A5055602609- Virus-based gene therapy research
- Skin and Cellular Biology Research
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- RNA regulation and disease
- Neurogenetic and Muscular Disorders Research
- Cell Adhesion Molecules Research
- Dermatological and Skeletal Disorders
- Animal Genetics and Reproduction
- Lysosomal Storage Disorders Research
- Milk Quality and Mastitis in Dairy Cows
- Endoplasmic Reticulum Stress and Disease
- Biomedical and Engineering Education
- Viral Infections and Immunology Research
- Electrospun Nanofibers in Biomedical Applications
- Infant Nutrition and Health
- Hair Growth and Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Trace Elements in Health
- Developmental Biology and Gene Regulation
- MicroRNA in disease regulation
University of Minnesota
2008-2024
Nanjing Medical University
2014
University of Minnesota Medical Center
2005-2013
Masonic Cancer Center
2007
McGill University
1995
To study the biodistribution of MSCs, we labeled adult murine C57BL/6 MSCs with firefly luciferase and DsRed2 fluorescent protein using nonviral Sleeping Beauty transposons coinfused bone marrow into irradiated allogeneic recipients. Using in vivo whole-body imaging, signals were shown to be increased between weeks 3 12. Unexpectedly, some mice highest died all surviving developed foci sarcoma their lungs. Two also sarcomas extremities. Common cytogenetic abnormalities identified tumor cells...
Recessive dystrophic epidermolysis bullosa (RDEB) is characterized by a functional deficit of type VII collagen protein due to gene defects in the (COL7A1). Gene augmentation therapies are promising, but run risk insertional mutagenesis. To abrogate this risk, we explored possibility using engineered transcription activator-like effector nucleases (TALEN) for precise genome editing. We report ability TALEN induce site-specific double-stranded DNA breaks (DSBs) leading homology-directed...
Background— Induced pluripotent stem cells (iPSCs) can be differentiated into potentially unlimited lineages of cell types for use in autologous therapy. However, the efficiency differentiation procedure and subsequent function iPSC-derived may influenced by epigenetic factors that iPSCs retain from their tissues origin; thus, more effective treatment myocardial injury if were engineered cardiac-lineage cells, rather than dermal fibroblasts. Methods Results— We show human cardiac (hciPSCs)...
Recessive dystrophic epidermolysis bullosa (RDEB) is a severe disorder caused by mutations to the COL7A1 gene that deactivate production of structural protein essential for skin integrity. Haematopoietic cell transplantation can ameliorate some symptoms; however, significant side effects from allogeneic transplant procedure occur and unresponsive areas blistering persist. Therefore, we employed genome editing in patient-derived cells create an autologous platform multilineage engineering...
Revertant mosaicism has been described previously in recessive dystrophic epidermolysis bullosa (RDEB), manifesting as regions of skin with normal mechanical and biological characteristics. Here we report the discovery revertant dermal fibroblasts, unique that all other documented cases occur epidermal keratinocytes. To determine cause found a patient RDEB from isolated keratinocytes fibroblasts blister mosaic regions. Skin biopsies were taken RDEB. Allele identification was confirmed type...
Previous studies have identified Sleeping Beauty transposons as efficient vectors for nonviral gene delivery in mammalian cells. However, demonstrating the usefulness of vehicles into adult stem cells are lacking. Multipotent progenitor (MAPC) nonhematopoietic with capacity to form most, if not all, cell types body and such hold great therapeutic potential. The whole-body biodistribution persistence MAPC unknown, data would help direct clinical applications. We nucleofected murine two...
Spliceosome-mediated RNA trans-splicing (SMaRT) is an emerging technology for the repair of defective pre-messenger (pre-mRNA) molecules. It especially useful in treatment genetic disorders involving large genes. Although viral vectors have been used achieving long-lasting expression molecules, immunogenicity and suboptimal safety profiles associated with viral-based components could limit widespread application SMaRT defects. Here, we tested whether non-viral Sleeping Beauty (SB) transposon...
SUMMARY Neutrophils are present in milk of cows as a means suppressing invading pathogens during mastitis. However, the manner by which neutrophils traverse secretory epithelia is still not clear: do they diapedese between epithelial cells or kill to gain entry into milk? We investigated process bovine neutrophil diapedesis across mammary gland epithelium vitro. The cell line mac-t , grown on collagen-coated filters, formed confluent monolayer with characteristic tight junctions,...