A5078138458- Effects of Radiation Exposure
- COVID-19 Clinical Research Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Mesenchymal stem cell research
- Wound Healing and Treatments
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Long-Term Effects of COVID-19
- Liver Disease and Transplantation
- Radiation Effects and Dosimetry
- Hematopoietic Stem Cell Transplantation
- RNA and protein synthesis mechanisms
- NF-κB Signaling Pathways
- Diabetes and associated disorders
- Glutathione Transferases and Polymorphisms
- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- Heat shock proteins research
- Immune Cell Function and Interaction
- Pharmacovigilance and Adverse Drug Reactions
- Reliability and Agreement in Measurement
- Radiation Therapy and Dosimetry
- Wnt/β-catenin signaling in development and cancer
- Intramuscular injections and effects
- Connective Tissue Growth Factor Research
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2006-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2009-2024
Inserm
2011-2024
Université Paris-Saclay
2010-2023
CEA Paris-Saclay
2001-2023
Université Paris Cité
2010-2022
Assistance Publique – Hôpitaux de Paris
2017-2022
Hôpital Bichat-Claude-Bernard
2014-2022
Hôpital Beaujon
2017-2022
University of Oxford
2021
Macrophages, renowned for clearance of debris and bacteria in the wound, can also uptake signaling inhibitors to promote fibrosis.
BackgroundIn patients with noncirrhotic chronic portal vein thrombosis (PVT), the benefit of long-term anticoagulation is unknown. We assessed effects rivaroxaban on risk venous thromboembolism and hypertension-related bleeding in such patients.MethodsIn this multicenter, controlled trial, we randomly assigned PVT without major factors for to receive either 15 mg/day or no anticoagulation. The primary end point was 2-year thrombosis-free survival. Secondary points included occurrence...
Despite numerous observations linking protracted exposure to low-dose (LD) radiation and leukemia occurrence, the effects of LD irradiation on hematopoietic stem cells (HSCs) remain poorly documented. Here, we show that adult HSCs are hypersensitive irradiation. This hyper-radiosensitivity is dependent an immediate increase in levels reactive oxygen species (ROS) also promotes autophagy activation Keap1/Nrf2 antioxidant pathway. Nrf2 initially protects from detrimental ROS, but protection...
TAL1 (also known as SCL) is expressed in >40% of human T cell acute lymphoblastic leukemias (T-ALLs). encodes a basic helix-loop-helix transcription factor that can interfere with the transcriptional activity E2A and HEB during leukemogenesis; however, oncogenic pathways directly activated by are not characterized. In this study, we show that, TAL1–expressing T-ALL lines, activates NKX3.1, tumor suppressor gene required for prostate stem maintenance. NKX3.1 activation mediated...
Gault, N. and Lefaix, J-L. Infrared Microspectroscopic Characteristics of Radiation-Induced Apoptosis in Human Lymphocytes. Radiat. Res. 160, 238–250 (2003).Infrared microspectroscopic characterization radiation-induced apoptosis was used as a new analytical tool to study the kinetics human peripheral blood lymphocytes at molecular level. This vibrational technique, which has already been investigate biomolecules normal tumor cells, allows simultaneous detection biochemical changes various...
A total of 2%-10% patients with vascular liver disease (VLD) have paroxysmal nocturnal hemoglobinuria (PNH). Eculizumab reduces complement-mediated haemolytic activity in PNH. This study was aimed at assessing the impact eculizumab on VLD outcome. Retrospective cohort PNH patients, Valdig registry, who had diagnosed between 1997 and 2019 is considered. exposure interest. Studied outcomes were death, venous thrombosis, bleeding, arterial ischemic event, infection, liver-related complications....
// Anne-Sophie Gallouet 1, 2, 3, 4, 5 , Federica Ferri 5, 6, * Vanessa Petit Aude Parcelier Daniel Lewandowski Nathalie Gault Vilma Barroca Stéphanie Le Gras 6 Eric Soler 7 Frank Grosveld Irwin Davidson Paul-Henri Romeo 1 CEA/DRF/iRCM/LRTS, 92265 Fontenay-aux-Roses cedex, France 2 Inserm U967, 3 Université Paris-Diderot, Paris 7, 4 Paris-Sud, 11, Equipe labellisée Ligue contre le Cancer, Department of Functional Genomics and Institut de Génétique et Biologie Moléculaire Cellulaire,...
Fourier transform infrared microspectroscopy (FT-IRM), which allows simultaneous detection of biochemical changes in the various cellular compartments, was used as a new analytical tool to study early radiation- and oxidation-induced damage at molecular level single human cells.HaCaT keratinocytes were given dose 6 Gy (137Cs) or 650 microM H2O2, neither is cytotoxic (neutral red assay) but both result less than 10% clonogenic survival, deposited on zinc sulphur (ZnS) windows for infra-red...
Gault, N., Rigaud, O., Poncy, J-L. and Lefaix, Biochemical Alterations in Human Cells Irradiated with α Particles Delivered by Macro- or Microbeams. Radiat. Res. 167, 551–562 (2007).Irradiation of individual cell nuclei charged-particle microbeams requires accurate identification localization cells using Hoechst staining UV illumination before computer-monitored each cell. Using Fourier-transform infrared microspectroscopy (FT-IRM), we investigated whether the experimental conditions used...
Human multilineage-differentiating stress enduring (Muse) cells are nontumorigenic endogenous pluripotent-like stem that can be easily obtained from various adult or fetal tissues. Regenerative effects of Muse have been shown in some disease models. specifically home damaged tissues where they exert pleiotropic effects. Exposition the small intestine to high doses irradiation (IR) delivered after radiotherapy nuclear accident results a lethal gastrointestinal syndrome (GIS) characterized by...
A comparison of three cellular irradiation techniques using the Monte Carlo simulation toolkit Geant4 is presented in this paper. They involve electrodeposited source alpha particle-emitting radionuclides, random classical beam and single cell targeted a focused microbeam line. The allows calculation hit distributions among population as well absorbed dose for two typical geometries.
The sensitivity of chronic lymphocytic leukemia (CLL) cells to current treatments, both in vitro and vivo, relies on their ability activate apoptotic death. CLL resistant DNA damage-induced apoptosis display deregulation a specific set genes.Microarray hybridization (Human GeneChip, Affymetrix), immunofluorescent situ labeling coupled with video-microscopy recording/analyses, chromatin-immunoprecipitation (ChIP), polymerase chain reactions (PCR), real-time quantitative PCR (RT-QPCR)...
This study was designed for the histopathological, cellular and biochemical characterization of a skin lesion removed surgically from young male several months after accidental exposure to cesium-137, with an emphasis on expression transforming growth factor beta1 (TGFB1) tumor necrosis alpha (TNFA) occurrence apoptosis. Under hypertrophic epidermis, highly inhomogeneous inflammatory dermis observed, together fibroblastic proliferation in necrotic areas. Immunostaining revealed...
Gault, N., Vozenin-Brotons, M. C., Calenda, A., Lefaix, J. L. and Martin, T. Promoter Sequences Involved in Transforming Growth Factor β1 Gene Induction HaCaT Keratinocytes after Gamma Irradiation. Radiat. Res. 157, 249 – 255 (2002).Transforming growth factor beta 1 (TGFB1) is a cytokine involved the development of both acute late cutaneous radiation syndromes. We previously demonstrated that ionizing induces TGFB1 expression vivo pig skin within few hours. The purpose present study was to...
Purpose: to characterize radiation-induced apoptosis in human cells using Fourier transform infrared microspectroscopy (FT-IRM) as a new analytical tool. Material and methods: Normal circulating lymphocytes were given γ ray dose of 6 Gy, or treated with t-butyl hydroperoxide (t-BuOH). HaCaT keratinocytes 20 Gy. Cells deposited on ZnS windows for spectral acquisition 2 days h after irradiation t-BuOH treatment. Apoptosis was simultaneously assessed by flow cytometry analysis displaying...