A5072588854- Polyamine Metabolism and Applications
- Estrogen and related hormone effects
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Pediatric Hepatobiliary Diseases and Treatments
- Bladder and Urothelial Cancer Treatments
- Gallbladder and Bile Duct Disorders
- Colorectal Cancer Treatments and Studies
- Amino Acid Enzymes and Metabolism
- Genetic factors in colorectal cancer
- Breast Lesions and Carcinomas
- Gastroesophageal reflux and treatments
- Biopolymer Synthesis and Applications
- PI3K/AKT/mTOR signaling in cancer
- Soft tissue tumor case studies
- Sarcoma Diagnosis and Treatment
- Liver Disease Diagnosis and Treatment
- Growth Hormone and Insulin-like Growth Factors
- Prostate Cancer Treatment and Research
- Effects and risks of endocrine disrupting chemicals
- Breast Cancer Treatment Studies
- Digestive system and related health
- Liver physiology and pathology
- Bone health and treatments
- Cancer-related molecular mechanisms research
University of Bari Aldo Moro
2020
Gastroenterology Hospital "Saverio de Bellis"
2009-2018
Tel Aviv Sourasky Medical Center
2015
Laboratoire de Biochimie
1999-2004
Sapienza University of Rome
2004
Istituti di Ricovero e Cura a Carattere Scientifico
1987-2001
University of Turin
1993-1994
Ospedale San Giovanni Bosco
1994
Ospedale di Civita Castellana
1990
Thrombocytopenia has been reported to be associated with small size HCCs, and thrombocytosis large HCCs. The aim was examine the effects of platelets in relation HCC cell growth.The time-expired pooled normal human were examined on line growth invasion.Blood platelet numbers increased increasing tumor portal vein invasion. Platelet extracts enhanced 4 lines, as well migration, medium AFP levels decreased apoptosis. Cell invasion significantly enhanced, using a Matrigel-coated trans-well...
To determine behavioural, dietary and other common factors associated with new cases of gallstones, diagnosed by ultrasonography, in a prospective cohort study conducted southern Italy.Between May 1985 June 1986, systematic sampling from the electoral register Castellana, small town Italy, yielded 2472 subjects who had their gallbladder checked for gallstones ultrasonography. Between 1992 1993, 1962 out 2235 (87.7%) without at baseline agreed to further ultrasound examination. At first...
To evaluate the growth-inhibitory properties of potent multi-kinase antagonist Regorafenib (Fluoro-Sorafenib), which was synthesized as a more Sorafenib, Raf inhibitor and to determine whether similar mechanisms were involved, human hepatoma cell lines grown in presence or absence Regorafanib examined for growth inhibition. Western blots performed targets, apoptosis, autophagy. inhibited Hep3B, PLC/PRF/5, HepG2 cells concentration- time-dependent manner. Multiple signaling pathways altered,...
Chlorogenic acid (CGA) is a polyphenol present in many human dietary foods. Several studies indicated beneficial role of CGA the prevention cancer and an enhancement chemotherapy when combined with treatment hepatocarcinoma (HCC). Drug toxicity, resistance subsequent disease progression represent problem HCC management, although multikinase inhibitor Regorafenib improved overall survival. This study focused on evaluation effects using both low concentrations as natural compound cells. The...
Previous in vitro and vivo studies have suggested that lactobacilli can exert antiproliferative effects on the gastrointestinal epithelium. However, their role affecting cellular proliferative mechanisms is not completely clear. The aim of this study was to investigate increasing concentrations Lactobacillus rhamnosus strain GG (L. GG) homogenate cell growth proliferation (by MTT, [3H]-thymidine incorporation polyamine biosynthesis) neoplasms originating from different tracts. Thus, HGC-27...
Abstract Sorafenib was shown in clinical trial to enhance survival hepatocellular carcinoma (HCC) patients, but with minimal tumor shrinkage. To correlate several indices of HCC growth at various drug concentrations, cells were grown low concentrations two multikinase inhibitors, Regorafenib (Stivarga) and (Nexavar) their effects examined on alpha‐fetoprotein (AFP), cell growth, migration, invasion. In AFP positive human lines, inhibited 0.1–1 µM concentrations. Cell migration invasion also...
Abstract Previous in vivo studies have suggested that lactobacilli can exert anti-proliferative effects on the gastric epithelium. However, few data are available their mechanisms of action. The aim this study was to investigate increasing concentrations Lactobacillus rhamnosus strain GG (L. GG) homogenate cell growth and proliferation [by 3-(4,5 di-methylthiazol-2-yl)–2,5-diphenyltetrazolium bromide, [3H]-thymidine incorporation polyamine biosynthesis] apoptosis processes (by Bax/Bcl-2 mRNA...
Platelets are frequently altered in hepatocellular carcinoma (HCC) patients. Platelet lysates (hPL) can enhance HCC cell growth and decrease apoptosis. The aims were to evaluate whether hPL modulate the actions of Sorafenib or Regorafenib, two clinical multikinase antagonists. Several human lines grown presence absence with without hPL. Growth was measured by MTT assay, apoptosis assessed Annexin V western blot, autophagy MAPK signaling also migration invasion standard vitro assays. Both...
Environmental chemicals, such as heavy metals, affect female reproductive function. A biological sensor of the signals many toxic chemical compounds seems to be aryl hydrocarbon receptor (AHR). Previous studies demonstrated environmental metals in Taranto city (Italy), an area that has been influenced by anthropogenic factors industrial activities and waste treatments since 1986. However, impact these elements on fertility this geographic never analyzed. Thus, present study, we evaluated AHR...
<i>Objective:</i> Farnesyl diphosphate synthase (FPPs) produces FPP which is considered a branch-point intermediate in the synthesis of sterols and isoprenylated cellular metabolites. In this study we investigated whether detectable FPPs activity was present human colorectal cancer (CRC), also evaluating vitro role enzyme growth apoptosis CRC cells by using Pamidronate (PAM), inhibitor. <i>Methods:</i> The level determined normal surrounding mucosa 50 patients...
Chemoprevention by dietary constituents has recently emerged as a novel approach to control gastric cancer incidence. Over the past years, functional foods and food supplements, especially probiotics, have received much attention potential prevention agents. The precise mechanisms which these lactic cultures exert their antitumorigenic activities are not fully elucidated, but there is some evidence of influence on cell proliferation growth. Ornithine decarboxylase (ODC) spermidine/spermine...
// Maria Grazia Refolo 1, * , Rosalba D'Alessandro Catia Lippolis 1 Nicola Carella Aldo Cavallini Caterina Messa and Brian Irving Carr 2 Laboratory of Cellular Molecular Biology, Department Clinical Pathology, National Institute Gastroenterology, "S. De Bellis" Research Hospital, Castellana Grotte, BA, Italy Visiting Professor, Program for Targeted Experimental Therapeutics, Izmir Biomedicine Genome Center, Dokuz Eylul University, Izmir, Turkey These authors have contributed equally to this...
Sorafenib is an FDA-approved agent for treatment of human hepatocellular carcinoma (HCC), but tumor shrinkage minor. We therefore developed a strategy to combine K vitamins with sorafenib treat HCC, and found that this combination enhanced sorafenib-induced HCC cell growth inhibition. To explore the mechanisms involved, we examined role Raf kinase, since both were reported inhibit via signaling pathway. whereas lower concentration vitamin K1 (25 μM) or (2.5 alone slightly induced c-Raf...
Blood platelet numbers are correlated with growth and aggressiveness of several tumor types, including hepatocellular carcinoma (HCC). We previously found that lysates (hPLs) both stimulated HCC cell migration, antagonized the growth-inhibitory apoptotic effects Regorafenib, multikinase inhibitor, on lines. evaluated human insulin-like factor-1 (IGF1), a mitogen contained in platelets, Regorafenib-mediated inhibition.An Elisa kit was used to evaluate hPL IGF1 concentrations. The...
Background Hepatitis C virus (HCV) is a common cause of chronic liver diseases but the degree to which these contribute liver-related mortality not well established. The aim this study was estimate absolute and relative effects HCV infection on mortality.