A5008491370- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Viral gastroenteritis research and epidemiology
- Endoplasmic Reticulum Stress and Disease
- Hepatitis Viruses Studies and Epidemiology
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Autophagy in Disease and Therapy
- Biochemical and Molecular Research
- Viral Infectious Diseases and Gene Expression in Insects
- Chronic Lymphocytic Leukemia Research
- Heat shock proteins research
- HIV Research and Treatment
- Systemic Lupus Erythematosus Research
- RNA modifications and cancer
- Liver Diseases and Immunity
- HIV/AIDS drug development and treatment
- Congenital Anomalies and Fetal Surgery
- Microtubule and mitosis dynamics
- Pancreatic function and diabetes
- Protein Kinase Regulation and GTPase Signaling
Kobe University
2016-2025
Kobe Institute Of Computing
2024
National Center for Infectious Diseases
2011
National Institute of Infectious Diseases
1997-2010
Tohoku University
2010
Czech Academy of Sciences, Institute of Microbiology
2008
Kyoto University
1997-1999
Harvard University
1999
Nara Institute of Science and Technology
1998
Imperial Household Agency
1993
The small guanosine triphosphatases (GTPases) Cdc42 and Rac1 regulate E-cadherin-mediated cell-cell adhesion. IQGAP1, a target of Rac1, was localized with E-cadherin beta-catenin at sites contact in mouse L fibroblasts expressing (EL cells), interacted both vivo vitro. IQGAP1 induced the dissociation alpha-catenin from cadherin-catenin complex vitro vivo. Overexpression EL cells, but not cells an E-cadherin-alpha-catenin chimeric protein, resulted decrease adhesive activity. Thus, acting...
A baculovirus (Autographa californica nucleopoly- hedrovirus) vector containing a strong promoter, the CAG was developed to introduce foreign genes into mammalian cells. Recombinant baculoviruses carrying reporter gene under control of promoter were inoculated various cell lines. High-level expression observed not only in hepatocytes but also other non-hepatic lines tested. Expression detected even 14 days after infection. The infectious titre recovered decreased significantly infection,...
IQGAP1, a target of Cdc42 and Rac1 small GTPases, directly interacts with β-catenin negatively regulates E-cadherin-mediated cell-cell adhesion by dissociating α-catenin from the cadherin-catenin complex <i>in vivo</i> (Kuroda, S., Fukata, M., Nakagawa, Fujii, K., Nakamura, T., Ookubo, Izawa, I., Nagase, Nomura, N., Tani, H., Shoji, Matsuura, Y., Yonehara, Kaibuchi, K. (1998) <i>Science</i> 281, 832–835). Here we investigated how regulate IQGAP1 function. interacted amino-terminal region...
We have previously shown that IQGAP1, a recently identified target for Cdc42 and Rac1 small GTPases, showed distribution similar to of cortical actin cytoskeleton at the membrane ruffling area induced by insulin Rac1val12 (Kuroda, S., Fukata, M., Kobayashi, K., Nakafuku, Nomura, N., Iwamatsu, A., Kaibuchi, K. (1996) J. Biol. Chem. 271, 23363–23367). Here we an IQGAP1-interacting molecule with molecular mass 43 kDa (p43) from bovine brain cytosol, using glutathione S-transferase (GST)-IQGAP1...
ABSTRACT We previously reported that cells harboring the hepatitis C virus (HCV) RNA replicon as well those expressing HCV NS3/4A exhibited increased sensitivity to suboptimal doses of apoptotic stimuli undergo mitochondrion-mediated apoptosis (Y. Nomura-Takigawa, et al., J. Gen. Virol. 87:1935-1945, 2006). Little is known, however, about whether or not infection induces virus-infected cells. In this study, by using chimeric J6/JFH1 strain genotype 2a, we demonstrated induced cell death in...
Development of complementary and/or alternative drugs for treatment hepatitis C virus (HCV) infection is still much needed from clinical and economic points view. Antiviral substances obtained medicinal plants are potentially good targets to study. Glycyrrhiza uralensis G. glabra have been commonly used in both traditional modern medicine. In this study, extracts roots their components were examined anti-HCV activity using an HCV cell culture system. It was found that a methanol extract its...
Src family tyrosine kinases are involved in modulating various signal transduction pathways leading to the induction of DNA synthesis and cytoskeletal reorganization response cell-cell or cell-matrix adhesion. The critical role these regulating cellular signaling requires that their activity be tightly controlled. proteins regulated through reversible phosphorylation dephosphorylation events alter conformation kinase. We have found evidence also is by ubiquitination. Activated forms less...
Journal Article Proteomic Profiling of Lipid Droplet Proteins in Hepatoma Cell Lines Expressing Hepatitis C Virus Core Protein Get access Shigeko Sato, Sato *To whom correspondence should be addressed. Tel: +81-3-5285-1111, Fax: +81-3-5285-1157, E-mail: fuka@nih.go.jp Search for other works by this author on: Oxford Academic PubMed Google Scholar Masayoshi Fukasawa, Fukasawa Yoshio Yamakawa, Yamakawa Tohru Natsume, Natsume Tetsuro Suzuki, Suzuki Ikuo Shoji, Shoji Hideki Aizaki, Aizaki Tatsuo...
Hepatitis C virus (HCV) core protein is a major component of viral nucleocapsid and multifunctional involved in pathogenesis hepatocarcinogenesis. We previously showed that the HCV degraded through ubiquitin-proteasome pathway. However, molecular machinery for ubiquitylation unknown. Using tandem affinity purification, we identified ubiquitin ligase E6AP as an core-binding protein. was found to bind vitro vivo promote its degradation hepatic nonhepatic cells. Knockdown endogenous by RNA...
ABSTRACT Chronic hepatitis C virus (HCV) infection is often associated with type 2 diabetes. However, the precise mechanism underlying this association still unclear. Here, using Huh-7.5 cells either harboring HCV-1b RNA replicons or infected HCV-2a, we showed that HCV transcriptionally upregulated genes for phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase), rate-limiting enzymes hepatic gluconeogenesis. In way, enhanced cellular production of 6-phosphate (G6P)...
Abstract Background Hepatitis C virus (HCV) is a major cause of liver disease and potential substantial morbidity mortality worldwide. The overall prevalence HCV infection 2%, representing 120 million people Current standard treatment using pegylated interferon ribavirin effective in only 50% the patients infected with genotype 1, associated significant side effects. Therefore, it still importance to develop new drugs for HCV. Antiviral substances obtained from natural products, including...
The development of complementary and/or alternative drugs for treatment hepatitis C virus (HCV) infection is still needed. Antiviral compounds in medicinal plants are potentially good targets to study. Morinda citrifolia a common plant distributed widely Indo-Pacific region; its fruits and leaves food sources also used as traditional medicine. In this study, using HCV cell culture system, it was demonstrated that methanol extract, n-hexane, ethyl acetate fractions from M. possess anti-HCV...
Although there is accumulating evidence that increased formation of reactive nitrogen species in cerebral vasculature contributes to the progression ischemic damage, but underlying molecular mechanisms remain elusive. Peroxiredoxin 1 (Prx1) can initiate antioxidant response by scavenging free radicals. Therefore, we tested hypothesis Prx1 regulates susceptibility nitrosative stress damage during ischemia vitro and vivo.
Abstract Signal-peptide peptidase (SPP) is an intramembrane protease that participates in the production of mature core protein hepatitis C virus (HCV). Here we show SPP inhibition reduces infectious HCV particles and pathogenesis. The immature produced SPP-knockout cells or by treatment with inhibitor quickly degraded ubiquitin–proteasome pathway. Oral administration to transgenic mice expressing (CoreTg) expression ameliorates insulin resistance liver steatosis. Moreover,...
Background & Aims: Hepatitis B virus (HBV) leads to severe liver diseases, such as cirrhosis and hepatocellular carcinoma. Identification of host factors that regulate HBV replication can provide new therapeutic targets. The discovery sodium taurocholate cotransporting polypeptide (NTCP) an entry receptor has enabled the establishment hepatic cell lines for analyzing infection propagation. Using this system, studies aimed at identifying propagation have increased. Approach Results: We...
We have previously reported on the ubiquitylation and degradation of hepatitis C virus core protein. Here we demonstrate that proteasomal protein is mediated by two distinct mechanisms. One leads to polyubiquitylation, in which lysine residues N-terminal region are preferential sites. The other independent presence ubiquitin. Gain- loss-of-function analyses using lysineless mutants substantiate hypothesis proteasome activator PA28gamma, a binding partner core, involved ubiquitin-independent...
Persistent infection with hepatitis C virus causes serious liver diseases, such as chronic hepatitis, hepatic cirrhosis and hepatocellular carcinoma. The male gender is one of the critical factors in progression fibrosis due to HCV infection; thus female hormones may play a role delaying fibrosis. It has also been reported that women are more likely than men clear acute phase infection. These observations lead present authors question: do inhibit infection? In this study using J6/JFH1...
Group A rotavirus (RVA) is the most important cause of severe gastroenteritis among children worldwide, and effective RVA vaccines have been introduced in many countries. Here we performed a molecular epidemiological analysis infection pediatric patients East Java, Indonesia, during 2015-2018. total 432 stool samples were collected from hospitalized with acute gastroenteritis. None this cohort had immunized an vaccine. The overall prevalence was 31.7% (137/432), significantly more prevalent...
Robust production of infectious hepatitis C virus (HCV) in cell culture was realized by using the JFH1 strain and homologous chimeric J6/JFH1 Huh-7.5 cells, a highly HCV-permissive subclone Huh-7 cells. In this study, we aimed to establish more efficient HCV-production system gain some insight into adaptation mechanisms HCV production. By serial passaging J6/JFH1-infected obtained culture-adapted variants, designated P-27, P-38 P-47. Sequence analyses revealed that adaptive mutant viruses...