A5072819080- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- PI3K/AKT/mTOR signaling in cancer
- Protein Kinase Regulation and GTPase Signaling
- Hepatitis C virus research
- Metabolism, Diabetes, and Cancer
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Monoclonal and Polyclonal Antibodies Research
- Asthma and respiratory diseases
- RNA Research and Splicing
- Genetic Associations and Epidemiology
- Signaling Pathways in Disease
- T-cell and B-cell Immunology
- Cancer, Hypoxia, and Metabolism
- Chronic Lymphocytic Leukemia Research
- Liver physiology and pathology
- Glycosylation and Glycoproteins Research
- Neuroblastoma Research and Treatments
- RNA modifications and cancer
- Fibroblast Growth Factor Research
- Protein Tyrosine Phosphatases
- Genomics and Rare Diseases
- Nuclear Structure and Function
- Reproductive Physiology in Livestock
Guangzhou Development Zone Hospital
2023-2024
Dalian Medical University
2024
Second Hospital of Anhui Medical University
2020-2023
Anhui Medical University
2020-2023
Dongguan People’s Hospital
2014
Jinan University
2014
Industrial Technology Research Institute
2010-2013
ITRI International
2010
National Health Research Institutes
2005-2006
Genomics Research Center, Academia Sinica
2004-2005
The large docking protein IRS-1 is a major substrate for the insulin receptor and other tyrosine kinases. It plays key role in eliciting many of insulin’s actions, including binding activation phosphatidylinositol (PI) 3-kinase subsequent increase glucose transport. Gene disruption mice associated with an impaired insulin-stimulated disposal vivo transport vitro , but survival animals residual sensitivity dependent on presence alternative IRS-2. We examined expression function IRS-2...
The hepatocyte growth factor (HGF/SF) receptor, Met, regulates mitogenesis, motility, and morphogenesis in a cell type-dependent fashion. Activation of Met via autocrine, paracrine, or mutational mechanisms can lead to tumorigenesis metastasis numerous studies have linked inappropriate expression this ligand-receptor pair most types human solid tumors. To prepare mAbs HGF/SF, mice were immunized with native denatured preparations the ligand. Recloned tested vitro for blocking activity...
The signaling molecules insulin receptor substrate (IRS)-1 and the newly described IRS-2 (4PS) molecule are major interleukin 4 (IL-4)-dependent phosphoproteins. We report here that IL-2, IL-7, IL-15, as well IL-4, rapidly stimulate tyrosine phosphorylation of IRS-1 in human peripheral blood T cells, NK lymphoid cell lines. In addition, we show Janus kinases, JAK1 JAK3, associate with cells. Coexpression studies demonstrate these kinases can tyrosine-phosphorylate IRS-2, suggesting a...
Insulin receptor substrate 2 (IRS-2) has recently been shown to be a of the insulin (IR). In this study we utilize yeast two-hybrid system and assays <i>in vitro</i> interaction demonstrate that IRS-2 interacts directly with IR insulin-like growth factor I receptor. We show that, like IRS-1, region contains putative phosphotyrosine binding SAIN elements(188-591) is sufficient for dependent upon NP<i>X</i>(p)Y (where (p)Y phosphotyrosine) motifs within juxtamembrane domains receptors....
The NH2 terminus of insulin receptor substrate-1 (IRS-1) contains a pleckstrin homology (PH) domain. We deleted the PH domain in IRS-1 (IRS-1 delta PH) and expressed mutant Chinese hamster ovary 32D cells. During stimulation, is poorly tyrosine-phosphorylated CHO cells, but undergoes serine/threonine phosphorylation. Similarly, fails to undergo insulin-stimulated tyrosine phosphorylation which uncouples activation phosphatidylinositol 3'-kinase p70s6k from endogenous receptors....
Interleukin 3-dependent murine 32D cells do not detectably express members of the ErbB receptor family and proliferate in response to known ligands for these receptors. transfectants were generated expressing human ErbB4 alone (32D.E4) or with ErbB2 (32D.E2/E4). Epidermal growth factor (EGF), neuregulin 1-beta (NRG1-beta), betacellulin (BTC), transforming factor-alpha (TGF-alpha), heparin binding-EGF (HB-EGF), amphiregulin analyzed their ability mediate mitogenesis transfectants. 32D.E4...
Abstract Wild-type (F/B), constitutively active (F/B*), and three kinase-inactive (F/Ba−, F/Bb−, F/Bc−) forms of Akt/protein kinase B (PKB) were permanently overexpressed in FDCP2 cells. In the absence insulin-like growth factor-1 (IGF-1), activities PKB, cyclic nucleotide phosphodiesterase 3B (PDE3B), PDE4 similar nontransfected cells, mock-transfected (F/V) F/B F/B− F/V IGF-1 increased PDE3B, ∼2-fold. IGF-1, a wortmannin-sensitive manner, PKB activity ∼10-fold PDE3B phosphorylation...
The Jak1, Jak2, Jak3, and Fes tyrosine kinases have been demonstrated to undergo phosphorylation in response interleukin (IL)-4 stimulation different cell systems. However, it is not clear which, if any, of these are responsible for initiating IL-4-induced intracellular substrates vivo In the present study, we utilized a mutant Jak1-deficient HeLa line, E1C3, its parental Jak1-expressing counterpart, 1D4, analyze role Jak1 mediating events. IL-4 treatment rapidly induced insulin receptor...
Pleckstrin homology (PH) domains are approximately 110 amino acid residues in length and structurally conserved a number of intracellular signaling proteins. A role for these has been postulated beta ARK, which binds to G gamma subunits. We have quantified the binding individual (His)6-tag PH human Db1, Sos1, rat IRS-1, ARK with an extra 33-residue C-terminal extension (beta + C) Our vitro studies show that all (apart from Sos1), bind subunits dose-dependent manner, but C 4 times as much at...
Few studies have paid attention to the performances of non-invasive models in diagnosing stages liver fibrosis and inflammation, which are critical for early accurate assessment prognostication decisions on antiviral treatment chronic hepatitis B infection patients with high virus DNA normal or mildly elevated alanine transaminase levels (≤2 times upper limit (ULN)). This study aimed investigate value routine serum markers evaluation inflammation these patients.A total 370 consecutive...
Background In recent years, with the development of society, children's daily exposure to screen time has gradually increased. Screen and sedentary behavior have brought a host harms lives. The aim this study was explore effects on precocious puberty early development. Methods This is cross-sectional in school-based population. A total 3,560 children were recruited from Qufu City, Shandong province using multistage stratified cluster random sampling. All subjects had physical examination by...
Signaling from the activated insulin receptor is initiated by its tyrosine phosphorylation of substrates (IRSs). The IRSs then act as docking/effector proteins for various signaling containing src homology 2 domains. Four members IRS family, designated IRS-1 through IRS-4, have been identified. Although these show considerable structural homology, extent to which they overlap in functions has not explored detail. 32D hematopoietic cell line, contains no detectable amounts any IRS, provides a...
<i>Background:</i> Upregulation of the platelet-derived growth factor receptor-α (PDGFRα) in airway myofibroblast cells is one mechanisms remodeling. The genetic association between PDGFRα promoter polymorphism and severity childhood asthma was examined. <i>Methods:</i> Five single nucleotide polymorphisms (SNPs) at regions gene were genotyped 277 unrelated allergic nonallergic asthmatic children 93 age-matched controls. Promoter haplotypes constructed using SNP...
Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a serious medical condition with high short-term mortality rate, making accurate prognostic assessment essential for informed clinical decision-making. In this study, we aimed to develop simple and effective model predicting in patients HBV-ACLF.To achieve our objective, enrolled both cross-sectional cohort (n = 291) retrospective 185) study. We collected laboratory data from these cohorts performed univariate...