineer. Although the Streptococcus pyogenes ( Sp ) CRISPR-Cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into C. . Here we report a Francisella novicida Fn CRISPR-Cpf1-based genome-editing method CRISPR-Cpf1, combined with single-stranded DNA (ssDNA) recombineering, precisely introduces small changes bacterial at efficiencies...
We describe a facile approach to controllable assembly of monodisperse Fe3O4 nanoparticles (NPs) on chemically reduced graphene oxide (rGO). First, reduction and functionalization GO by polyetheylenimine (PEI) were achieved simultaneously simply heating the PEI mixture at 60 °C for 12 h. The process is environmentally friendly convenient compared with previously reported methods. Meso-2,3-dimercaptosuccinnic acid (DMSA)-modified NPs then conjugated moiety which located periphery sheets via...
X‐ray projection mammography, using a film/screen combination, or digital techniques, has proven to be the most effective imaging modality currently available for early detection of breast cancer. However, inherent superimposition structures makes small carcinoma (a few millimeters in size) difficult detect when it is occult dense breasts, leading high false‐positive biopsy rate. Cone‐beam x‐ray‐projection‐based volume flat panel detectors (FPDs) may allow obtaining three‐dimensional images,...
Th2-high asthma is characterized by elevated levels of type 2 cytokines, such as interleukin 13 (IL-13), and its prevalence has been increasing worldwide. Ferroptosis, a recently discovered programmed cell death, involved in the pathological process asthma; however, underlying mechanisms remain incompletely understood. In this study, we demonstrated that serum level malondialdehyde (MDA), an index lipid peroxidation, positively correlated with IL-13 negatively predicted forced expiratory...
Preliminary evaluation of recently developed large-area flat panel detectors (FPDs) indicates that FPDs have some potential advantages: compactness, absence geometric distortion and veiling glare with the benefits high resolution, detective quantum efficiency (DQE), frame rate dynamic range, small image lag (<1%), excellent linearity (/spl sim/1%). The advantages new FPD make it a promising candidate for cone-beam volume computed tomography (CT) angiography (CBVCTA) imaging. purpose this...
Genetic modifications of bacterial chromosomes are important for both fundamental and applied research. In this study, we developed an efficient, easy-to-use system genetic modification the Escherichia coli chromosome, a two-plasmid method involving lambda Red (λ-Red) recombination I-SceI cleavage. An intermediate strain is generated by integration resistance marker gene(s) recognition sites in or near target gene locus, using λ-Red PCR targeting. The transformed with donor plasmid carrying...
Autophagy-related protein 7 (ATG7) is an essential autophagy effector enzyme. Although it well known that plays crucial roles in the infections with various viruses including influenza A virus (IAV), function and underlying mechanism of ATG7 infection pathogenesis IAV remain poorly understood. Here, vitro studies showed had profound effects on replication IAV. Depletion markedly attenuated IAV, whereas overexpression facilitated viral replication. conditional knockout mice were further...
Functionalized multiwalled carbon nanotubes (f-MWNTs) are of great interest and designed as a novel gene delivery system. In this paper, we presented synthesis polyamidoamine-functionalized (PAA-g-MWNTs) their application The PAA-g-MWNTs, obtained from amide formation between PAA chemically oxidized MWNTs, were stable in aqueous solution much less toxic to cells than PEI 25KDa. More importantly, PAA-g-MWNTs showed comparable or even higher transfection efficiency at optimal w/w ratio....
Long noncoding RNAs (lncRNAs) are involved in numerous cellular processes. Increasing evidence suggests that some lncRNAs function immunity through various complex mechanisms. However, implication of a large fraction antiviral innate remains uncharacterized. Here, we identified an lncRNA called IFITM4P was transcribed from interferon-induced transmembrane protein 4 pseudogene (IFITM4P), belonging to the (IFITM) family. We found expression significantly induced by infection with several...
Long noncoding RNAs (lncRNAs) are involved in a diversity of biological processes. It is known that differential expression thousands lncRNAs occurs host during influenza A virus (IAV) infection. However, only few them have been well characterized. Here, we identified lncRNA, named as interferon (IFN)-stimulated lncRNA (ISR), which can be significantly upregulated response to IAV infection mouse model. sequence alignment revealed ISR present mice and human beings, indeed, found it was...
Alveolar formation requires coordinated movement and interaction between alveolar epithelial cells, mesenchymal myofibroblasts, endothelial cells/pericytes to produce secondary septa. These processes rely on the acquisition of distinct cellular properties enable ligand secretion for cell-cell signaling initiate morphogenesis through contraction, cell migration, shape change. In this study, we showed that mitochondrial activity distribution play a key role in bestowing functions both cells...
Circular RNAs (circRNAs) are an important subclass of noncoding implicated in the regulation multiple biological processes. However, functional involvement circRNAs pathogenesis influenza A viruses (IAVs) remains largely unknown. Here, we employed RNA sequencing (RNA-Seq) to examine differentially expressed mouse lung tissues challenged or not with IAV evaluate impact viral infection on
Herein, we subtly engineered a pH and membrane receptor dual-activatable aptamer therapeutic for bispecific tumor cell imaging in situ drug release by utilizing hairpin-contained i-motif as the acid-responsive element to be complementary with tumor-targeted aptamer, named an "molecule-doctor" (pH-Apt-MD). Specifically, pH-Apt-MD consisted of two DNA strands, where Apt-sgc8c was labeled AF488 Cy3 at its 5′- 3′-end, respectively. The I-strand, i-motif, strand partially, BHQ2 middle, thus...
Ambient PM2.5 is one of the environmental risk factors and was correlated with senescence-related diseases based on epidemiologic investigation. However, little known about senescence induced by as well underlying mechanisms. In this study, we demonstrated that exposure aggravated cellular in vivo vitro, disrupted micronuclei (MN) played a vital role process. Our results suggested nuclear envelope (NE) PM2.5-induced MN ruptured. Subsequently, cGAS found to localize approximately 80% but few...
Adequate retention in blood circulation is a prerequisite for construction of gene delivery carriers systemic applications. The stability the bloodstream requires them to effectively resist protein adsorption and maintain small size avoiding dissociation, aggregation, nuclease digestion under salty proteinous medium. Herein, mixture two block catiomers consisting same cationic block, poly{N-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} (PAsp(DET)), but varying shell-forming blocks,...
Pichia pastoris is a common host organism for heterologous protein expression and metabolic engineering. Zeocin-, G418-, nourseothricin- blasticidin-resistance genes are the only dominant selectable markers currently available selecting P. transformants. We describe here new vectors that confer hygromycin resistance base on Klebsiella pneumoniae hph gene. To demonstrate application of intracellular secreted expression, green fluorescent (GFP) human serum albumin (HSA) were cloned into...
Spleen tyrosine kinase (Syk) has recently come forth as a critical regulator of innate immune response. Previous studies identify Syk key for STAT1 activation at the early stage influenza A virus (IAV) infection that is involved in initial antiviral immunity. However, involvement host immunity during late phase IAV and its effect on pathogenesis remain unknown. Here, we found through time course restrained response infection, thereby promoting viral replication. Depletion suppressed...
Accurate and rapid imaging of tumor cells is vital importance for early cancer diagnosis intervention. Aptamer-based fluorescence sensors have become a potent instrument bioimaging, while false positives on-target off-tumors linked to single-biomarker aptasensors compromise the specificity sensitivity imaging. In this paper, we describe sequential response aptasensor precise cell identification that based on DNA "AND" logic gate. Specifically, sensor consists three single-stranded DNA,...
Classical swine fever virus (CSFV) commonly infects the lymphatic tissues and immune cells of pigs could cause a lethal disease in animals. The process release cytokines like type III interferons (IFNs) is one important responses host innate immunity to viral infection. However, little information available about IFN response CSFV In this study, we investigated expression IFNs including interleukin-28B (IL-28B) IL-29 PK-15 following We found that infection with was able induce IL-28B cells,...