Abstract Changes in DNA copy number contribute to cancer pathogenesis. We now show that high-density single nucleotide polymorphism (SNP) arrays can detect alterations. By hybridizing genomic representations of breast and lung carcinoma cell line tumor SNP arrays, measuring locus-specific hybridization intensity, we detected both known novel amplifications homozygous deletions these samples. Moreover, by combining genotyping with quantitation, could distinguish loss heterozygosity events...

Sensitive DNA detection is extremely important in clinical diagnostics, gene therapy, and a variety of biomedical studies. We have developed novel bioanalysis method with 0.8 fM (0.8 × 10-15 M) limit using bioconjugated fluorescent nanoparticle-based sandwich assay. An organic dye silica nanoparticle synthesized modified reverse microemulsion. The nanoparticles are highly fluorescent, photostable, easy for bioconjugation bioanalysis. They exhibit an excellent signaling ability the presence...

A new synthesis method to produce various organic‐dye‐doped silica nanoparticles using a reverse microemulsion has been developed. These show extremely high fluorescent signals (see Figure), excellent photostability, and size uniformity tenability. dye‐doped can be used for applications, especially in ultra‐sensitive bioanalysis biomedical monitoring.

The collection and then the separation of rare DNA/mRNA targets with single-base mismatches in a complex matrix is critically important human disease diagnostics, gene expression studies, profiling. major result this work development application novel genomagnetic nanocapturer (GMNC) for collection, separation, detection trace amounts DNA/RNA molecules one difference. GMNC constructed by bioconjugating molecular beacon DNA probes onto magnetic nanoparticle surfaces. We have successfully...

Abstract Background Epithelial-mesenchymal transition (EMT) has been associated with the angiogenesis and oncogenic phenotypes of multiple malignant tumors including bladder cancer (BCa). Circular RNAs (circRNAs) are recognized as crucial regulators in EMT. This study aims to illustrate possible role circular RNA_0000658 (circ_0000658) BCa underlying molecular mechanism. Methods The expression circ_0000658, microRNA (miR)-498, high mobility group AT-hook 2 (HMGA2) was assessed adjacent...

Background/Aims: Gastric cancer (GC) is a common and lethal malignancy, AMP-activated protein kinase-related kinase 5 (ARK5) has been discovered to promote metastasis in certain types of cancer. In this study, we explored the role ARK5 GC invasion metastasis. Methods: epithelial-mesenchymal transition (EMT)-related markers were determined by immunohistochemistry western blot specimens. Other methods including stably transfected against into SGC7901 AGS cells, blot, migration assays vitro...

Abstract Bladder cancer is the most common malignancy with high recurrence. Currently, long noncoding RNAs (lncRNAs) have been suggested to play vital roles in pathogenesis of bladder cancer. The present study investigated role lncRNA MIR503 host gene (MIR503HG) by using both vitro and vivo functional assays. expression MIR503HG was downregulated tissues cell lines. Low associated advanced tumor stage, histological grade, lymph node metastasis. Ectopic inhibited proliferation, growth,...

Renal cell carcinoma (RCC) has been regarded as one of the most malignant tumor types. Chemotherapy (such sorafenib) is used common strategy for treating RCC. To date, whether long noncoding RNA KIF9-AS1 involved in RCC progression and drug resistance remains unknown. In this investigation, we detected gene expression levels by western blot RT-qPCR. MTT TUNEL experiments were to show viability apoptosis, respectively. overexpression led enhanced viability, increased IC50 value sorafenib,...

We have isolated mutations defining a new gene, tlp-1, that affect asymmetric cell fates and morphogenesis during the development of C. elegans tail. tlp-1 cause defects in specification descendants T blast cell, whose polarity is controlled by Wnt signaling abnormal male tail leading to formation posterior protrusion reminiscent ‘leptoderan’, or pointy tailed, nematode species. In wild-type males, which ‘peloderan’ rounded tail, retraction tip hypodermis involves temporally ordered set...

Long non‑coding (lnc)RNA NCK1 antisense RNA 1 (NCK1‑AS1) has been characterized as an oncogene in cervical cancer, while its role prostate cancer (PC) remains unknown. It was revealed the present study that plasma NCK1‑AS1 upregulated patients with PC when compared benign prostatic hyperplasia (BPH) and healthy controls. Upregulation of distinguished from BPH Overexpression led to significantly transforming growth factor (TGF)‑β1, TGF‑β1 overexpression failed affect cells. promoted migration...

ZMYND8, an epigenetic regulator, was identified as a common oncogene across various tumors. However, little reported about the association between ZMYND8 and bladder cancer. Besides, aberrant mechanisms that contribute to abnormal expressions still remain unclear. In current study, we first found protein levels were significantly elevated in Bca samples versus normal tissues, but not mRNA levels. We then utilized Cell Counting Kit-8 (CCK-8) assay, clone formation assay transwell analysis...

As one of the prime applications liquid biopsy, detection tumor-derived whole cells and molecular markers is enabled in a noninvasive means before symptoms or hints from imaging procedures used for cancer screening. However, biopsy not diagnostic test malignant diseases per se because it fails to establish definitive diagnosis. Although single-cell genomics provides genome-wide genetic alternation landscape, technologically challenging confirm cell malignancy suspicious body fluids due...

Ethylparaben (EP) has been shown to have estrogenic effects and can affect the normal development, longevity, reproductive system of some animals. In this study, we investigated EP in male Drosophila melanogaster using transcriptome analysis or digital gene expression profiling. We then screened differentially expressed genes (DEGs) between two groups (EP-treated control group) Drosophila, performed clustering analysis, ontology (GO) function annotation, kyoto encyclopedia genomes metabolic...

The objective of the study was to investigate relationship between IL1R2 rs34043159 and Alzheimer's disease (AD) in Chinese population.A total 500 AD patients healthy controls were recruited. SNaPshot technique used detect rs34043159.The dominant recessive models associated with or without adjustment age, gender education [dominant model, P = 0.019, odds ratio (OR) 1.42, 95% confidence interval (CI) 1.06-1.89, adjusted; 0.011, OR 0.69, CI 0.51-0.92, adjusted]. model early-onset (EOAD)...

Evidence is accumulating in estimating the potential role of human papillomavirus infection prostate carcinogenesis. However, results remain inconclusive. We measured serostatus antibodies to one high-risk papillomaviruses, 16, with a newly developed peptide microarray. Serum samples were collected from 75 untreated cancer patients, along 80 control subjects. identified 12 peptides significant differences all 241 derived 16. Our showed 16 64.0% serum samples, which significantly different...

Renal cell carcinoma (RCC) accounts for 85%&90% of all kidney tumors. Although the majority renal tumors in surgical series are malignant, a number patients do undergo nephrectomy benign lesions.1 Such minimal fat angiomyolipomas (AML) visually indistinguishable from RCC and present particular diagnostic difficulties.2,3 In this study, CT region whole mass (ROW) is advocated. METHODS Patients A retrospective study was conducted; we classified 117 into two groups, 96 were 21 AML by pathologic...

Abstract For see ChemInform in Full Text.

Traditional surgical methods have high complication rate and large injury in the resection of adult polycystic kidney. We investigated effect retroperitoneal laparoscopic kidney assisted by arterial embolization.The data patients who underwent surgery embolization from November 2015 to 2018 our hospital were retrospectively analyzed, open during same period collected. The basic data, conditions, postoperative recover situation, complications two groups compared.There was no significant...