A5004534716- Mast cells and histamine
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Hepatitis C virus research
- Platelet Disorders and Treatments
- Geology and Paleoclimatology Research
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Cytokine Signaling Pathways and Interactions
- Paleontology and Stratigraphy of Fossils
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Evolution and Paleontology Studies
- Protein Kinase Regulation and GTPase Signaling
- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- interferon and immune responses
- Glycosylation and Glycoproteins Research
- PI3K/AKT/mTOR signaling in cancer
- Plant Pathogens and Fungal Diseases
- Hepatitis B Virus Studies
- Ubiquitin and proteasome pathways
- Click Chemistry and Applications
- Plant and Fungal Species Descriptions
University of Fukui
2015-2024
Adam Mickiewicz University in Poznań
2023
Niigata Prefectural Shibata Hospital
2017
Kobe University
1997-2009
National Institute of Dental and Craniofacial Research
2000-2002
National Institutes of Health
2000-2002
Pearl River Community College
1995
National Cerebral and Cardiovascular Center
1992
Hiroshima University
1985
To explore the mechanism(s) by which Syk protein tyrosine kinase participates in B cell antigen receptor (BCR) signaling, we have studied function of various mutants cells made deficient homologous recombination knockout. Both SH2 domains were required for BCR-mediated and phospholipase C (PLC)-gamma 2 phosphorylation, inositol 1,4,5-triphosphate release, Ca2+ mobilization. A possible explanation this requirement was provided findings that recruitment to tyrosine-phosphorylated...
Non-structural protein 4A (NS4A) of Hepatitis C virus (HCV) functions as a cofactor for NS3 by forming complex with it to augment its enzymic activities. NS4A also forms other HCV proteins, such NS4B/NS5A, facilitate the formation viral RNA replication on endoplasmic reticulum (ER) membrane. In addition essential role in replication, is thought be involved pathogenesis affecting cellular functions. this study, was demonstrated that localized not only ER, but mitochondria when expressed...
The N-terminal 198 residues of NS3 (NS3-N) Hepatitis C virus (HCV) subtype 1b obtained from 29 patients, as well full-length (NS3-Full), were analysed for their subcellular localization, interaction with the tumour suppressor p53 and serine protease activity in presence absence viral cofactor NS4A. Based on subcellular-localization patterns NS4A, NS3-N sequences classified into three groups, each group exhibiting either dot-like, diffuse or a mixed type localization. Chimeric NS3-Full...
Sendai virus (SeV) C protein is a multifunctional that plays important roles in regulating viral genome replication and transcription, antagonizing the host interferon system, suppressing virus-induced apoptosis, facilitating assembly budding. We here report novel role of SeV protein, limitation double-stranded RNA (dsRNA) generation for maintaining rate synthesis infected cells. It was found intracellular maintained even after wild-type (wt) infection, but markedly suppressed following...
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumor factor family that selectively induces apoptosis in cancer cells. However, gastric cells are insensitive to TRAIL. In present study, we show oxaliplatin enhanced TRAIL-induced MGC803, BGC823, and SGC7901 Oxaliplatin promoted death receptor 4 (DR4) 5 (DR5) clustering into aggregated lipid rafts, while cholesterol-sequestering agent nystatin partially prevented raft aggregation, DR4 DR5 clustering, reduced...
To understand in detail the transcriptional and functional overlap of IFN-I- IFN-II-activated responses, we used an integrative RNAseq-ChIPseq approach Huh7.5 cells characterized genome-wide role pSTAT1, pSTAT2, IRF9 IRF1 time-dependent ISG expression. For first time, our results provide detailed insight timely steps IFNα- IFNγ-induced transcription, which pSTAT1- pSTAT2-containing ISGF3 GAF-like complexes are recruited to individual or combined ISRE GAS composite sites a phosphorylation-...
Dectin-1 recognizes β-glucan and plays important roles for the antifungal immunity through activation of spleen tyrosine kinase (Syk) in dendritic cells or macrophages. Recently, expression was also identified human mouse mast cells, although its physiological were largely unknown. In this report, rat cell line RBL-2H3 analyzed to investigate molecular mechanism Dectin-1-mediated responses cells. Treatment with Dectin-1-specific agonist curdlan induced phosphorylation cellular proteins...
Interferon-α (IFN-α) and IFN-λ are structurally distinct cytokines that bind to different receptors, but induce expression of similar sets genes through Janus kinase (JAK)-signal transducers activators transcription (STAT) pathways. The difference between IFN-α signaling remains poorly understood. Here, using the CRISPR/Cas9 system, we examine role STAT1 STAT2 in inhibition hepatitis C virus (HCV) replication by IFN-λ. Treatment with increases IFN-stimulated (ISGs) such as double-stranded...
Abstract P‐glycoprotein (P‐gp)‐mediated multi‐drug resistance (MDR) is a major barrier to the effective chemotherapy of many cancers. Recent studies have shown that inhibition PI3K/Akt signalling pathway can reverse P‐gp‐mediated MDR. We investigated expression activated Akt (p‐Akt) in 124 human gastric carcinoma tissue samples. Ubiquitous p‐Akt was recorded majority (88/124). There significant correlation between and P‐gp. In adriamycin‐resistant MDR cell line SGC7901/ADR, increased...
The protein tyrosine kinase Syk plays an essential role in Fc epsilon RI-mediated histamine release mast cells by regulating the phosphorylation of other proteins. We investigated functional a putative site, Tyr317. This linker region is possible site for binding negative regulator Cbl. with Tyr317 mutated to Phe (Y317F) was expressed Syk-negative variant RBL-2H3 cells. Compared expressing wild-type Syk, expression Y317F mutant resulted increase phospholipase C-gamma and dramatic enhancement...
Abstract Background: Recent studies have demonstrated that c‐Cbl functions as a ubiquitin‐protein ligase toward immune receptors and non‐receptor protein‐tyrosine kinase Syk by facilitating their ubiquitination subsequent targeting to proteasomes. However, it was not clear whether Src family Lyn is regulated the Cbl of ligases. Results: Aggregation high affinity IgE receptor (FcɛRI) induces rapid in rat basophilic leukaemia RBL‐2H3 cells. Treatment cells with proteasome inhibitor enhances...
Adaptor protein 3BP2 positively regulates the high affinity IgE receptor (FcɛRI)‐mediated activation of degranulation in mast cells. Genetic study identified point mutations gene human‐inherited disease cherubism. The multiple cysts cherubism lesion jaw bones are filled with activated osteoclasts and stromal cells, including By over‐expression using rat basophilic leukaemia RBL‐2H3 we have analysed effect on function protein, which plays a positive regulatory role FcɛRI‐mediated cell...
Adaptor protein c-Abl SH3 domain-binding protein-2 (3BP2, also referred to SH3BP2) regulates immune receptor-mediated signal transduction. In this report we focused on the molecular mechanism of 3BP2 function in B cell receptor (BCR) signaling. Engagement BCR induces tyrosine phosphorylation 3BP2. Genetic analysis demonstrated that Syk is critical for BCR-mediated Mutational revealed both Tyr(183) and Src homology 2 (SH2) domain are necessary 3BP2-mediated BCR-induced activation nuclear...
Persistent infection with hepatitis C virus (HCV) is closely correlated type 2 diabetes. In this study, replication of HCV at different glucose concentrations was investigated by using J6/JFH1-derived cell-adapted in Huh-7.5 cells and the mechanism regulation AMP-activated protein kinase (AMPK) as an energy sensor cell analyzed. Reducing concentration culture medium from 4.5 to 1.0 g/L resulted suppression replication, along activation AMPK. Whereas treatment AMPK activator...
Thrombin stimulation induces a dramatic increase in the activity of p72syk platelets. We have found that activated p72syk, which is phosphorylated on tyrosine residue(s), translocates from Triton X-100-soluble fraction to X-100-insoluble, cytoskeleton-rich after thrombin stimulation. In addition, redistribution 100,000 x g X-soluble and membrane skeleton was correlate with an increased level cytoskeleton. Furthermore, early phase translocation (within 60 s) significantly inhibited...
Adaptor protein 3BP2, a c-Abl-Src homology 3 (SH3) domain-binding protein, is known to play regulatory role in T-cell receptor-mediated transcriptional activation of nuclear factor activated T cell and activator 1 by interacting with Syk/ZAP-70 protein-tyrosine kinase. We have previously demonstrated that aggregation high affinity IgE receptor (FcepsilonRI) induces tyrosine phosphorylation overexpression the 3BP2-SH2 domain suppresses antigen-induced degranulation rat basophilic leukemia...