A5046387503- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Endometriosis Research and Treatment
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Hepatitis Viruses Studies and Epidemiology
- Reproductive System and Pregnancy
- Biochemical and Molecular Research
- Viral Infections and Immunology Research
- Endometrial and Cervical Cancer Treatments
- Autophagy in Disease and Therapy
- Uterine Myomas and Treatments
- Systemic Lupus Erythematosus Research
- Health disparities and outcomes
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Diet and metabolism studies
- Chronic Lymphocytic Leukemia Research
- Advanced Thermodynamic Systems and Engines
- Folate and B Vitamins Research
- Chronic Disease Management Strategies
- Chemokine receptors and signaling
Kobe University
2015-2024
Zhejiang University
2004-2024
Hangzhou First People's Hospital
2023-2024
Kobe Institute Of Computing
2024
XinHua Hospital
2023
Shanghai Jiao Tong University
2023
Southeast University
2016-2023
Huazhong University of Science and Technology
2019-2022
Central Hospital of Wuhan
2019-2022
University Hospitals of Cleveland
2021
ABSTRACT We previously reported that cells harboring the hepatitis C virus (HCV) RNA replicon as well those expressing HCV NS3/4A exhibited increased sensitivity to suboptimal doses of apoptotic stimuli undergo mitochondrion-mediated apoptosis (Y. Nomura-Takigawa, et al., J. Gen. Virol. 87:1935-1945, 2006). Little is known, however, about whether or not infection induces virus-infected cells. In this study, by using chimeric J6/JFH1 strain genotype 2a, we demonstrated induced cell death in...
Development of complementary and/or alternative drugs for treatment hepatitis C virus (HCV) infection is still much needed from clinical and economic points view. Antiviral substances obtained medicinal plants are potentially good targets to study. Glycyrrhiza uralensis G. glabra have been commonly used in both traditional modern medicine. In this study, extracts roots their components were examined anti-HCV activity using an HCV cell culture system. It was found that a methanol extract its...
Non-structural protein 4A (NS4A) of Hepatitis C virus (HCV) functions as a cofactor for NS3 by forming complex with it to augment its enzymic activities. NS4A also forms other HCV proteins, such NS4B/NS5A, facilitate the formation viral RNA replication on endoplasmic reticulum (ER) membrane. In addition essential role in replication, is thought be involved pathogenesis affecting cellular functions. this study, was demonstrated that localized not only ER, but mitochondria when expressed...
ABSTRACT Chronic hepatitis C virus (HCV) infection is often associated with type 2 diabetes. However, the precise mechanism underlying this association still unclear. Here, using Huh-7.5 cells either harboring HCV-1b RNA replicons or infected HCV-2a, we showed that HCV transcriptionally upregulated genes for phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase), rate-limiting enzymes hepatic gluconeogenesis. In way, enhanced cellular production of 6-phosphate (G6P)...
The N-terminal 198 residues of NS3 (NS3-N) Hepatitis C virus (HCV) subtype 1b obtained from 29 patients, as well full-length (NS3-Full), were analysed for their subcellular localization, interaction with the tumour suppressor p53 and serine protease activity in presence absence viral cofactor NS4A. Based on subcellular-localization patterns NS4A, NS3-N sequences classified into three groups, each group exhibiting either dot-like, diffuse or a mixed type localization. Chimeric NS3-Full...
Abstract Background Hepatitis C virus (HCV) is a major cause of liver disease and potential substantial morbidity mortality worldwide. The overall prevalence HCV infection 2%, representing 120 million people Current standard treatment using pegylated interferon ribavirin effective in only 50% the patients infected with genotype 1, associated significant side effects. Therefore, it still importance to develop new drugs for HCV. Antiviral substances obtained from natural products, including...
The development of complementary and/or alternative drugs for treatment hepatitis C virus (HCV) infection is still needed. Antiviral compounds in medicinal plants are potentially good targets to study. Morinda citrifolia a common plant distributed widely Indo-Pacific region; its fruits and leaves food sources also used as traditional medicine. In this study, using HCV cell culture system, it was demonstrated that methanol extract, n-hexane, ethyl acetate fractions from M. possess anti-HCV...
Abstract Hepatitis C virus (HCV), dengue (DENV) and Japanese encephalitis (JEV) belong to the family Flaviviridae . Their viral particles have envelope composed of proteins a lipid bilayer acquired from budding through endoplasmic reticulum (ER). The phospholipid content ER membrane differs that plasma (PM). phospholipase A 2 (PLA ) superfamily consists large number members specifically catalyse hydrolysis phospholipids at particular position. Here we show CM-II isoform secreted PLA obtained...
Hepatitis C virus (HCV) is a major cause of hepatocellular carcinoma (HCC). However, the mechanisms underlying HCV-related hepatocarcinogenesis are not fully understood. The Hippo signaling pathway plays crucial role in tumor suppression. Here, we investigated pathogenesis. We demonstrated that HCV infection induces degradation LATS1, key regulator pathway. LATS1 was reversed by proteasomal inhibitor, but lysosomal inhibitors, indicating promotes LATS1. Cell-based ubiquitylation assays...
This prospective study aimed to determine whether patients with endometriosis are having different level of interlukin‐17 (IL‐17) in peritoneal fluid when compared without endometriosis. The minimal/mild had a significantly higher IL‐17 those moderate/severe or concentration was and infertility coexist. However, the did not correlate phase menstrual cycle Our suggested that might play an important role pathogenesis early endometriosis‐associated infertility.
Persistent infection with hepatitis C virus causes serious liver diseases, such as chronic hepatitis, hepatic cirrhosis and hepatocellular carcinoma. The male gender is one of the critical factors in progression fibrosis due to HCV infection; thus female hormones may play a role delaying fibrosis. It has also been reported that women are more likely than men clear acute phase infection. These observations lead present authors question: do inhibit infection? In this study using J6/JFH1...
Group A rotavirus (RVA) is the most important cause of severe gastroenteritis among children worldwide, and effective RVA vaccines have been introduced in many countries. Here we performed a molecular epidemiological analysis infection pediatric patients East Java, Indonesia, during 2015-2018. total 432 stool samples were collected from hospitalized with acute gastroenteritis. None this cohort had immunized an vaccine. The overall prevalence was 31.7% (137/432), significantly more prevalent...
Abstract RNA interference (RNAi) is a phenomenon in which small interfering (siRNA), an duplex 21 to 23 nucleotides (nt) long, or short hairpin (shRNA) resembling siRNA, mediates degradation of the target molecule sequence‐specific manner. RNAi now expected be useful therapeutic strategy for hepatitis C virus (HCV) infection. In present study we compared efficacy number shRNAs directed against different regions HCV genome, such as 5′‐untranslated region (5′UTR) (nt 286 304), Core 371 389),...
Although associations of interleukin-10 (IL-10) gene promoter polymorphisms and their protein production with endometriosis risk have been reported, the correlations remain controversial. The objective this study was to determine IL-10 at -1082, -819 -592 sites in peritoneal fluid (PF) patients without endometriosis. -1082 site were detected by amplification refractory mutation system (ARMS)–PCR that genotyped restriction fragment length polymorphism (RFLP)-PCR. Protein levels PF measured...
Robust production of infectious hepatitis C virus (HCV) in cell culture was realized by using the JFH1 strain and homologous chimeric J6/JFH1 Huh-7.5 cells, a highly HCV-permissive subclone Huh-7 cells. In this study, we aimed to establish more efficient HCV-production system gain some insight into adaptation mechanisms HCV production. By serial passaging J6/JFH1-infected obtained culture-adapted variants, designated P-27, P-38 P-47. Sequence analyses revealed that adaptive mutant viruses...
ABSTRACT Hepatitis C virus (HCV) infection causes not only intrahepatic diseases but also extrahepatic manifestations, including type 2 diabetes. We previously reported that HCV replication suppresses cellular glucose uptake by downregulation of cell surface expression transporter (GLUT2) (D. Kasai et al., J. Hepatol. 50:883–894, 2009). GLUT2 mRNA levels were decreased in both RNA replicon cells and J6/JFH1-infected cells. To elucidate molecular mechanisms HCV-induced suppression gene...
Hepatitis C virus (HCV) is a major global health problem, causing chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Development of well-tolerated regimens with high cure rates fewer side effects still much needed. Recently, natural antimicrobial peptides (AMPs) are attracting more attention as biological compounds can be good template to develop therapeutic agents, including antiviral agents against variety viruses. Various AMPs have been characterized from the venom different...
Hepatitis B virus (HBV) infection is a major risk factor for the development of chronic liver diseases, including cirrhosis and hepatocellular carcinoma (HCC). A growing body evidence suggests that HBV X protein (HBx) plays crucial role in viral replication HCC development. Here, we identified peroxiredoxin 1 (Prdx1), cellular hydrogen peroxide scavenger, as novel HBx-interacting protein. Coimmunoprecipitation analysis coupled with site-directed mutagenesis revealed region from amino acids...
We previously reported that hepatitis C virus (HCV) infection activates the reactive oxygen species (ROS)/c-Jun N-terminal kinase (JNK) signaling pathway. However, roles of ROS/JNK activation in HCV life cycle remain unclear. sought to identify a novel role pathway cycle. Immunoblot analysis revealed HCV-induced promoted phosphorylation Itch, HECT-type E3 ubiquitin ligase, leading Itch. The small interfering RNA (siRNA) knockdown Itch significantly reduced extracellular infectivity titers,...
The Kelch-like ECH-associated protein 1 (Keap1)/NF-E2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway is one of the most important defense mechanisms against oxidative stress. We previously reported that a cellular hydrogen peroxide scavenger protein, peroxiredoxin 1, target gene transcription Nrf2, acts as novel HBV X (HBx)-interacting and negatively regulates hepatitis B virus (HBV) propagation through degradation RNA. This study further demonstrates Nrf2/ARE...
The effect of vitamin D level pertinent to colorectal cancer incidence, progression, or mortality risk is complicated, and study findings are mixed. Therefore, we evaluated whether serum [25-hydroxyvitamin D, 25(OH)D] associated with the incidence sporadic (CRC).This a retrospective analysis relationship between 25(OH)D CRC. Age, sex, body mass index, history polyp, disease conditions (i.e., diabetes), medications, other eight vitamins were used as confounding factors. A total 389...