A5006097055- Bone health and osteoporosis research
- Cancer-related gene regulation
- Orthopedic Surgery and Rehabilitation
- Mesenchymal stem cell research
- Foot and Ankle Surgery
- Bone Metabolism and Diseases
- Wnt/β-catenin signaling in development and cancer
- Shoulder Injury and Treatment
- Morphological variations and asymmetry
- Connective tissue disorders research
- Bone fractures and treatments
- Scoliosis diagnosis and treatment
- Hip disorders and treatments
- Orthopaedic implants and arthroplasty
- Bone health and treatments
- Epigenetics and DNA Methylation
- Total Knee Arthroplasty Outcomes
- Pelvic and Acetabular Injuries
- Trauma Management and Diagnosis
- Medical and Biological Sciences
- Lower Extremity Biomechanics and Pathologies
- Pregnancy-related medical research
- Shoulder and Clavicle Injuries
- RNA modifications and cancer
- Urological Disorders and Treatments
Marqués de Valdecilla University Hospital
2014-2025
Universidad de Cantabria
2011-2025
Instituto de Investigación Marqués de Valdecilla
2014-2023
Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable
2015
University of Zulia
1997-2011
To determine genome-wide methylation profiles of bone from patients with hip osteoarthritis (OA) and those osteoporotic (OP) fractures.Trabecular pieces were obtained the central part femoral head 27 fractures 26 OA. DNA was isolated, explored Illumina arrays. RNA extracted, pooled, deep-sequenced to obtain whole transcriptome. Differentially methylated regions identified, connections between genes differentially by pathway text-mining analyses.After quality control, 23,367 CpG sites (13,463...
Insufficient activity of the bone-forming osteoblasts leads to low bone mass and predisposes fragility fractures. The functional capacity human mesenchymal stem cells (hMSCs), precursors osteoblasts, may be compromised in elderly individuals, relation with epigenetic changes associated aging. However, role hMSCs pathogenesis osteoporosis is still unclear. Therefore, we aimed characterize genome-wide methylation gene expression signatures differentiation from patients hip We obtained femoral...
Osteoporosis (OP), characterized by reduced bone mass and mineral density, is a global metabolic disorder that severely impacts the quality of life in affected individuals. Although current pharmacological treatments are effective, their long-term use often associated with adverse effects, highlighting need for safer, more sustainable therapeutic strategies. This study investigates pro-osteogenic anti-resorptive potential secretome from Smurf1-silenced mesenchymal stem cells (MSCs) as...
Genes explaining the susceptibility to osteoporosis have not been fully elucidated. Our objective was explore association of polymorphisms capturing common variations lipoprotein receptor-related protein (LRP) 5 and 6 genes, encoding two Wnt receptors, with femoral neck bone mineral density (BMD) osteoporotic fractures spine hip.Cross-sectional, case-control, replication genetic study.Thirty-nine tagging functional single nucleotide (SNPs) were analyzed in a group 1043 postmenopausal women...
This study explores the genetic factors associated with atypical femoral fractures (AFF), rare prolonged anti-resorptive therapy. AFF are fragility that typically appear in subtrochanteric or diaphyseal regions of femur. While some cases resemble bone disorders, exact cause remains unclear. investigates 457 genes related to skeletal homeostasis 13 patients by exome sequencing, comparing results osteoporotic (n = 27) and Iberian samples from 1000 Genomes Project 107). Only one case carried a...
Long noncoding RNAs (lncRNAs) contribute toward regulating gene expression and cell differentiation may be involved in the pathogenesis of several diseases. The objective this study was to determine patterns lncRNAs bone marrow mesenchymal stem cells (BMSCs) derived from patients with osteoporotic fractures their relevance osteogenic function. BMSCs were isolated femoral head hip (FRX) controls osteoarthritis (OA). We found 74 differentially expressed genes between FRX OA, which 33 lncRNA...
To analyze how balance and other physical capacities evolved after surgery in patients with a bimalleolar fracture these clinical variables (immobilization or unloading time) contribute to restoring patients' functionality, 22 10 healthy people (HC) were assessed for static dynamic (Y-Balance test, YBT), dorsiflexion ankle mobility (ADFROM) hip strength at 6 12 months surgery. Patients' functional status was through the Olerud Molander Ankle Score (OMAS) American Orthopaedic Foot Society...
The purpose of this study was to analyze the regenerative capacity mesenchymal stem cells (MSCs) in treatment fractures. MSCs extracted from patients with osteoporotic hip fractures or osteoarthritis undergoing replacement surgeries were cultured and injected into mice femoral fracture. Two experimental models established, one for systemic administration (n = 29) another local 30). Fracture consolidation assessed by micro-CT histology. degree radiological corticalization better osteoporosis...
Mesenchymal stem cell (MSC) transplantation has emerged as a promising approach for bone regeneration. Importantly, the beneficial effects of MSCs can be improved by modulating expression levels specific genes to stimulate MSC osteogenic differentiation. We have previously shown that Smurf1 silencing using Locked Nucleic Acid-Antisense Oligonucleotides, in combination with scaffold sustainably releases low doses BMP-2, was able increase potential presence BMP-2 significantly smaller than...
The evidence sustaining the regenerative properties of mesenchymal stem cells' (MSCs) secretome has prompted a paradigm change, where MSCs have shifted from being considered direct contributors to tissue regeneration toward seen as cell factories for producing biotech medicines. We previously designed method prime towards osteogenic differentiation by silencing Wnt/β-Catenin inhibitor Sfpr1. This approach produces significant increase in bone formation osteoporotic mice. In this current...
Abstract Fluctuating asymmetry (FA) is an indicator of developmental instability referred to random deviations from mean asymmetry. That average the directional (DA), which, in particular case adolescent idiopathic scoliosis (AIS), corresponds a right thoracic and left lumbar curves. Investigating presence FA DA AIS has never been done, it key element pathophysiology scoliotic condition. Thirty‐six X‐rays patients with were digitized analysed using Geometric Morphometric analyses test for...