A5089505428- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic and Hepatic Oncology Research
- Peptidase Inhibition and Analysis
- Microscopic Colitis
- Pediatric Hepatobiliary Diseases and Treatments
- Sarcoma Diagnosis and Treatment
- Gastrointestinal motility and disorders
- RNA Research and Splicing
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- Bone Tumor Diagnosis and Treatments
- CRISPR and Genetic Engineering
- Clostridium difficile and Clostridium perfringens research
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Oral and Maxillofacial Pathology
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Galectins and Cancer Biology
- Endoplasmic Reticulum Stress and Disease
- Biotin and Related Studies
- Genomics and Phylogenetic Studies
- Fibroblast Growth Factor Research
- IL-33, ST2, and ILC Pathways
- Genomics and Chromatin Dynamics
Massachusetts General Hospital
2012-2024
Harvard University
2012-2024
Foundation for Applied Molecular Evolution
2007-2010
Harvard University Press
2009
ABSTRACT Osteosarcoma is the most common type primary malignant tumor of bone. Patients with regional osteosarcoma are routinely treated surgery and chemotherapy. In addition, many patients metastatic or recurrent show poor prognosis current chemotherapy agents. Therefore, it important to improve general condition overall survival rate by identifying novel therapeutic strategies. Recent studies have revealed that CDK11 essential in cell growth inhibiting mRNA expression RNAi. Here, we apply...
Significance The burgeoning intestinal microbiota pose a unique challenge to the developing immune system of newborn. Extensive crosstalk between mucosal cells and microbes is necessary establish healthy microbial communities promote development productive immunity. However, it yet unknown whether also influence lymphocytes themselves in thymus. Here, we report that thymic distribution transcription factor PLZF-expressing innate regulated by early life. Migratory plasmacytoid dendritic are...
Abstract Intrahepatic cholangiocarcinoma (ICC) has limited therapeutic options and a dismal prognosis. Adding blockade of the anti–programmed cell death protein (PD)-1 pathway to gemcitabine/cisplatin chemotherapy recently shown efficacy in biliary tract cancers but with low response rates. Here, we studied effects anti–cytotoxic T lymphocyte antigen (CTLA)-4 when combined anti–PD-1 orthotopic murine models ICC. This combination therapy led substantial survival benefits reduction morbidity...
Objective Intrahepatic cholangiocarcinoma (ICC)—a rare liver malignancy with limited therapeutic options—is characterised by aggressive progression, desmoplasia and vascular abnormalities. The aim of this study was to determine the role placental growth factor (PlGF) in ICC progression. Design We evaluated expression PlGF specimens from patients assessed effect genetic or pharmacologic inhibition orthotopically grafted mouse models. impact stimulation blockade cells cancer-associated...
Significance Embryonic fibroblasts have long been considered a relatively homogeneous cell type, and many studies conducted on of varying provenance. The results the present study indicate that embryonic are highly heterogeneous exhibit anatomic developmental variation has both practical consequences for interpretation experimental theoretical implications nature organization metazoan organisms. Studies using to explore altered alleles should take into account possibility fibroblast...
The critical role of the gut microbiome in microscopic colitis (MC) is evident by observation that fecal diversion associated with resolution mucosal inflammation while restoration stream recurrence disease.1 Characterization composition and function MC therefore could provide insights into disease pathogenesis.
Abstract Chordoma is a rare primary malignant bone tumor and there exist only few established human chordoma cell lines. The scarcity of robust lines has limited the ability to study this tumor. In report, we describe establishment novel line characterize its in vitro vivo behaviors. tissue was isolated from patient with recurrent sacrum. After 6 months culture, line, referred here as CH22, established. Microscopic analysis two‐dimensional culture confirmed that CH22 cells exhibited typical...
Chordoma is a rare malignant tumor demonstrating notochordal differentiation. It dependent on brachyury (TBXT), hallmark gene and transcription factor, shares histologic features the same anatomic location as notochord. This study involved molecular comparison of chordoma notochord to identify dysregulated cellular pathways. The lack reference from appropriate control tissue limits our understanding its relationship Therefore, an unbiased chordoma, human notochord, atlas normal cancerous was...
Trypanosoma cruzi, the causative agent of Chagas disease, is surrounded by a mucin coat that plays important functions in parasite survival/invasion and extensively O-glycosylated Golgi cell surface glycosyltransferases. The addition first sugar, α-N-acetylglucosamine (GlcNAc) linked to Threonine (Thr), catalyzed polypeptide α-GlcNAc-transferase (pp-αGlcNAcT) which unstable purification. Here, comparison genomes T. cruzi Dictyostelium discoideum, an amoebazoan also forms this linkage,...
Abstract Osteosarcoma is the most common bone cancer in children and adolescents. Previously, we have found that cyclin-dependent kinase 11 (CDK11) signaling was essential for osteosarcoma cell growth survival. Subsequently, CDK11 siRNA gene targeting, expression profiling, network reconstruction of differentially expressed genes were performed between knock down wild type cells. Reconstructed pointed to AR as key osteosarcoma. increased transcriptional activation lines. protein highly...
Two approaches, one novel, are applied to analyze the divergent evolution of ruminant seminal ribonucleases (RNases), paralogs well-known pancreatic RNases mammals. Here, goal was identify periods divergence RNase under functional constraints, as a pseudogene, and driven by positive selection pressures. The classical approach involves analysis nonsynonymous synonymous replacements ratios (ω) for branches evolutionary tree. novel coupled these analyses with mapping substitutions on folded...
<p>Supplementary Fig. S7: Immunophenotyping in murine 425-ICC and SS49-ICC.</p>
<p>Supplementary Fig. S7: Immunophenotyping in murine 425-ICC and SS49-ICC.</p>
<p>Supplementary Fig. S7: Immunophenotyping in murine 425-ICC and SS49-ICC.</p>
<p>Supplementary Fig. S8: Bulk tissue RNA sequencing analysis of ICC after GC/dual ICB combination therapy in orthotopic murine 425-ICC model.</p>
<p>Supplementary Fig. S9: Expression levels of Cxcr3 and its ligands are increased in ICC tissues after GC/dual ICB treatment murine 425-ICC.</p>
<p>Supplementary Fig. S9: Expression levels of Cxcr3 and its ligands are increased in ICC tissues after GC/dual ICB treatment murine 425-ICC.</p>
<p>Supplementary Fig. S8: Bulk tissue RNA sequencing analysis of ICC after GC/dual ICB combination therapy in orthotopic murine 425-ICC model.</p>