Obesity is associated with adipose tissue inflammation, which has been attributed to changes in the number and types of leukocytes tissue. Exercise training thought be important for reduction but mechanisms by this may occur are incompletely understood. Here, we evaluated effect exercise on several inflammation-associated tissue, including infiltration inflammatory macrophages T cells.Four-week-old male C57BL/6J mice were randomly assigned four groups that received a normal diet (ND) plus...

Kaempferol (KMP) is an important flavonoid in many fruits and vegetables. Preclinical studies on KMP have reported its pharmacological effects, including antimicrobial, antioxidant, anti-inflammatory, antitumor, antidiabetic, myocardial protective, neuroprotective effects. Additionally, some epidemiological revealed a negative association between the consumption of KMP-containing foods risk developing several disorders, such as cancer cardiovascular diseases. Thus, although large body...

Purpose The infiltration of macrophages in skeletal muscle during exhaustive exercise promotes inflammation, myofiber lesion, and injury. Although neutrophils upregulate macrophage muscles exercise, the role promoting injury after remains unclear. In this study, we investigated effects preexercise neutrophil depletion with antineutrophil antibody treatment on injury, exercise. Methods Male C57BL/6J mice were randomly assigned to four groups, namely, sedentary control (n = 10), 10). given...

Exhaustive exercise promotes muscle injury, including myofiber lesions; however, its exact mechanism has not yet been elucidated. In this study, we tested the hypothesis that macrophage depletion by pretreatment with clodronate liposomes alters injury and inflammation following exhaustive exercise. Male C57BL/6J mice were divided into four groups: rest plus control liposome (n=8), (n=8). Mice treated or for 48 h before undergoing on a treadmill. Twenty-four hours after exercise,...

Kaempferol (KMP) has numerous important biological functions, and we recently showed that it remarkably increased intracellular adenosine triphosphate (ATP) content in C2C12 myotubes under hypoxic conditions. Since ATP is generated by aerobic energy metabolism or anaerobic glycolysis, hypoxia inducible factor-1α (HIF-1α) been shown to be associated with metabolic remodeling causes shift from glycolysis response Here, investigate the effects of KMP conditions on stabilization HIF-1α its...

The innate immune system is associated with the development of local inflammation. Neutrophils play an essential role in adipose tissue (AT) inflammation obesity by producing elastase, which can promote activation and infiltration macrophages. Exercise training attenuates AT via suppression macrophage infiltration. However, mechanisms driving this phenomenon remains to be elucidated. Here, we evaluated effects exercise on neutrophils elastase expression obese mouse model. Four-week-old male...

Abstract Prolonged intense exercise induces acute renal injury; however, the precise mechanism remains unclear. We investigated effects of neutrophil depletion in male C57BL/6J mice. Male mice were divided into four groups: sedentary with control antibody; antineutrophil exhaustive and antibody. Antineutrophil (1A8) or antibody was administered i.p . to before they ran on a treadmill. Plasma levels creatinine blood urea nitrogen (BUN) measured. Renal histology assessed 24 h after exercise,...

Although nonalcoholic steatohepatitis (NASH) is an important component of the metabolic syndrome, scavenger receptor CD36 also modulates NASH development. This study aimed to clarify whether exercise training suppresses expression in a mouse model NASH. Male C57BL/6 mice were divided into four groups: normal diet (ND) sedentary, ND exercise, high-fat and high-fructose water (HFF) HFF groups. The groups trained on motorized treadmill at running speeds 15–20 m/min for 60 min/day, 5 times/week...

Kaempferol (KMP), a flavonoid in edible plants, exhibits diverse pharmacological effects. Growing body of evidence associates extended lifespan with physical activity (PA) and sleep, but KMP's impact on these behaviors is unclear. This double-blind, placebo-controlled, crossover trial assessed effects PA sleep.

Hypoxic conditions in the body have been reported to occur aging and exercising muscles. It has suggested that a reduction ATP, due hypoxia, may contribute related declines muscle performance. In this study, we established hypoxic assay systems evaluated effects of 65 phytochemicals on intracellular ATP content C2C12 myotubes, more accurately determine their physiological activities. We found myotubes be reduced, under conditions. However, kaempferol markedly increased it systems, by...

ABSTRACT Plants exposed to hypoxic conditions have been suggested produce more biologically active phytochemicals than those normal oxygen levels. Previously, we investigated 314 highland crop species and showed that the flavonoid kaempferol extracted from a quinoa grain markedly increased mitochondrial metabolism ATP production in environment vitro. Thus, hypothesized would be effective during exercise under harsh conditions, which anaerobic occurs. This study adopted double‐blind,...

Hypoxia can occur in daily life, and exercise is a potential trigger. Intense increases oxygen demand muscles, potentially compromising performance by reducing ATP resynthesis. Kaempferol (KMP) intracellular resynthesis hypoxic cellular models. We investigated the effects of KMP on utilization during general human population. Study 1 (Ramp-incremental cycling test): Overall, 20 men were randomized to four groups administrated placebo, 2.5, 10, or 25 mg for 8 days crossover design....

Prolonged exercise induces acute renal injury; however, the precise mechanism remains unclear. We investigated effects of neutrophil depletion in male C57BL/6J mice. Male mice were divided into four groups: sedentary with control antibody, antineutrophil exhaustive and antibody. Antineutrophil (1A8) or antibody was administered intraperitoneally to prior their running on a treadmill. Renal histology assessed 24 h after exercise, concentration kidney injury molecule (KIM)-1 measured using...

Exhaustive exercise is known to induce acute renal damage. However, the precise mechanisms remain unclear. We investigated effects of macrophage depletion on exhaustive exercise-induced Male C57BL/6 J mice were divided into four groups: sedentary with control liposome (n=8), clodronate and (n=8). Mice treated liposomes or intraperitoneally for 48 h before undergoing exercise. Renal function histology tested at 24 h. The expression levels kidney injury molecule (KIM)-1 inflammatory cytokines...

Exhaustive exercise induces acute liver stress; however, the precise mechanisms remain unclear.We investigated effects of neutrophil depletion in male C57BL/6J mice. Male mice were divided into four groups: sedentary with control antibody ( n = 20), antineutrophil exhaustive and 20). Antineutrophil antibodies (1A8) or administered intraperitoneally before running on a treadmill. Immediately at 24 h after to exhaustion treadmill 7% gradient speed m·min -1 , blood counts measured by flow...

Obesity is associated with the pathogenesis of chronic inflammatory diseases, such as type II diabetes and nonalcoholic steatohepatitis, which in turn have been attributed to inflammation visceral adipose tissue liver. The innate immune system via macrophages, neutrophils lymphocytes related development inflammation. Exercise has anti-inflammatory effects may prevent diseases. To elucidate mechanisms improvement and/or prevention local due exercise important ensure effective therapy. This...