A5079550455- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Carbohydrate Chemistry and Synthesis
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Antimicrobial Resistance in Staphylococcus
- Cystic Fibrosis Research Advances
- Escherichia coli research studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Nicotinic Acetylcholine Receptors Study
- Trace Elements in Health
- Lipid Membrane Structure and Behavior
- Clostridium difficile and Clostridium perfringens research
- Blood groups and transfusion
- Neuroscience and Neuropharmacology Research
- Seaweed-derived Bioactive Compounds
- Plant-Microbe Interactions and Immunity
- Connexins and lens biology
- Cell Image Analysis Techniques
Michigan State University
2019-2024
Michigan United
2023
Physical Sciences (United States)
2023
To counteract infection with phage, bacteria have evolved a myriad of molecular defense systems. Some these systems initiate process called abortive infection, in which the infected cell kills itself to prevent phage propagation. However, such must be inhibited absence spurious death host. Here, we show that cyclic oligonucleotide based anti-phage signaling system (CBASS) accomplishes this by sensing intracellular folate molecules and only expressing group. These results enhance our...
Anxiety disorders are highly prevalent but little is known about their underlying mechanisms. Gap junctions exist in brain regions important for anxiety regulation, such as the ventral hippocampus (vHIP) and mPFC, functions these areas have not been investigated. Using pharmacological blockade of neuronal gap combined with electrophysiological recordings, we found that play a role theta rhythm vHIP mPFC adult mice. Bilateral infusion junction blockers into decreased anxiety-like behavior on...
<ns4:p>Cholera infections caused by the gamma-proteobacterium <ns4:italic>Vibrio cholerae </ns4:italic>have ravaged human populations for centuries, and cholera pandemics have afflicted every corner of globe. Fortunately, interventions such as oral rehydration therapy, antibiotics/antimicrobials, vaccines saved countless people with cholera, new probiotics phage therapy are being developed promising approaches to treat even more infections. Although current therapies mostly effective can...
Significance Form follows function is true of living organisms, including bacteria, as the shapes and morphology they adopt contribute to their biological characteristics. But whether bacteria can actively change shape adapt environment less understood. Vibrio cholerae , pathogenic bacterium responsible for diarrheal disease cholera, adopts a characteristic “comma”-shaped cell morphology. Here we show that intracellular signaling molecule, cyclic di-GMP, drives curved V. straight...
Staphylococcus aureus is a significant human pathogen due to its capacity cause multitude of diseases. As such, S. efficiently pillages vital nutrients from the host; however, molecular mechanisms that support sulfur acquisition during infection have not been established. One most abundant extracellular sulfur-containing metabolites within host cysteine, which acts as major redox buffer in blood by transitioning between reduced and oxidized (cystine) forms. We therefore hypothesized acquires...
A leading cause of bacterial gastroenteritis, Campylobacter jejuni is also associated with broad sequelae, including extragastrointestinal conditions such as reactive arthritis and Guillain-Barré Syndrome (GBS). CbrR a C. response regulator that annotated diguanylate cyclase (DGC), an enzyme catalyzes the synthesis c-di-GMP, universal second messenger, from GTP. In DRH212, we constructed unmarked deletion mutant, cbrR-, complemented cbrR+. Motility assays indicated hyper-motile phenotype...
SUMMARY The El Tor biotype of Vibrio cholerae is responsible for perpetuating the longest cholera pandemic in recorded history (1961-current). genomic islands VSP-1 and -2 are two understudied genetic features that distinguish from previous pandemics. To understand their utility, we calculated co-occurrence VSP genes across bacterial genomes. This analysis predicted previously uncharacterized vc0175 , herein renamed d eoxycytidylate eaminase V ibrio ( dcdV ), a gene network with dncV cyclic...
c-di-GMP has been widely recognized for its essential role in the production of exopolysaccharides bacteria, such as alginate produced by Pseudomonas and Azotobacter spp. This study reveals that levels also affect physical properties alginate, favoring high-molecular-mass alginates response to lower OTRs. finding opens up new alternatives design tailor-made biotechnological applications.
Dickeya dadantii is a phytopathogenic bacterium that causes diseases on wide range of host plants. The pathogen secretes pectate lyases (Pel) through the type II secretion system (T2SS) degrades cell wall in virulence
The genus Azotobacter, belonging to the Pseudomonadaceae family, is characterized by formation of cysts, which are metabolically dormant cells produced under adverse conditions and able resist desiccation. Although this developmental process has served as a model for study cell differentiation in Gram-negative bacteria, molecular basis its regulation still poorly understood. Here, we report that ubiquitous second messenger cyclic dimeric GMP (c-di-GMP) critical cysts Azotobacter vinelandii...
Abstract A major challenge faced by bacteria is infection bacteriophage (phage). Abortive one strategy for combating phage in which an infected cell kills itself to limit replication, thus protecting neighboring kin. One class of abortive systems the c yclic oligonucleotide b ased a nti-phage s ignaling ystem (CBASS) relies on two core enzymatic activities; oligo-nucleotide cyclase that activated following and cyclic-oligo-nucleotide sensitive effector whose activity cell. However,...
Toxin-antitoxin (TA) systems are ubiquitous two-gene loci that bacteria use to regulate cellular processes such as phage defense. Here, we demonstrate the mechanism by which a novel type III TA system, avcID , is activated and confers resistance infection. The toxin of system (AvcD) deoxycytidylate deaminase converts deoxycytidines (dC) dexoyuridines (dU), while RNA antitoxin (AvcI) inhibits AvcD activity. We have shown deaminated dC nucleotides upon infection, but molecular was unknown....
Toxin-antitoxin (TA) systems are ubiquitous two-gene loci that bacteria use to regulate cellular processes such as phage defense. Here, we demonstrate the mechanism by which a novel type III TA system, avcID , is activated and confers resistance infection. The toxin of system (AvcD) deoxycytidylate deaminase converts deoxycytidines (dC) dexoyuridines (dU), while RNA antitoxin (AvcI) inhibits AvcD activity. We have shown deaminated dC nucleotides upon infection, but molecular was unknown....
AMERICAN SOCIETY OF PLASTIC SURGEONS & RECONSTRUCTIVE SURGERY PRS GLOBAL OPEN ASPS EDUCATION NETWORK
Cyclic-di-GMP (c-di-GMP) is a critical bacterial second messenger that enables the physiological phase transition in Erwinia amylovora, phytopathogenic bacterium causes fire blight disease. C-di-GMP generation dependent on diguanylate cyclase enzymes while degradation of c-di-GMP can occur through action phosphodiesterase (PDE) contain an active EAL and/or HD-GYP domain. The HD-GYP-type PDEs, which are absent E. directly degrade into two GMP molecules. PDEs domain, as found all pGpG....
Abstract The cell morphology of rod-shaped bacteria is determined by the rigid net peptidoglycan forming wall. While V. cholerae grows into a curved shape under most conditions, straight rods have been observed. However, signals and regulatory pathways controlling transitions in benefits switching between rod not determined. We demonstrate that regulated bacterial second messenger cyclic dimeric guanosine monophosphate (c-di-GMP) repressing expression crvA , gene encoding an intermediate...
Abstract East Lansing, MI 48824 Cyclic di‐nucleotides (CDNs) are central signaling molecules in organisms spanning the tree of life. In bacteria, CDNs mediate many important physiological functions such as biofilm formation, motility, and virulence. also implicated activation cellular biological defense systems both bacteria eukaryotes. CDN 3’3’‐cyclic GMP‐AMP (3’3’‐cGAMP) activates a putative phage system Vibrio cholerae controls processes Geobacter sulfurreducens Bdellovibrio bacteriovorus...