A5018504905- S100 Proteins and Annexins
- Neonatal Respiratory Health Research
- Advanced Glycation End Products research
- Inflammation biomarkers and pathways
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Biomarkers in Disease Mechanisms
- Bioinformatics and Genomic Networks
- Cannabis and Cannabinoid Research
- Gene expression and cancer classification
- Pancreatic function and diabetes
- Computational Drug Discovery Methods
- Protease and Inhibitor Mechanisms
- Polyamine Metabolism and Applications
- Gene Regulatory Network Analysis
- Tuberculosis Research and Epidemiology
- Immune Response and Inflammation
- DNA Repair Mechanisms
- Fungal and yeast genetics research
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- RNA and protein synthesis mechanisms
- Essential Oils and Antimicrobial Activity
- Wnt/β-catenin signaling in development and cancer
University of Colorado Anschutz Medical Campus
2022-2024
Rutgers, The State University of New Jersey
2014-2024
Rutgers New Jersey Medical School
2024
Virginia Tech
2022-2023
Michigan State University
2019-2022
Michigan United
2022
The Ohio State University Wexner Medical Center
2015-2016
The Ohio State University
2012-2016
Ohio University
2012-2016
Cancer Genetics (United States)
2011-2012
Abstract RAGE is a multifunctional receptor implicated in diverse processes including inflammation and cancer. In this study, we report that expression upregulated widely aggressive triple-negative breast cancer (TNBC) cells, both primary tumors lymph node metastases. evaluating the functional contributions of cancer, found RAGE-deficient mice displayed reduced propensity for tumor growth. an established model lung metastasis, systemic blockade by injection neutralizing antibody inhibited...
S100A7/psoriasin, a member of the epidermal differentiation complex, is widely overexpressed in invasive estrogen receptor (ER)α-negative breast cancers. However, it has not been established whether S100A7 contributes to cancer growth or metastasis. Here, we report consequences its expression on inflammatory pathways that impact growth. Overexpression human murine homologue mS100a7a15 enhanced cell proliferation and upregulated various proinflammatory molecules ERα-negative cells. To examine...
Vitamin D has long been linked to resistance tuberculosis, an infectious respiratory disease that is increasingly hard treat because of multidrug resistance. Previous work established vitamin induces macrophage antimicrobial functions against Mycobacterium tuberculosis. In this article, we report a novel, metabolic role for in tuberculosis identified through integrated transcriptome and mechanistic studies. Transcriptome analysis revealed association between receptor (VDR) lipid metabolism...
Abstract Accessory sigma factors, which reprogram RNA polymerase to transcribe specific gene sets, activate bacterial adaptive responses noxious environments. Here we reconstruct the complete factor regulatory network of human pathogen Mycobacterium tuberculosis by an integrated approach. The approach combines identification direct interactions between M. factors in E. coli model system, validation selected links , and extensive literature review. resulting comprises 41 among all 13 factors....
JWH‐015, a cannabinoid receptor 2 (CB2) agonist has tumor regressive property in various cancer types. However, the underlying mechanism by which it acts lung is still unknown. Tumor associated macrophage (TAM) intensity positive correlation with progression. Also, macrophages recruited at site promote growth enhancing epithelial to mesenchymal (EMT) In this study, we analyzed role of JWH‐015 on EMT and infiltration regulation EGFR signaling. inhibited NSCLC cells A549 also reversed nature...
// Janani Ravi 1 , Amita Sneh Konstantin Shilo Mohd W. Nasser and Ramesh K. Ganju Department of Pathology, The Ohio State University, Ohio, USA. Correspondence: Nasser, email: K Ganju, Keywords : NSCLC, EGFR, Met-F-AEA, FAAH Received December 19, 2013 Accepted February 2014 Published 21, Abstract endocannabinoid anandamide (AEA), a neurotransmitter was shown to have anti-cancer effects. Fatty acid amide hydrolase (FAAH) metabolizes AEA decreases its anti-tumorigenic activity. In this study,...
// Mohamad Elbaz 1, 2 , Dinesh Ahirwar 1 Janani Ravi Mohd W. Nasser Ramesh K. Ganju Department of Pathology and The Comprehensive Cancer Center, Ohio State University, Wexner Medical Columbus, OH, USA Pharmacology, Pharmacy School, Helwan Helwan, Egypt Correspondence to: Ganju, email: Ramesh.ganju@osumc.edu Keywords: EGFR, IGF-IR, CNR2 Abbreviations: epidermal growth factor receptor (EGFR), insulin-like receptor-I (IGF-IR), estrogen (ER), cannabinoid receptor-2 (CNR2), matrix...
Bacterial phage shock protein (PSP) systems stabilize the bacterial cell membrane and protect against envelope stress. These have been associated with virulence, but despite their critical roles, PSP components are not well characterized outside proteobacteria. Using comparative genomics sequence-structure-function analyses, we systematically identified analyzed homologs, phyletic patterns, domain architectures, gene neighborhoods. This approach underscored evolutionary significance of...
The bacterial envelope integrates essential stress-sensing and adaptive functions; thus, envelope-preserving functions are important for survival. In Gram-negative bacteria, integrity during stress is maintained by the multi-gene Psp response. Mycobacterium tuberculosis was thought to lack system since it encodes only pspA no other psp ortholog. Intriguingly, maps downstream from clgR, which a transcription factor regulated MprAB-σ(E) envelope-stress-signaling system. clgR inactivation...
Wnt ligands are considered classical morphogens, for which the strength of cellular response is proportional to concentration ligand. Herein, we show an emergent property bistability arising from feedback among destruction complex proteins that target key transcriptional co-activator β-catenin degradation. Using biochemical reconstitution, identified positive between scaffold protein Axin and kinase glycogen synthase 3 (GSK3). Theoretical modeling this GSK3 suggested activity exhibits...
Sulfur is an indispensable element for bacterial proliferation. Prior studies demonstrated that the human pathogen Staphylococcus aureus utilizes glutathione (GSH) as a source of nutrient sulfur; however, mechanisms GSH acquisition are not defined. Here, we identify five-gene locus comprising putative ABC-transporter and predicted γ–glutamyl transpeptidase ( ggt ) promotes S . proliferation in medium supplemented with either reduced or oxidized (GSSG) sole sulfur. Based on these phenotypes,...
The basis of several recent methods for drug repurposing is the key principle that an efficacious will reverse disease molecular 'signature' with minimal side effects. This was defined and popularized by influential 'connectivity map' study in 2006 regarding reversal relationships between disease- drug-induced gene expression profiles, quantified a disease-drug score.' Over past 15 years, studies have proposed variations calculating connectivity scores toward improving accuracy robustness...
Staphylococcus aureus is a significant human pathogen due to its capacity cause multitude of diseases. As such, S. efficiently pillages vital nutrients from the host; however, molecular mechanisms that support sulfur acquisition during infection have not been established. One most abundant extracellular sulfur-containing metabolites within host cysteine, which acts as major redox buffer in blood by transitioning between reduced and oxidized (cystine) forms. We therefore hypothesized acquires...
The bioactive form of vitamin D, 1α, 25-dihydroxyvitamin D3 (1α, 25(OH)2D3), is a secosteroid hormone that binds to the D receptor (VDR), member nuclear super-family expressed in many cell types, and modulates variety biological functions. 25(OH)2D3 essential for bone mineral homeostasis, but also regulates growth differentiation multiple displays immunoregulatory anti-inflammatory activities. antiproliferative, prodifferentiative, antibacterial, immunomodulatory properties synthetic VDR...
Studying proteins through the lens of evolution can reveal conserved features, lineage-specific variants, and their potential functions. MolEvolvR (https://jravilab.org/molevolvr) is a novel web-app enabling researchers to visualize molecular interest in phylogenetic context across tree life, spanning all superkingdoms. The accepts multiple input formats — protein/domain sequences, homologous proteins, or domain scans and, using general-purpose computational workflow, returns detailed...
A fundamental requirement for life is the replication of an organism's DNA. Studies in Escherichia coli and Bacillus subtilis have set paradigm DNA bacteria. During initiation E. B. subtilis, replicative helicase loaded onto at origin by ATPase loader. However, most bacteria do not encode homologs to loaders subtilis. Recent work has identified DciA protein as a predicted operator that may perform function analogous proteins, which are defined presence DUF721 domain (termed herein),...
SUMMARY The El Tor biotype of Vibrio cholerae is responsible for perpetuating the longest cholera pandemic in recorded history (1961-current). genomic islands VSP-1 and -2 are two understudied genetic features that distinguish from previous pandemics. To understand their utility, we calculated co-occurrence VSP genes across bacterial genomes. This analysis predicted previously uncharacterized vc0175 , herein renamed d eoxycytidylate eaminase V ibrio ( dcdV ), a gene network with dncV cyclic...
Abstract Bacterial phage shock protein (PSP) systems stabilize the bacterial cell membrane and protect against envelope stress. These have been associated with virulence, but despite their critical roles, PSP components are not well-characterized outside proteobacteria. Using comparative genomics sequence-structure-function analyses, we systematically identified analyzed homologs, phyletic patterns, domain architectures, gene neighborhoods. This approach underscored evolutionary significance...